##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062064_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	948404
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.281183968013632	32.0	32.0	32.0	32.0	32.0
2	31.509922986406636	32.0	32.0	32.0	32.0	32.0
3	31.627425654046167	32.0	32.0	32.0	32.0	32.0
4	31.695730933230987	32.0	32.0	32.0	32.0	32.0
5	31.68482418884779	32.0	32.0	32.0	32.0	32.0
6	35.34886925824859	36.0	36.0	36.0	36.0	36.0
7	35.343967338813414	36.0	36.0	36.0	36.0	36.0
8	35.30695463114875	36.0	36.0	36.0	36.0	36.0
9	35.38033686066276	36.0	36.0	36.0	36.0	36.0
10	35.2772573713312	36.0	36.0	36.0	36.0	36.0
11	35.37547395413769	36.0	36.0	36.0	36.0	36.0
12	35.307848764872354	36.0	36.0	36.0	36.0	36.0
13	35.33329467189088	36.0	36.0	36.0	36.0	36.0
14	35.31098350491985	36.0	36.0	36.0	36.0	36.0
15	35.29259682582528	36.0	36.0	36.0	36.0	36.0
16	35.30404553333811	36.0	36.0	36.0	36.0	36.0
17	35.285386818275754	36.0	36.0	36.0	36.0	36.0
18	35.28283938068587	36.0	36.0	36.0	36.0	36.0
19	35.25012020193926	36.0	36.0	36.0	36.0	36.0
20	35.242486324393404	36.0	36.0	36.0	36.0	36.0
21	35.214189311727914	36.0	36.0	36.0	36.0	36.0
22	35.16797061168025	36.0	36.0	36.0	36.0	36.0
23	35.16719351668698	36.0	36.0	36.0	36.0	36.0
24	35.14010590423491	36.0	36.0	36.0	36.0	36.0
25	35.09203356375553	36.0	36.0	36.0	36.0	36.0
26	35.07464224107026	36.0	36.0	36.0	36.0	36.0
27	35.027291112226436	36.0	36.0	36.0	36.0	36.0
28	34.95913028624932	36.0	36.0	36.0	36.0	36.0
29	34.92320361364988	36.0	36.0	36.0	36.0	36.0
30	34.86334410230239	36.0	36.0	36.0	32.0	36.0
31	34.87385649997258	36.0	36.0	36.0	36.0	36.0
32	34.837000898351334	36.0	36.0	36.0	32.0	36.0
33	34.86786643666623	36.0	36.0	36.0	36.0	36.0
34	34.842695728824424	36.0	36.0	36.0	36.0	36.0
35	34.82961375110185	36.0	36.0	36.0	36.0	36.0
36	34.759777478795954	36.0	36.0	36.0	32.0	36.0
37	34.65702485438695	36.0	36.0	36.0	32.0	36.0
38	34.62685943964808	36.0	36.0	36.0	32.0	36.0
39	34.44829313246254	36.0	36.0	36.0	32.0	36.0
40	34.53925015077962	36.0	36.0	36.0	32.0	36.0
41	34.525875049029736	36.0	36.0	36.0	32.0	36.0
42	34.49648145726926	36.0	36.0	36.0	32.0	36.0
43	34.53362912851485	36.0	36.0	36.0	32.0	36.0
44	34.401182407497224	36.0	36.0	36.0	32.0	36.0
45	34.38084930050906	36.0	36.0	36.0	32.0	36.0
46	34.227549651836135	36.0	36.0	36.0	32.0	36.0
47	34.23949287434469	36.0	36.0	36.0	32.0	36.0
48	34.19655969397008	36.0	36.0	36.0	32.0	36.0
49	34.14346312331032	36.0	36.0	36.0	32.0	36.0
50	33.818175587618775	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	19.0
22	53.0
23	169.0
24	413.0
25	1053.0
26	2312.0
27	4653.0
28	8210.0
29	13098.0
30	19655.0
31	29106.0
32	43687.0
33	73750.0
34	163485.0
35	588731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43254634590882	20.745753558741384	12.72561196850925	25.09608812684055
2	12.994294282578	21.180432900615493	44.194582784571466	21.630690032235037
3	18.45810110058815	26.178455971202087	29.722733608743972	25.6407093194658
4	10.628909199033323	17.440457863948275	39.691523865356956	32.239109071661446
5	11.447336788963353	40.34630811342002	35.14609807634721	13.060257021269416
6	30.879699874420474	39.17912532963308	18.169174918257323	11.771999877689126
7	27.885373743678855	31.53086659271787	22.265405881881563	18.318353781721715
8	24.260336312373205	40.10284646627387	19.86980232053007	15.767014900822856
9	26.30524544392474	14.576383060383549	21.046726922282065	38.07164457340965
10	15.967351466252778	28.53636214102851	32.72423988089464	22.772046511824076
11	35.24057258299206	21.4714404409935	26.041012058152436	17.24697491786201
12	24.678617972931367	24.562949966469986	32.08263567003091	18.675796390567733
13	29.73806521271526	21.8928853104795	26.32433013778938	22.044719339015863
14	20.068451841198474	24.620731249551877	26.612498471115686	28.698318438133956
15	21.65374671553473	35.39788950700334	22.218274068856733	20.730089708605192
16	20.843552363258308	27.85424623099682	30.93081567365462	20.37138573209025
17	19.928005364802342	31.025596686644086	28.515379521807162	20.53101842674641
18	20.76661584433605	26.739610418366894	33.626352538269636	18.86742119902742
19	24.41079961704084	25.919123074132965	26.975634856031817	22.694442452794377
