##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1345773
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278283187432056	32.0	32.0	32.0	32.0	32.0
2	31.492224171535614	32.0	32.0	32.0	32.0	32.0
3	31.596157747257525	32.0	32.0	32.0	32.0	32.0
4	31.66854365483629	32.0	32.0	32.0	32.0	32.0
5	31.64555240742681	32.0	32.0	32.0	32.0	32.0
6	35.302580747273126	36.0	36.0	36.0	36.0	36.0
7	35.31062965299497	36.0	36.0	36.0	36.0	36.0
8	35.26193942068982	36.0	36.0	36.0	36.0	36.0
9	35.34600114580988	36.0	36.0	36.0	36.0	36.0
10	35.23324513123684	36.0	36.0	36.0	36.0	36.0
11	35.34093194023063	36.0	36.0	36.0	36.0	36.0
12	35.28042990905598	36.0	36.0	36.0	36.0	36.0
13	35.2983296588652	36.0	36.0	36.0	36.0	36.0
14	35.27033831114163	36.0	36.0	36.0	36.0	36.0
15	35.24628819273384	36.0	36.0	36.0	36.0	36.0
16	35.26445098839106	36.0	36.0	36.0	36.0	36.0
17	35.242193148473035	36.0	36.0	36.0	36.0	36.0
18	35.236145323171144	36.0	36.0	36.0	36.0	36.0
19	35.23521351669264	36.0	36.0	36.0	36.0	36.0
20	35.23137185840405	36.0	36.0	36.0	36.0	36.0
21	35.22962044861949	36.0	36.0	36.0	36.0	36.0
22	35.205510141754964	36.0	36.0	36.0	36.0	36.0
23	35.17775360331943	36.0	36.0	36.0	36.0	36.0
24	35.17514395072572	36.0	36.0	36.0	36.0	36.0
25	35.16263961306996	36.0	36.0	36.0	36.0	36.0
26	35.11317138923132	36.0	36.0	36.0	36.0	36.0
27	35.10640576085269	36.0	36.0	36.0	36.0	36.0
28	35.09088977115754	36.0	36.0	36.0	36.0	36.0
29	35.0801152943327	36.0	36.0	36.0	36.0	36.0
30	35.06119382689354	36.0	36.0	36.0	36.0	36.0
31	35.0510769646887	36.0	36.0	36.0	36.0	36.0
32	35.03744465076948	36.0	36.0	36.0	36.0	36.0
33	35.00863815814405	36.0	36.0	36.0	36.0	36.0
34	35.005958657217825	36.0	36.0	36.0	36.0	36.0
35	34.990505085181525	36.0	36.0	36.0	36.0	36.0
36	34.97207998674368	36.0	36.0	36.0	36.0	36.0
37	34.966695720600725	36.0	36.0	36.0	36.0	36.0
38	34.95024643829234	36.0	36.0	36.0	36.0	36.0
39	34.9383380406651	36.0	36.0	36.0	36.0	36.0
40	34.92138941708594	36.0	36.0	36.0	36.0	36.0
41	34.897299173040324	36.0	36.0	36.0	36.0	36.0
42	34.87079173084911	36.0	36.0	36.0	36.0	36.0
43	34.85951345434928	36.0	36.0	36.0	36.0	36.0
44	34.84220370002965	36.0	36.0	36.0	32.0	36.0
45	34.82037609611725	36.0	36.0	36.0	32.0	36.0
46	34.80719408102258	36.0	36.0	36.0	32.0	36.0
47	34.77899616057091	36.0	36.0	36.0	32.0	36.0
48	34.7605680898636	36.0	36.0	36.0	32.0	36.0
49	34.74583677930825	36.0	36.0	36.0	32.0	36.0
50	34.33682203462248	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	25.0
22	81.0
23	222.0
24	590.0
25	1444.0
26	3004.0
27	5557.0
28	9421.0
29	15510.0
30	23135.0
31	34240.0
32	51664.0
33	87673.0
34	202514.0
35	910684.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05548663416418	17.34879022517102	12.042520327680952	26.55320281298385
2	16.08508842198816	19.359949350006612	37.18665146770543	27.368310760299796
3	19.255593452075765	22.11195077910787	28.06928375576807	30.56317201304829
4	13.476354092669487	15.456704404609397	33.89519175610727	37.17174974661384
5	15.248559749675467	35.957772967655025	33.042719686009455	15.750947596660062
6	36.20390378162126	33.62397196553354	15.91859542329607	14.253528829549136
7	31.48874290092014	29.834526327991423	19.33015449113632	19.346576279952117
