##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062066_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198497
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02722963067452	32.0	32.0	32.0	32.0	32.0
2	30.8722650720162	32.0	32.0	32.0	32.0	32.0
3	30.942442455049697	32.0	32.0	32.0	32.0	32.0
4	30.87457744953324	32.0	32.0	32.0	32.0	32.0
5	31.00234764253364	32.0	32.0	32.0	32.0	32.0
6	34.596260900668526	36.0	36.0	36.0	32.0	36.0
7	34.434112354342886	36.0	36.0	36.0	32.0	36.0
8	34.358524310191086	36.0	36.0	36.0	32.0	36.0
9	34.305898829705235	36.0	36.0	36.0	32.0	36.0
10	34.248613329168705	36.0	36.0	36.0	32.0	36.0
11	34.44653571590503	36.0	36.0	36.0	32.0	36.0
12	34.31478057602886	36.0	36.0	36.0	32.0	36.0
13	34.363234708836906	36.0	36.0	36.0	32.0	36.0
14	34.35000528975249	36.0	36.0	36.0	32.0	36.0
15	34.35228744011245	36.0	36.0	36.0	32.0	36.0
16	34.35701295233681	36.0	36.0	36.0	32.0	36.0
17	34.279606240900364	36.0	36.0	36.0	32.0	36.0
18	34.33644337194013	36.0	36.0	36.0	32.0	36.0
19	34.15411819825992	36.0	36.0	36.0	32.0	36.0
20	34.017365501745616	36.0	36.0	36.0	32.0	36.0
21	33.97541020771095	36.0	36.0	36.0	32.0	36.0
22	33.96507251998771	36.0	36.0	36.0	32.0	36.0
23	34.03708368388439	36.0	36.0	36.0	32.0	36.0
24	33.97594925867897	36.0	36.0	36.0	32.0	36.0
25	33.62727396383824	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	4.0
5	12.0
6	37.0
7	8.0
8	39.0
9	25.0
10	18.0
11	3.0
12	9.0
13	11.0
14	158.0
15	168.0
16	190.0
17	245.0
18	307.0
19	419.0
20	547.0
21	711.0
22	894.0
23	1166.0
24	1423.0
25	1664.0
26	2103.0
27	2424.0
28	3194.0
29	4065.0
30	5319.0
31	7343.0
32	10392.0
33	15448.0
34	37543.0
35	102607.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58173464452684	20.29914960502982	12.480352248911815	23.638763501531518
2	11.256089512687849	21.397300714872262	48.44456087497544	18.902048897464446
3	19.729364823472515	28.00660970498146	29.591024504272127	22.6730009672739
4	10.227936879018118	17.674681069751507	38.69233559725105	33.40504645397932
5	9.748636853828401	42.62641227990607	35.08400608754372	12.54094477872182
6	28.26766413937732	40.20003226326827	18.68345700919503	12.848846588159379
7	25.381806443803335	33.82895878306131	21.013981076119194	19.77525369701616
8	23.116224176888313	43.566413815364754	18.621893156875405	14.69546885087153
9	27.826258020257455	14.417799927363706	20.350772769460473	37.405169282918365
10	16.654305851962846	29.83143884928383	32.958977230874794	20.555278067878533
11	33.63741863868005	21.95065341339119	27.465563348302137	16.94636459962662
12	26.597548055093085	24.84536602593209	33.20922254174865	15.347863377226172
13	31.31409949199655	24.366509103199867	26.29359269927911	18.025798705524473
14	19.01413423996933	26.43234027098193	27.5425994491581	27.010926039890638
15	20.006760233682108	39.54080860466759	23.278410638791634	17.174020522858672
16	17.312591112927063	28.971010325711376	34.98332854123475	18.733070020126814
17	16.878203317325156	31.897473667218208	31.073691432261192	20.150631583195448
18	16.77166188345783	27.956300469578288	39.25240715615118	16.01963049081271
19	24.08191876708322	24.476786993050723	28.700817977346766	22.74047626251929
20	24.21916758272235	29.157800256159423	30.69879080649878	15.924241354619442
21	22.00465941868205	26.40589386207314	27.189523367690665	24.399923351554147
22	22.36248190717308	32.932223135619296	28.219262367424335	16.48603258978329
23	18.992396587540085	32.45164673375955	31.279874150414454	17.276082528285905
24	23.274097084245245	27.645712095692428	32.49236013756795	16.587830682494378
