##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198497
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.02638830813564	32.0	32.0	32.0	32.0	32.0
2	31.42695355597314	32.0	32.0	32.0	32.0	32.0
3	31.59672942160335	32.0	32.0	32.0	32.0	32.0
4	31.72800092696615	32.0	32.0	32.0	32.0	32.0
5	31.7110132646841	32.0	32.0	32.0	32.0	32.0
6	35.37795029647803	36.0	36.0	36.0	36.0	36.0
7	35.36523977692358	36.0	36.0	36.0	36.0	36.0
8	35.34828737965813	36.0	36.0	36.0	36.0	36.0
9	35.35545625374691	36.0	36.0	36.0	36.0	36.0
10	35.28415039018222	36.0	36.0	36.0	36.0	36.0
11	35.382030962684574	36.0	36.0	36.0	36.0	36.0
12	35.32891680982584	36.0	36.0	36.0	36.0	36.0
13	35.346725643208714	36.0	36.0	36.0	36.0	36.0
14	35.30142521045658	36.0	36.0	36.0	36.0	36.0
15	35.30203982931732	36.0	36.0	36.0	36.0	36.0
16	35.31102737069074	36.0	36.0	36.0	36.0	36.0
17	35.300886159488556	36.0	36.0	36.0	36.0	36.0
18	35.293843231887635	36.0	36.0	36.0	36.0	36.0
19	35.215731220119196	36.0	36.0	36.0	36.0	36.0
20	35.150979611782546	36.0	36.0	36.0	36.0	36.0
21	35.0931953631541	36.0	36.0	36.0	36.0	36.0
22	35.06620251187675	36.0	36.0	36.0	36.0	36.0
23	35.096374252507594	36.0	36.0	36.0	36.0	36.0
24	35.081084348881845	36.0	36.0	36.0	36.0	36.0
25	35.01642845987597	36.0	36.0	36.0	36.0	36.0
26	35.03760258341436	36.0	36.0	36.0	36.0	36.0
27	34.955676911993635	36.0	36.0	36.0	36.0	36.0
28	34.88770107356786	36.0	36.0	36.0	36.0	36.0
29	34.808788042136655	36.0	36.0	36.0	32.0	36.0
30	34.719245127130385	36.0	36.0	36.0	32.0	36.0
31	34.71237852461246	36.0	36.0	36.0	32.0	36.0
32	34.66175307435377	36.0	36.0	36.0	32.0	36.0
33	34.66132989415457	36.0	36.0	36.0	32.0	36.0
34	34.59361098656403	36.0	36.0	36.0	32.0	36.0
35	34.55588245666181	36.0	36.0	36.0	32.0	36.0
36	34.48013320100556	36.0	36.0	36.0	32.0	36.0
37	34.32424671405613	36.0	36.0	36.0	32.0	36.0
38	34.28230653360001	36.0	36.0	36.0	32.0	36.0
39	33.94087064288125	36.0	36.0	36.0	32.0	36.0
40	34.100656433094706	36.0	36.0	36.0	32.0	36.0
41	34.05311415285873	36.0	36.0	36.0	32.0	36.0
42	34.02911882799236	36.0	36.0	36.0	32.0	36.0
43	34.00417638553731	36.0	36.0	36.0	32.0	36.0
44	33.78435946135206	36.0	36.0	36.0	32.0	36.0
45	33.71045406227802	36.0	36.0	36.0	27.0	36.0
46	33.3940966362212	36.0	36.0	36.0	21.0	36.0
47	33.36219187191746	36.0	36.0	36.0	21.0	36.0
48	33.23159543972957	36.0	36.0	36.0	14.0	36.0
49	33.09974961838214	36.0	36.0	36.0	14.0	36.0
50	32.837997551600274	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	3.0
21	12.0
22	33.0
23	55.0
24	150.0
25	334.0
26	759.0
27	1515.0
28	2609.0
29	4047.0
30	5707.0
31	7696.0
32	11037.0
33	17243.0
34	33203.0
35	114088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72562893081761	20.79100145137881	13.449947589098532	24.033422028705047
2	10.995123130869372	20.94232396920721	48.56364112691951	19.49891177300391
3	19.66679597172753	27.70721975646987	30.31330448319118	22.312679788611415
4	10.059094092102146	17.44761885570059	39.07817246608261	33.415114586114655
5	9.60619052177111	42.03841871665567	35.13403225237661	13.221358509196612
6	28.606118007415766	40.082923585361925	18.35855634370466	12.952402063517654
7	25.28753583177579	33.63174254522738	21.439618734791964	19.64110288820486
8	22.94644251550401	42.77948785120178	19.089961057345956	15.184108575948251
9	27.51578109492839	13.557887524748486	20.755477412756868	38.17085396756626
10	16.7272049451629	29.286084928235688	33.10881272764828	20.87789739895313
11	33.47103482672282	21.506118480380056	28.17070283178083	16.85214386111629
12	26.554557499609565	24.196839246940762	33.859957581222886	15.388645672226783
13	31.599973803130528	23.844692866894714	26.57722786742369	17.97810546255107
14	19.099029204471606	25.58829604477649	28.20395270457488	27.108722046177018
15	20.039597575782	38.580431946074754	24.21346418333778	17.166506294805465
16	17.176251290964508	28.040001007582056	35.65530617899695	19.128441522456484
17	16.61737960775226	31.17679360393356	31.940029320342372	20.265797467971808
18	16.81494841205868	26.706835402224733	40.394768660325646	16.08344752539094
19	23.943434913374006	23.163574260568172	30.364186864285102	22.528803961772724
