##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062064_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	948404
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318256776647928	32.0	32.0	32.0	32.0	32.0
2	30.91839026406468	32.0	32.0	32.0	32.0	32.0
3	30.934874800190634	32.0	32.0	32.0	32.0	32.0
4	30.930623447391618	32.0	32.0	32.0	32.0	32.0
5	30.908602241238967	32.0	32.0	32.0	32.0	32.0
6	34.58003867550116	36.0	36.0	36.0	32.0	36.0
7	34.485097068337964	36.0	36.0	36.0	32.0	36.0
8	34.46981033399268	36.0	36.0	36.0	32.0	36.0
9	34.54663624362613	36.0	36.0	36.0	32.0	36.0
10	34.268904390955754	36.0	36.0	36.0	32.0	36.0
11	34.524140556134306	36.0	36.0	36.0	32.0	36.0
12	34.34096439913792	36.0	36.0	36.0	32.0	36.0
13	34.43892370761827	36.0	36.0	36.0	32.0	36.0
14	34.36894719971658	36.0	36.0	36.0	32.0	36.0
15	34.337373102601845	36.0	36.0	36.0	32.0	36.0
16	34.33410656218236	36.0	36.0	36.0	32.0	36.0
17	34.26282048578454	36.0	36.0	36.0	32.0	36.0
18	34.279067781240904	36.0	36.0	36.0	32.0	36.0
19	34.217684657593175	36.0	36.0	36.0	32.0	36.0
20	34.19918410297721	36.0	36.0	36.0	32.0	36.0
21	34.161832931957264	36.0	36.0	36.0	32.0	36.0
22	34.09137139868663	36.0	36.0	36.0	32.0	36.0
23	34.08573666918318	36.0	36.0	36.0	32.0	36.0
24	34.041830274861766	36.0	36.0	36.0	32.0	36.0
25	33.70666930970346	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	12.0
5	69.0
6	183.0
7	25.0
8	164.0
9	129.0
10	79.0
11	21.0
12	32.0
13	48.0
14	101.0
15	178.0
16	269.0
17	403.0
18	493.0
19	671.0
20	1049.0
21	1567.0
22	2299.0
23	3592.0
24	5073.0
25	7265.0
26	9989.0
27	12900.0
28	17098.0
29	23080.0
30	30745.0
31	41699.0
32	59424.0
33	85984.0
34	196382.0
35	447381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86493046658927	20.14850383754414	12.315780483407195	24.670785212459393
2	13.411506934822997	20.770728024614034	44.43579832180868	21.381966718754285
3	18.85172438517244	25.83572684827857	29.43833588501006	25.874212881538934
4	10.754813081500494	17.123255168981157	39.42587849216652	32.696053257351835
5	11.767628132445806	39.94015405531689	35.44770019227909	12.844517619958213
6	30.81392343230563	39.04811038982277	18.281969233236374	11.85599694463523
7	27.91074220007536	31.36129649899252	22.156335199115908	18.571626101816207
8	24.157942625044594	40.014693122731906	20.051573705221337	15.775790547002162
9	26.329329597733302	14.738800061650068	20.952683572579232	37.979186768037394
10	15.977933801404212	28.380509950905346	32.86987277622341	22.771683471467032
11	35.25160257594748	21.524830820140878	25.881113202945087	17.342453400966562
12	24.54122459063645	24.713743782604702	32.10050034788907	18.644531278869778
13	29.659108544932238	21.996361881897354	26.25716195115462	22.087367622015787
14	20.025292483930933	24.629981305096393	26.573259004113726	28.771467206858954
15	21.6164196989933	35.3080541199698	22.27998585236841	20.795540328668487
16	20.709788703847583	27.93002398405567	31.035811404249568	20.324375907847177
17	19.853217517619388	30.803207594177685	28.831820888211375	20.511753999991555
18	20.576102583373267	26.75101280814384	33.77533075917861	18.897553849304284
19	24.29130484340106	25.96990446599319	26.847356667328043	22.891434023277707
20	24.313391504037757	27.40365740916632	28.098286539265228	20.1846645475307
21	23.619704739179944	26.532949051731325	25.782924515259577	24.064421693829157
22	23.614661547002612	30.172630343508633	26.879713929271798	19.332994180216957
23	20.16546105914072	29.675356537980598	29.153014546433038	21.00616785644565
24	23.2812251010206	27.068877634476912	29.86885526167211	19.78104200283037
25	21.32724078070504	28.644002511595023	29.40128006922716	20.627476638472782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	290.0
2	311.0
3	332.0
4	332.0
5	332.0
6	732.0
7	1132.0
8	1132.0
9	1132.0
10	1477.5
11	1823.0
12	1823.0
13	1823.0
14	2945.0
15	4067.0
16	4067.0
17	4067.0
18	7302.0
19	10537.0
20	10537.0
21	10537.0
22	18495.0
23	26453.0
24	26453.0
25	26453.0
26	41665.5
27	56878.0
28	56878.0
29	56878.0
30	75699.5
31	94521.0
32	94521.0
33	94521.0
34	104246.0
35	113971.0
36	113971.0
37	113971.0
38	115653.5
39	117336.0
40	117336.0
41	117336.0
42	117796.5
43	118257.0
44	118257.0
45	118257.0
46	117156.0
47	116055.0
48	116055.0
49	116055.0
50	104631.5
51	93208.0
52	93208.0
53	93208.0
54	74496.0
55	55784.0
56	55784.0
57	55784.0
58	49571.5
59	43359.0
60	43359.0
61	43359.0
62	39602.0
63	35845.0
64	35845.0
65	35845.0
66	30608.0
67	25371.0
68	25371.0
69	25371.0
70	19112.0
71	12853.0
72	12853.0
73	12853.0
74	10790.5
75	8728.0
76	8728.0
77	8728.0
78	7690.5
79	6653.0
80	6653.0
81	6653.0
82	4622.5
83	2592.0
84	2592.0
85	2592.0
86	1992.0
87	1392.0
88	1392.0