25	20.269057391514806	30.26693962344067	32.60253527092304	16.861467714121478
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2063.0
1	2063.0
2	1201.5
3	340.0
4	340.0
5	340.0
6	740.5
7	1141.0
8	1141.0
9	1141.0
10	1102.5
11	1064.0
12	1064.0
13	1064.0
14	909.5
15	755.0
16	755.0
17	755.0
18	1414.0
19	2073.0
20	2073.0
21	2073.0
22	3940.5
23	5808.0
24	5808.0
25	5808.0
26	10074.0
27	14340.0
28	14340.0
29	14340.0
30	19769.5
31	25199.0
32	25199.0
33	25199.0
34	25281.5
35	25364.0
36	25364.0
37	25364.0
38	24406.0
39	23448.0
40	23448.0
41	23448.0
42	23424.0
43	23400.0
44	23400.0
45	23400.0
46	23831.0
47	24262.0
48	24262.0
49	24262.0
50	21999.0
51	19736.0
52	19736.0
53	19736.0
54	15324.5
55	10913.0
56	10913.0
57	10913.0
58	9072.0
59	7231.0
60	7231.0
61	7231.0
62	6107.5
63	4984.0
64	4984.0
65	4984.0
66	4011.5
67	3039.0
68	3039.0
69	3039.0
70	2406.0
71	1773.0
72	1773.0
73	1773.0
74	1342.0
75	911.0
76	911.0
77	911.0
78	632.5
79	354.0
80	354.0
81	354.0
82	249.0
83	144.0
84	144.0
85	144.0
86	95.5
87	47.0
88	47.0
89	47.0
90	29.5
91	12.0
92	12.0
93	12.0
94	12.0
95	12.0
96	12.0
97	12.0
98	48.0
99	84.0
100	84.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.037859514249585E-4
2	0.0
3	5.037859514249585E-4
4	0.01057950497992413
5	0.029723371134072557
6	0.06498838773381965
7	0.11536698287631551
8	0.11335183907061566
9	0.12544270190481469
10	0.146097925913238
11	0.15466228708746227
12	0.1445865680589631
13	0.13602220688473882
14	0.12846541761336444
15	0.14055628044756344
16	0.12796163166193947
17	0.13350327712761403
18	0.1178859126334403
19	0.10226854813926659
20	0.09219282911076741
21	0.09521554481931717
22	0.10781019360494112
23	0.08312468198511816
24	0.09823826052786691
25	0.08816254149936775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	198497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.01869549665738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.11394782324682	24.844707980473256
2	16.068099236537336	11.896401456948972
3	6.377158721302105	7.082222905132067
4	3.2661504334453806	4.836345133679602
5	1.8236006586736706	3.3753658745472226
6	1.111852043385365	2.469558733885147
7	0.7484928076645664	1.9395759129860906
8	0.5538846776717791	1.6403270578396651
9	0.425960452361835	1.4191650251641084
>10	2.148854806004273	14.860677995133429
>50	0.208217090132143	5.40209675712983
>100	0.12520243328207292	9.52508098359169
>500	0.01905254419509805	4.590497589384222
>1k	0.009526272097549025	6.117976594104697
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2021	1.0181514078298413	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1870	0.9420797291646725	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1838	0.9259585787190738	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1829	0.9214245051562492	No Hit
GTCCTACAGTGGACATTTCTAAATT	1696	0.8544209736167298	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1559	0.7854022982715104	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1331	0.6705391013466199	No Hit
GGTATCAACGCAGAGTACTTTTTTT	820	0.413104480168466	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	800	0.40302876113996683	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	775	0.3904341123543429	No Hit
GTCCTACAGTGTGCATTTCTCATTT	715	0.3602069552688454	No Hit
ATTTAGAAATGTCCACTGTAGGACG	715	0.3602069552688454	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	705	0.3551690957545958	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	663	0.3340100857947475	No Hit
CTGAAGGACCTGGAATATGGCGAGA	613	0.30882078822349956	No Hit
GATATACACTGTTCTACAAATCCCG	610	0.30730943036922476	No Hit
