FastQCFastQC Report
Thu 2 Feb 2017
SRR4062065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345773
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1050.029.3344231
GGTATCA5550.026.1631341
GTATCAA12200.025.9681781
GTATTAG755.4716606E-520.5340961
CGTAACA654.932564E-420.3076922
TATACCG654.932564E-420.3076925
CTTACGA550.004483870720.02
CGCGGAT1404.0745363E-918.85994542
ACGTAAC600.007410937518.3340171
GTATTAC1456.3064363E-918.2075731
TATCAAC17900.017.6983242
GCACCGT2003.6379788E-1217.66
TGTTACG750.001290973517.62
ATCAACG17900.017.5747663
TCAACGC17950.017.5264634
CTATATA1408.3740815E-817.2857152
CAACGCA18500.017.0054055
GTATAAG2251.8189894E-1216.622841
TCGCGGA1602.1276719E-816.50122641
CCTCGCG1753.623427E-916.34407239
Produced by FastQC (version 0.11.5)