FastQCFastQC Report
Thu 2 Feb 2017
SRR4062066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198497
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.5496304730046299No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9710.4891761588336348No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7780.39194547020861775No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC6650.33501765769759745No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA6320.31839272130057383No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6300.3173851493977239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.29723371134072557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.29118827992362606No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.20302573842425833No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG3720.18740837393008458No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA3610.18186672846441004No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA3330.1677607218245112No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC2950.14861685567036279No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC2880.14509035401038806No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT2760.13904492259328857No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA2540.12796163166193947No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA2410.12141241429341502No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA2380.11990105643914013No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.11889348453629023No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2250.11335183907061566No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2160.10881776550779106No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT2090.10529126384781634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTCT207.845593E-443.9944576
ATAATTC250.002349523835.1955643
CGTCCGC250.002349523835.19556410
TGAGACC250.002349523835.19556416
ATACATG1100.034.0300141
TAGAACC551.3654244E-731.9959684
GTCCTAT353.1916844E-431.4563141
GAGTGTG353.2106243E-431.4246147
TATATTG353.2106243E-431.4246145
GCTAGGA353.2106243E-431.42461420
GTCATAT300.005708915629.3592261
AAGGCCG454.0491406E-529.3370337
GATAATT300.00573006629.33702942
TTTCGTA300.00573006629.33702943
CGCGTTT300.00573006629.33702928
TTTCGCG300.005737129629.32963825
AGAACCT602.9276816E-729.3296385
ATGACTT300.005737129629.3296385
AATAGGG300.005737129629.3296387
TACAGCG300.005737129629.3296385
Produced by FastQC (version 0.11.5)