FastQCFastQC Report
Thu 2 Feb 2017
SRR4062064_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062064_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948404
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA46780.4932497121479876No Hit
GTCCTACAGTGGACATTTCTAAATT46560.4909300256009042No Hit
GTCCTAAAGTGTGTATTTCTCATTT40530.4273495261513026No Hit
CTTTAGGACGTGAAATATGGCGAGG33760.35596644467969346No Hit
GTCCTACAGTGTGCATTTCTCATTT31720.3344566239703755No Hit
CTGTAGGACCTGGAATATGGCGAGA24430.25759064702384216No Hit
GCGCAAGACGGACCAGAGCGAAAGC17610.18568036406425953No Hit
GTATCAACGCAGAGTACTTTTTTTT16630.17534721489997931No Hit
GATATACACTGTTCTACAAATCCCG15950.16817727466354002No Hit
CTGAAGGACCTGGAATATGGCGAGA15530.1637487821645628No Hit
GTCCTTCAGTGTGCATTTCTCATTT15080.15900396877280146No Hit
ATTTAGAAATGTCCACTGTAGGACG14670.1546809165714189No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14210.14983066288206293No Hit
GAATAGGACCGCGGTTCTATTTTGT14080.14845993901333188No Hit
GGGTAGGCACACGCTGAGCCAGTCA13890.14645657335903264No Hit
TTGTAGAACAGTGTATATCAATGAG12930.13633430478994182No Hit
GATTAAGAGGGACGGCCGGGGGCAT12710.1340146182428585No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12460.13137861080299112No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC12420.13095684961261234No Hit
TCGTAGTTCCGACCATAAACGATGC12300.129691566041476No Hit
GAATATGGCAAGAAAACTGAAAATC12050.1270555586016086No Hit
ATCAGATACCGTCGTAGTTCCGACC11980.12631747651844571No Hit
GTGTATATCAATGAGTTACAATGAA11700.12336514818579424No Hit
GAACTACGACGGTATCTGATCGTCT11580.12209986461465788No Hit
TTTCTAAATTTTCCACCTTTTTCAG11550.1217835437218738No Hit
GTATCTGATCGTCTTCGAACCTCCG11510.12136178253149502No Hit
TATCAACGCAGAGTACTTTTTTTTT11230.11840945419884354No Hit
GAATAACGCCGCCGCATCGCCAGTC10940.11535168556859735No Hit
CTGTAGGACATGGAATATGGCAAGA10890.11482448408062387No Hit
ACCATACTCCCCCCGGAACCCAAAG10750.11334831991429813No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT10490.11060687217683604No Hit
GTGTATATCAATGAGTTACAATGAG10340.1090252677129156No Hit
GTGCATGGCCGTTCTTAGTTGGTGG9950.10491309610672246No Hit
GCCATGCACCACCACCCACGGAATC9640.10164444688128688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTC402.757641E-416.62661710
CGACCAT2000.015.67652410
AGCCCCG1700.015.64775616
GGTATCA6750.015.3341351
CCGACCA2050.015.294179
CGGACAT1350.014.7792155
TCCAACG2250.014.35316118
TGTGCGA400.00527368414.25138610
CCAACGA1400.014.24837619
GTTTTCG759.65063E-713.93468915
CGAACGA759.656378E-713.93395316
AGAACCG551.9572629E-413.8195275
GCGTCCC903.630703E-813.72283316
GAACGCC1051.366061E-913.57274914
CCCCCGT852.7059468E-713.4074062
CGGTTCT2700.013.37167112
AACCGCG500.001497694913.3012937
GAACCGC500.001497694913.3012936
CATCGCC1650.013.24371115
ATCGCCA1650.013.24301216
Produced by FastQC (version 0.11.5)