8	28.383092839579927	33.914560627981096	18.340091531038297	19.362255001400683
9	26.914568801722133	14.8703384597551	18.174759041829493	40.040333696693274
10	16.42547866684401	27.3613079213446	31.35315198968025	24.86006142213114
11	37.29744912403503	21.27892296843524	21.429988564193216	19.993639343336504
12	24.594786787964985	23.320351946427813	28.62875091118636	23.456110354420844
13	30.20353358255813	19.61125687615965	24.27222124385019	25.912988297432033
14	23.203244529352276	19.14840021311172	25.054373954597096	32.59398130293891
15	25.151492859494134	26.860176270440856	21.70410611596458	26.284224754100432
16	25.80664097621726	26.114941230496235	23.017671720046426	25.060746073240075
17	24.572494766948065	25.697944601355506	24.976574801248056	24.752985830448374
18	24.725621763005563	25.609130832163	25.221025881094093	24.44422152373734
19	25.69305944840582	24.904218545191906	25.06761918066359	24.335102825738684
20	25.592857620544372	25.053240895546786	25.14768496845672	24.20621651545212
21	25.77910671347004	24.55557107741876	24.840017476957463	24.82530473215374
22	25.783545962060465	24.697627311589695	24.699039139587434	24.819787586762406
23	25.360146176212485	24.473518193632955	24.985565916391547	25.18076971376302
24	24.93726579166967	25.054448908681138	25.081125344322935	24.927159955326257
25	25.294069566121998	24.34762255065873	25.138322298758332	25.21998558446094
26	25.162305695727998	24.88499089364175	25.0107188264204	24.94198458420985
27	25.15091754299404	25.16823107697612	24.72179454408189	24.959056835947948
28	25.426512233780496	24.703862035859796	24.739455809104427	25.13016992125528
29	25.12041505110116	25.314431526525492	24.901877152126453	24.663276270246897
30	25.31764838762652	24.832193819204026	24.91601331303237	24.934144480137082
31	25.500682140405008	24.585811479575913	24.854357822025257	25.059148557993822
32	25.256807326142333	24.973769390587893	24.876054797452138	24.89336848581764
33	25.188907143658597	24.684362151843523	25.04014439349456	25.086586311003316
34	25.22886695993342	24.83563191059327	25.024075615265723	24.911425514207586
35	25.157939320983523	24.83098895056362	24.899797142156537	25.11127458629632
36	25.2130750425407	24.86353537380087	25.166484614755863	24.75690496890256
37	25.381718528470245	24.698236530247684	24.721792063345806	25.198252877936266
38	25.278260152343673	25.080158392239998	24.882056632136326	24.759524823280003
39	25.172782509899562	24.88930104542614	24.928460870269518	25.009455574404782
40	25.56190796063523	24.70009778773819	24.860080310780727	24.87791394084585
41	25.288703252216777	24.64415991273326	24.999647039328735	25.067489795721226
42	25.20771547531379	24.574667220525058	24.92570841733478	25.29190888682637
43	25.40394973070018	24.670954855125018	24.728470876383653	25.196624537791145
44	24.997956456927316	24.887828062973824	25.048339082501176	25.065876397597687
45	25.313655494655745	24.95184819698249	24.732415674898046	25.00208063346372
46	25.332362371771772	24.7922897725795	24.742428914838992	25.13291894080974
47	25.120285460153653	24.99448265920222	24.817258985091662	25.067972895552465
48	24.96299345771891	25.072155326415533	24.849372969470345	25.115478246395206
