FastQCFastQC Report
Thu 2 Feb 2017
SRR4062065_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062065_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345773
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19710.1464585780811474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACC453.533203E-516.8917358
GGTATCA3750.015.9531891
TACCGTC456.7547604E-414.7797177
AGTTGCG400.00528183514.24868917
TAAGACT853.9396873E-612.2948174
GTATCAA10950.012.2276561
GCGTGCG550.003063352812.0933959
GGCGTGC658.010854E-411.6942788
TGGACCG752.0708909E-411.4014975
AGGACTG3250.011.4014965
TAGGACT1001.9274303E-611.4006484
GTCTTTA1001.938677E-611.3951361
TATTAAG1104.978756E-711.2249862
TAGAGGT1553.1286618E-1011.0328854
TCTAGAT951.3605302E-510.9993973
GCTATAC700.00149185710.8565473
CCCCTAG1252.2515815E-710.6354591
GTGTAAG1252.2515815E-710.6354591
GTATAAG1252.2515815E-710.6354591
ACCGCTT909.495723E-510.5573348
Produced by FastQC (version 0.11.5)