20	24.189282457669385	27.945510511493875	32.148596704232304	15.716610326604433
21	21.69856471382439	25.09609717023431	28.915298467986922	24.29003964795438
22	22.397819614402234	31.292160586809874	30.22363058383754	16.08638921495035
23	18.89852239580447	30.81306014700474	32.85843110979007	17.429986347400718
24	23.114823472513297	26.3415887473803	33.74677575366758	16.796812026438822
25	20.587716690932357	28.63972755255747	33.90026045733689	16.872295299173288
26	15.26924840173907	31.508284759971183	35.85746887862285	17.364997959666898
27	17.10672812917202	30.301518929947857	34.25124058540517	18.34051235547495
28	16.571952522016	31.570038087177316	31.09898635713278	20.759023033673902
29	19.32290485906446	26.823345676213506	30.14483992040102	23.708909544321017
30	16.626195250234264	28.791802271101393	33.031728918758255	21.550273559906095
31	21.764779969268748	26.125595103151213	32.16705710471297	19.942567822867076
32	17.854857804982494	31.055694098088114	32.61643870122673	18.473009395702665
33	16.903530028161633	30.843791090041663	35.603056973153244	16.649621908643457
34	18.32832903433822	30.432855070127356	34.81581492826052	16.4230009672739
35	16.960457840672653	32.059930376781516	33.55214436490224	17.42746741764359
36	16.001833790101564	30.719006932129616	35.30902385942286	17.97013541834596
37	16.741983425275194	30.36298143530064	30.62545656061866	22.26957857880551
38	18.8567081618362	33.46196667959717	29.955112671728035	17.72621248683859
39	21.66732998483604	27.679007743190077	31.68460984296992	18.969052429003966
40	23.39128551061225	28.119820450686912	32.30275520536834	16.186138833332496
41	22.070973722392388	26.278111397710784	35.0898758665162	16.561039013380622
42	19.041453387610336	32.812059167304824	32.69114505662811	15.455342388456733
43	16.100721454193703	33.37682882592399	33.92598041191407	16.59646930796824
44	15.779529939290123	32.762677280399025	31.960601556792707	19.49719122351815
45	16.568762783761727	33.05036928068355	31.196912753030322	19.18395518252441
46	18.51963807105576	28.95179654595651	30.71811458396308	21.810450799024647
47	22.262525504420765	29.40426711000277	29.838031184664597	18.49517620091186
48	17.26349411065322	31.145525809344367	28.95704656060133	22.63393351940109
49	21.044676863551175	29.55988150655946	29.51907381657699	19.876367813312378
50	17.835299807550857	34.98745554021784	27.585720475178093	19.59152417705321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1217.0
1	652.5
2	88.0
3	223.0
4	358.0
5	336.5
6	315.0
7	207.5
8	100.0
9	192.0
10	284.0
11	465.0
12	646.0
13	1154.0
14	1662.0
15	2113.5
16	2565.0
17	2593.5
18	2622.0
19	2399.0
20	2176.0
21	2246.0
22	2316.0
23	2152.5
24	1989.0
25	2285.5
26	2582.0
27	4080.5
28	5579.0
29	7029.0
30	8479.0
31	10152.0
32	11825.0
33	15086.0
34	18347.0
35	21503.0
36	24659.0
37	23751.0
38	22843.0
39	19737.5
40	16632.0
41	13859.0
42	11086.0
43	9234.5
44	7383.0
45	6740.5
46	6098.0
47	5983.5
48	5869.0
49	5727.5
50	5586.0
51	5701.0
52	5816.0
53	5671.5
54	5527.0
55	5393.5
56	5260.0
57	4862.0
58	4464.0
59	4168.0
60	3872.0
61	3423.0
62	2974.0
63	2524.0
64	2074.0
65	1880.0
66	1686.0
67	1455.0
68	1224.0
69	1067.0
70	910.0
71	719.5
72	529.0
73	457.0
74	385.0
75	297.0
76	209.0
77	170.0
78	131.0
79	93.0
80	55.0
81	46.0
82	37.0
83	30.0
84	23.0
85	16.5
86	10.0
87	5.5
88	1.0
89	1.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032746086842622306
2	0.004534073562824627
3	0.0
4	0.0
5	0.0
6	5.037859514249585E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.001007571902849917
17	0.0
18	5.037859514249585E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	5.037859514249585E-4
25	0.0
26	0.0
27	0.001007571902849917
28	0.002518929757124793
29	0.001007571902849917
30	0.0015113578542748757
31	0.001007571902849917
32	0.001007571902849917
33	0.0
34	5.037859514249585E-4
35	0.0
36	5.037859514249585E-4
37	0.001007571902849917
38	0.0
39	0.0
40	0.0
41	5.037859514249585E-4
42	0.004534073562824627
43	0.004534073562824627
44	0.006045431417099503
45	0.0015113578542748757
46	0.002518929757124793
47	0.001007571902849917