49	25.24031673656708	24.735086838014332	24.83072309032164	25.193873335096946
50	24.789262179843476	25.17009128009107	25.018279796811882	25.022366743253567
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	40.5
2	52.0
3	128.5
4	205.0
5	251.5
6	298.0
7	222.0
8	146.0
9	206.0
10	266.0
11	447.0
12	628.0
13	1091.5
14	1555.0
15	1977.0
16	2399.0
17	2309.5
18	2220.0
19	2137.0
20	2054.0
21	2549.5
22	3045.0
23	3665.0
24	4285.0
25	5743.0
26	7201.0
27	8710.0
28	10219.0
29	12370.0
30	14521.0
31	16720.0
32	18919.0
33	22512.5
34	26106.0
35	30148.0
36	34190.0
37	38256.5
38	42323.0
39	47944.5
40	53566.0
41	58178.0
42	62790.0
43	68103.0
44	73416.0
45	80724.5
46	88033.0
47	92804.5
48	97576.0
49	101128.0
50	104680.0
51	108657.0
52	112634.0
53	113489.0
54	114344.0
55	109739.5
56	105135.0
57	101412.5
58	97690.0
59	88015.0
60	78340.0
61	69399.0
62	60458.0
63	52864.0
64	45270.0
65	37850.5
66	30431.0
67	25845.5
68	21260.0
69	17020.5
70	12781.0
71	9892.5
72	7004.0
73	5467.5
74	3931.0
75	3323.0
76	2715.0
77	2139.0
78	1563.0
79	1220.0
80	877.0
81	607.5
82	338.0
83	237.0
84	136.0
85	109.0
86	82.0
87	52.5
88	23.0
89	16.0
90	9.0
91	7.0
92	5.0
93	6.0
94	7.0
95	7.5
96	8.0
97	9.0
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032694964158145545
2	0.0049785513604448895
3	2.2292021016917415E-4
4	7.430673672305805E-5
5	0.0
6	7.430673672305805E-5
7	0.0
8	0.0
9	0.0
10	2.972269468922322E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.201471570614063E-4
17	0.0
18	2.2292021016917415E-4
19	7.430673672305805E-5
20	2.2292021016917415E-4
21	7.430673672305805E-5
22	0.0
23	0.0
24	0.0011889077875689288
25	2.2292021016917415E-4
26	7.430673672305805E-4
27	3.7153368361529025E-4
28	0.0023778155751378576
29	8.173741039536386E-4
30	0.0017833616813533932
31	6.687606305075225E-4
32	0.0012632145242919867
33	4.458404203383483E-4
34	9.659875773997547E-4
35	2.2292021016917415E-4
36	2.2292021016917415E-4
37	0.0012632145242919867
38	0.0
39	4.458404203383483E-4
40	7.430673672305805E-5
41	0.0010402943141228126
42	0.004607017676829599
43	0.004532710940106541
44	0.005275778307337121
45	0.0021548953649686833
46	0.002080588628245625
47	0.0011889077875689288
48	0.004829937886998773
49	0.0036410300994298445
50	0.0018576684180764513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1345773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.214288463787895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.485111974307806	23.921514522355377
2	17.092693102732667	13.747409821125785
3	7.94040708960184	9.579534636634648
4	4.57651046492677	7.361644479764368
5	2.8156177814724237	5.661403283395129
6	1.8580494410409438	4.4832081721200225
7	1.291648893279229	3.6359918831784315
8	0.9414291738727921	3.0287123493076797
9	0.7004062013266636	2.5349703319778714
>10	3.160547382099832	21.28508180214995
>50	0.10951481921799876	2.9617864828107185
>100	0.02750612370708846	1.6451544559623925
>500	5.575524139535019E-4	0.15358777921759073
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.430673672305805E-5
2	0.0	0.0	0.0	0.0	7.430673672305805E-5
3	0.0	0.0	0.0	0.0	7.430673672305805E-5
4	0.0	0.0	0.0	7.430673672305805E-5	7.430673672305805E-5
5	0.0	0.0	0.0	7.430673672305805E-5	7.430673672305805E-5
