FastQCFastQC Report
Thu 2 Feb 2017
SRR4062064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948404
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA24440.25769608732143684No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC16830.17745602085187326No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16810.17724514025668386No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA14500.1528884315123091No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14260.1503578643700364No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13710.14455864800232812No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13480.14213352115765013No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13470.14202808086005542No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA13300.1402355958009456No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13180.13897031222980924No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT13000.13707238687310472No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG12120.12779364068477148No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA12040.1269501183040139No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA11500.12125634223390032No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11200.11809313330605944No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC10830.11419184229505569No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT10390.10955246920088908No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG9700.10227708866685506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTA250.002352026335.2015636
GGTATCA7300.028.9434721
GTATCAA18000.026.9000111
CGATAAA508.343035E-526.39838821
GGACCTG17650.026.04946
AGGACCT18550.025.8528695
TACTCGC609.746735E-625.66780544
CGGTAGG450.001394628324.4545541
CTGTAGG29650.024.3473321
TGTAGGA31100.024.3342022
GTAGGAC31750.023.696193
TAGGACC16800.023.5699884
GACCTGG19450.022.6207247
GTCCTAC24900.022.2742671
CGTAAAC602.8702614E-422.00213440
CGCGGGA2200.022.00097744
TCCTACA26500.021.9998172
TAGGACG30850.021.6064594
CCTACAG26350.021.539483
CGCTTCG2250.021.51206432
Produced by FastQC (version 0.11.5)