48	0.0015113578542748757
49	0.002518929757124793
50	0.0015113578542748757
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	198497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.46926150017381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.38143489994398	35.31186869322962
2	14.475278782015376	14.321627027108722
3	5.681552013849992	8.431865468999531
4	2.8097153622893223	5.559781759925843
5	1.6273741025510464	4.0252497518854184
6	0.9725546107235602	2.8866935016650124
7	0.6303783288354804	2.1829045275243453
8	0.4134630072814298	1.6362967702282654
9	0.337084372931412	1.5007783492949516
>10	1.5112785783390192	13.122616462717321
>50	0.10081979734202352	3.4665511317551396
>100	0.05091908956667855	4.5637969339587
>500	0.007128672539334997	2.4403391487024995
>1k	0.001018381791333571	0.5496304730046299
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1091	0.5496304730046299	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	971	0.4891761588336348	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	778	0.39194547020861775	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	665	0.33501765769759745	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	632	0.31839272130057383	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	630	0.3173851493977239	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	590	0.29723371134072557	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	578	0.29118827992362606	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.20302573842425833	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	372	0.18740837393008458	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	361	0.18186672846441004	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	333	0.1677607218245112	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	295	0.14861685567036279	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	288	0.14509035401038806	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	276	0.13904492259328857	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	254	0.12796163166193947	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	241	0.12141241429341502	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	238	0.11990105643914013	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	236	0.11889348453629023	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	225	0.11335183907061566	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	216	0.10881776550779106	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	209	0.10529126384781634	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0015113578542748757	0.0
22	0.0	0.0	0.0	0.0015113578542748757	0.0
23	0.0	0.0	0.0	0.004030287611399668	0.0
24	0.0	0.0	0.0	0.005037859514249586	0.0
25	0.0	0.0	0.0	0.007556789271374378	0.0
26	0.0	0.0	0.0	0.009571933077074213	0.0
27	0.0	0.0	0.0	0.013098434737048923	0.0
28	0.0	0.0	0.0	0.016624936397023633	0.0
29	0.0	0.0	0.0	0.023174153765548093	0.0
30	0.0	0.0	0.0	0.03728016040544693	0.0
31	0.0	0.0	0.0	0.06700353153951949	0.0
32	0.0	0.0	0.0	0.09571933077074213	0.0
33	0.0	0.0	0.0	0.11536698287631551	0.0
34	0.0	0.0	0.0	0.13299949117618906	0.0
35	0.0	0.0	0.0	0.15869257469886194	0.0
36	0.0	0.0	0.0	0.19244623344433417	0.0
37	0.0	0.0	0.0	0.23375668146118078	0.0
38	0.0	0.0	0.0	0.279601203040852	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACTCT	20	7.845593E-4	43.994457	6
ATAATTC	25	0.0023495238	35.195564	3
CGTCCGC	25	0.0023495238	35.195564	10
TGAGACC	25	0.0023495238	35.195564	16
ATACATG	110	0.0	34.030014	1
TAGAACC	55	1.3654244E-7	31.995968	4
GTCCTAT	35	3.1916844E-4	31.456314	1
GAGTGTG	35	3.2106243E-4	31.424614	7
TATATTG	35	3.2106243E-4	31.424614	5
GCTAGGA	35	3.2106243E-4	31.424614	20
GTCATAT	30	0.0057089156	29.359226	1
AAGGCCG	45	4.0491406E-5	29.33703	37
GATAATT	30	0.005730066	29.337029	42
TTTCGTA	30	0.005730066	29.337029	43
CGCGTTT	30	0.005730066	29.337029	28
TTTCGCG	30	0.0057371296	29.329638	25
AGAACCT	60	2.9276816E-7	29.329638	5
ATGACTT	30	0.0057371296	29.329638	5
AATAGGG	30	0.0057371296	29.329638	7
TACAGCG	30	0.0057371296	29.329638	5
>>END_MODULE