6	2.972269468922322E-4	0.0	0.0	7.430673672305805E-5	7.430673672305805E-5
7	5.201471570614063E-4	0.0	0.0	7.430673672305805E-5	7.430673672305805E-5
8	5.201471570614063E-4	0.0	0.0	7.430673672305805E-5	7.430673672305805E-5
9	5.201471570614063E-4	0.0	0.0	7.430673672305805E-5	7.430673672305805E-5
10	5.944538937844644E-4	0.0	0.0	7.430673672305805E-5	1.486134734461161E-4
11	5.944538937844644E-4	0.0	0.0	1.486134734461161E-4	1.486134734461161E-4
12	5.944538937844644E-4	0.0	0.0	1.486134734461161E-4	2.2292021016917415E-4
13	5.944538937844644E-4	0.0	0.0	2.972269468922322E-4	2.972269468922322E-4
14	0.0010402943141228126	0.0	0.0	3.7153368361529025E-4	2.972269468922322E-4
15	0.0010402943141228126	0.0	0.0	6.687606305075224E-4	2.972269468922322E-4
16	0.0011889077875689288	0.0	0.0	7.430673672305805E-4	2.972269468922322E-4
17	0.001263214524291987	0.0	0.0	8.916808406766966E-4	4.458404203383483E-4
18	0.0013375212610150448	0.0	0.0	0.001263214524291987	4.458404203383483E-4
19	0.001709054944630335	0.0	0.0	0.001634748207907277	4.458404203383483E-4
20	0.001709054944630335	0.0	0.0	0.0022292021016917414	4.458404203383483E-4
21	0.001709054944630335	0.0	0.0	0.002823655995476206	4.458404203383483E-4
22	0.0018576684180764513	0.0	0.0	0.0034924166259837283	4.458404203383483E-4
23	0.0018576684180764513	0.0	0.0	0.0042354839932143085	4.458404203383483E-4
24	0.002526429048583974	0.0	0.0	0.00572161872767547	4.458404203383483E-4
25	0.002526429048583974	0.0	0.0	0.007727900619198037	4.458404203383483E-4
26	0.002526429048583974	0.0	0.0	0.009808489247443663	4.458404203383483E-4
27	0.002526429048583974	0.0	0.0	0.011368930718627882	4.458404203383483E-4
28	0.002823655995476206	0.0	0.0	0.013226599136704332	4.458404203383483E-4
29	0.0031208829423684382	0.0	0.0	0.0175363898666417	4.458404203383483E-4
30	0.003269496415814554	0.0	0.0	0.0235552355412094	4.458404203383483E-4
31	0.003269496415814554	0.0	0.0	0.03321511131520695	5.201471570614063E-4
32	0.003269496415814554	0.0	0.0	0.04458404203383483	5.201471570614063E-4
33	0.003343803152537612	0.0	0.0	0.05810786811743139	5.201471570614063E-4
34	0.00341810988926067	0.0	0.0	0.07029417294001292	5.201471570614063E-4
35	0.003938257046322077	0.0	0.0	0.08627012133547039	5.201471570614063E-4
36	0.004309790729937367	0.0	0.0	0.10692739414448053	5.201471570614063E-4
37	0.004384097466660425	0.0	0.0	0.1370959292540421	5.201471570614063E-4
38	0.004532710940106541	0.0	0.0	0.17469513803590947	5.201471570614063E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGG	105	0.0	29.334423	1
GGTATCA	555	0.0	26.163134	1
GTATCAA	1220	0.0	25.968178	1
GTATTAG	75	5.4716606E-5	20.534096	1
CGTAACA	65	4.932564E-4	20.307692	2
TATACCG	65	4.932564E-4	20.307692	5
CTTACGA	55	0.0044838707	20.0	2
CGCGGAT	140	4.0745363E-9	18.859945	42
ACGTAAC	60	0.0074109375	18.334017	1
GTATTAC	145	6.3064363E-9	18.207573	1
TATCAAC	1790	0.0	17.698324	2
GCACCGT	200	3.6379788E-12	17.6	6
TGTTACG	75	0.0012909735	17.6	2
ATCAACG	1790	0.0	17.574766	3
TCAACGC	1795	0.0	17.526463	4
CTATATA	140	8.3740815E-8	17.285715	2
CAACGCA	1850	0.0	17.005405	5
GTATAAG	225	1.8189894E-12	16.62284	1
TCGCGGA	160	2.1276719E-8	16.501226	41
CCTCGCG	175	3.623427E-9	16.344072	39
>>END_MODULE