89	1392.0
90	940.5
91	489.0
92	489.0
93	489.0
94	300.5
95	112.0
96	112.0
97	112.0
98	239.0
99	366.0
100	366.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011598432735416553
2	2.1088059518939185E-4
3	8.435223807575674E-4
4	0.010860350652253681
5	0.03194841017119287
6	0.05936288754581381
7	0.10449133491634366
8	0.10775998414177924
9	0.11830401390124884
10	0.1322221331837487
11	0.14076279728891908
12	0.13317109586210096
13	0.12768820038717676
14	0.11461360348543448
15	0.1316949316957752
16	0.11682784973492308
17	0.12146722282908973
18	0.10944702890329439
19	0.09363098426408999
20	0.08941337236030215
21	0.0880426484915711
22	0.09974652152458234
23	0.07897478289842726
24	0.08698824551562415
25	0.08424679777816205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	948404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.84604926771441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95981284962727	38.2576114504049
2	12.745771481246523	12.19669620493501
3	3.3499956150625563	4.808521657347311
4	1.2373837035216289	2.368156865670511
5	0.6126668060666336	1.4656843093878689
6	0.3718896712424423	1.0676070919452008
7	0.23904511953197893	0.8006155196433622
8	0.16716770105752307	0.6398651248615044
9	0.1291376779240657	0.5560854930245775
>10	0.894402306530069	8.630995229172314
>50	0.13160592775614707	4.416685641568372
>100	0.13804042610617528	13.58240494514256
>500	0.01597895607255859	5.270584317224753
>1k	0.007101758254470484	5.93848614967184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	4678	0.4932497121479876	No Hit
GTCCTACAGTGGACATTTCTAAATT	4656	0.4909300256009042	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4053	0.4273495261513026	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3376	0.35596644467969346	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3172	0.3344566239703755	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2443	0.25759064702384216	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1761	0.18568036406425953	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1663	0.17534721489997931	No Hit
GATATACACTGTTCTACAAATCCCG	1595	0.16817727466354002	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1553	0.1637487821645628	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1508	0.15900396877280146	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1467	0.1546809165714189	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1421	0.14983066288206293	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1408	0.14845993901333188	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1389	0.14645657335903264	No Hit
TTGTAGAACAGTGTATATCAATGAG	1293	0.13633430478994182	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1271	0.1340146182428585	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1246	0.13137861080299112	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1242	0.13095684961261234	No Hit
TCGTAGTTCCGACCATAAACGATGC	1230	0.129691566041476	No Hit
GAATATGGCAAGAAAACTGAAAATC	1205	0.1270555586016086	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1198	0.12631747651844571	No Hit
GTGTATATCAATGAGTTACAATGAA	1170	0.12336514818579424	No Hit
GAACTACGACGGTATCTGATCGTCT	1158	0.12209986461465788	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1155	0.1217835437218738	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1151	0.12136178253149502	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1123	0.11840945419884354	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1094	0.11535168556859735	No Hit
CTGTAGGACATGGAATATGGCAAGA	1089	0.11482448408062387	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1075	0.11334831991429813	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1049	0.11060687217683604	No Hit
GTGTATATCAATGAGTTACAATGAG	1034	0.1090252677129156	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	995	0.10491309610672246	No Hit
GCCATGCACCACCACCCACGGAATC	964	0.10164444688128688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGTC	40	2.757641E-4	16.626617	10
CGACCAT	200	0.0	15.676524	10
AGCCCCG	170	0.0	15.647756	16
GGTATCA	675	0.0	15.334135	1
CCGACCA	205	0.0	15.29417	9
CGGACAT	135	0.0	14.779215	5
TCCAACG	225	0.0	14.353161	18
TGTGCGA	40	0.005273684	14.251386	10
CCAACGA	140	0.0	14.248376	19
GTTTTCG	75	9.65063E-7	13.934689	15
CGAACGA	75	9.656378E-7	13.933953	16
AGAACCG	55	1.9572629E-4	13.819527	5
GCGTCCC	90	3.630703E-8	13.722833	16
GAACGCC	105	1.366061E-9	13.572749	14
CCCCCGT	85	2.7059468E-7	13.407406	2
CGGTTCT	270	0.0	13.371671	12
AACCGCG	50	0.0014976949	13.301293	7
GAACCGC	50	0.0014976949	13.301293	6
CATCGCC	165	0.0	13.243711	15
ATCGCCA	165	0.0	13.243012	16
>>END_MODULE