TTGTAGAACAGTGTATATCAATGAG	570	0.2871579923122264	No Hit
CTGTAGGACCTGGAATATGGCGAGA	561	0.28262391874940174	No Hit
GTACATGGGGTGGTATCAACGCAAA	539	0.27154062781805266	No Hit
GTGTATATCAATGAGTTACAATGAA	522	0.2629762666438284	No Hit
GAATATGGCAAGAAAACTGAAAATC	504	0.2539081195181791	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	489	0.24635133024680472	No Hit
GATATACACTGTTCTACAATGCCGG	407	0.20504088222995814	No Hit
GTATCAACGCAGAGTACATGGGGTG	406	0.20453709627853317	No Hit
GTGTATATCAATGAGTTACAATGAG	402	0.20252195247283333	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	397	0.20000302271570855	No Hit
GTTCTACAGTGTGGTTTTTATCATT	397	0.20000302271570855	No Hit
GAAATATGGCGAGGAAAACTGAAAA	388	0.19546894915288393	No Hit
GGAATATGGCGAGAAAACTGAAAAT	368	0.18539323012438474	No Hit
GCCATATTCCACGTCCTACAGTGGA	363	0.18287430036725996	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	357	0.1798515846587102	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	335	0.16876829372736113	No Hit
CCATATTTCACGTCCTAAAGTGTGT	315	0.15869257469886194	No Hit
GTTCTACAAATCCCGTTTCCAACGA	311	0.1566774308931621	No Hit
TATCAACGCAGAGTACATGGGGTGG	310	0.15617364494173716	No Hit
GACCTGGAATATGGCGAGAAAACTG	305	0.15365471518461238	No Hit
ATACACACTTTAGGACGTGAAATAT	301	0.15163957137891254	No Hit
GTCCACTGTAGGACGTGGAATATGG	292	0.1471054978160879	No Hit
CTGTAGAACATATTAGATGAGTGAG	286	0.14408278210753814	No Hit
CACTTTAGGACGTGAAATATGGCGA	281	0.14156385235041336	No Hit
CTGTAGGACATGGAATATGGCAAGA	276	0.13904492259328857	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	275	0.1385411366418636	No Hit
ACCTGGAATATGGCGAGAAAACTGA	275	0.1385411366418636	No Hit
CCACTGTAGGACGTGGAATATGGCA	272	0.13702977878758874	No Hit
GTACATGGGTGGTATCAACGCAAAA	270	0.13602220688473882	No Hit
ACAGTGGACATTTCTAAATTTTCCA	261	0.1314881333219142	No Hit
GGTATCAACGCAGAGTACATGGGGT	253	0.12745784571051452	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	248	0.12493891595338973	No Hit
GTATCAACGCAGAGTACATGGGAAA	246	0.12393134405053981	No Hit
GTGTATTTCTCATTTTCCGTGATTT	243	0.12241998619626493	No Hit
GAAATACACACTTTAGGACGTGAAA	243	0.12241998619626493	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	243	0.12241998619626493	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	243	0.12241998619626493	No Hit
ACGCAGAGTACATGGGGTGGTATCA	228	0.11486319692489055	No Hit
GTAGGACGTGGAATATGGCAAGAAA	226	0.11385562502204065	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	223	0.11234426716776576	No Hit
GTCCTACAGTGGACATTTCTAAATA	221	0.11133669526491585	No Hit
GTAGAACATATTAGATGAGTGAGTT	219	0.11032912336206592	No Hit
CTACAGTGGACATTTCTAAATTTTC	216	0.10881776550779106	No Hit
TATTAGATGAGTGAGTTACACTGAA	205	0.1032761200421165	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	199	0.10025340433356675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAAGAGG	25	0.0060079275	19.00328	17
AGATTAT	25	0.0060166963	18.998487	5
CAATATT	30	7.6944014E-4	18.998486	4
TTCTGGA	25	0.006025476	18.993694	2
TATGTCG	35	0.0021587324	16.288527	16
CTCTAGG	55	1.1246464E-5	15.536374	1
TCTAGGA	50	8.677509E-5	15.194956	2
TAGGACC	195	0.0	14.61422	4
GAAATGT	210	0.0	14.475036	6
CCACCTT	145	0.0	14.416283	13
AAGAGGG	40	0.0052428036	14.25606	18
TGTCGAG	40	0.0052428036	14.25606	18
CAAAAGA	40	0.0052515976	14.252461	15
ATATGTC	40	0.0052515976	14.252461	15
GAATATT	40	0.0052515976	14.252461	13
ATGTCGA	40	0.0052515976	14.252461	17
CCAAAAG	40	0.0052515976	14.252461	14
GCCAAAA	40	0.0052515976	14.252461	13
TAGAAAT	210	0.0	14.022691	4
AAATGTC	210	0.0	14.022691	7
>>END_MODULE