20	24.559574019401094	27.287853226486714	27.97543230704344	20.177140447068748
21	23.794606517897435	26.34562907790351	25.82538664957128	24.034377754627776
22	23.69074782476666	29.960544240640065	27.03605214655358	19.312655788039695
23	20.272900578234594	29.556391579959595	29.177122829511475	20.99358501229434
24	23.539558939049655	26.964081421770462	29.70323546623506	19.793124172944818
25	21.537115141290595	28.473428932939687	29.2909110080135	20.69854491775622
26	18.930594393059437	30.828156171050914	30.515206727991263	19.72604270789839
27	20.662927734002253	29.064771261884502	29.17843650193642	21.093864502176825
28	19.66536726196062	29.890982862408965	27.85267133636938	22.590978539261037
29	20.93893268212856	27.782487484131103	27.679998650352804	23.59858118338753
30	19.630868767984445	28.350708411843662	29.845980921330806	22.17244189884109
31	23.53954027836356	27.003268663011387	27.853964571910588	21.603226486714465
32	21.092754894600546	29.216716294528	28.124446431433412	21.56608237943804
33	19.658961384314797	29.437883080608646	30.338138986099704	20.565016548976853
34	21.35382123730885	29.458233208245073	30.034679569842588	19.153265984603493
35	21.13407605635585	29.635639739266683	29.762168363204633	19.468115841172835
36	18.92697513609721	29.42209166356496	30.647941855940985	21.002991344396843
37	20.697479718173824	28.790387036502015	27.706967416220824	22.80516582910334
38	20.860097595539454	30.123660381019057	27.740498774783738	21.275743248657744
39	23.423468069738878	27.04274063022264	28.024820881594692	21.50897041844379
40	24.464335233724974	27.143897992515825	29.069771120022015	19.321995653737183
41	22.880647327948072	26.88848797595085	30.62671408733318	19.604150608767902
42	22.404993884179003	30.472921675313174	28.856341473701967	18.265742966805853
43	20.214284810473615	29.642626670771982	29.899384624469082	20.243703894285318
44	19.35798844957047	30.235890687426647	28.6000191909638	21.806101672039084
45	19.691368438811445	30.658069550180304	28.423100444969318	21.22746156603893
46	20.337081495103206	27.746695403510845	28.953284351354313	22.962938750031633
47	23.041210807345486	27.340698782781804	28.786303553804753	20.831786856067954
48	21.331126037306113	29.03181247825216	27.29862817254869	22.338433311893038
49	22.299676918664485	28.645314898857826	27.30785939635247	21.747148786125216
50	20.645915028717056	31.205589255908166	26.680873184161385	21.467622531213394
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	67.5
2	24.0
3	56.0
4	88.0
5	97.5
6	107.0
7	98.0
8	89.0
9	123.5
10	158.0
11	282.0
12	406.0
13	749.0
14	1092.0
15	1464.5
16	1837.0
17	2087.0
18	2337.0
19	2638.0
20	2939.0
21	3632.5
22	4326.0
23	5683.5
24	7041.0
25	9539.5
26	12038.0
27	17460.5
28	22883.0
29	28619.0
30	34355.0
31	41328.5
32	48302.0
33	58458.5
34	68615.0
35	79408.0
36	90201.0
37	91666.0
38	93131.0
39	86663.0
40	80195.0
41	72968.5
42	65742.0
43	58949.5
44	52157.0
45	50700.0
46	49243.0
47	48393.5
48	47544.0
49	46488.0
50	45432.0
51	38556.5
52	31681.0
53	29755.0
54	27829.0
55	29970.5
56	32112.0
57	31259.0
58	30406.0
59	27502.5
60	24599.0
61	21298.5
62	17998.0
63	15239.5
64	12481.0
65	10734.0
66	8987.0
67	8774.5
68	8562.0
69	8625.0
70	8688.0
71	6579.0
72	4470.0
73	4317.0
74	4164.0
75	2829.0
76	1494.0
77	1517.5
78	1541.0
79	1346.5
80	1152.0
81	904.0
82	656.0
83	632.0
84	608.0
85	484.0
86	360.0
87	249.0
88	138.0
89	89.5
90	41.0
91	30.0
92	19.0
93	14.5
94	10.0
95	8.5
96	7.0
97	4.5
98	2.0
99	4.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03331913403992392
2	0.006010096962897668
3	2.1088059518939185E-4
4	0.0
5	0.0
6	1.0544029759469592E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010544029759469594
17	0.0
18	2.1088059518939185E-4
19	0.0
20	4.217611903787837E-4
21	0.0
22	0.0
23	0.0
24	9.489626783522635E-4
25	4.217611903787837E-4
26	8.435223807575674E-4
27	5.272014879734797E-4
28	0.0022142462494886142
29	8.435223807575674E-4
30	0.001581604463920439
31	4.217611903787837E-4
32	0.0012652835711363512
33	7.380820831628715E-4
34	7.380820831628715E-4
35	2.1088059518939185E-4
36	1.0544029759469592E-4
37	0.001897925356704527
38	0.0
39	9.489626783522635E-4
40	3.163208927840878E-4
41	0.001370723868731047
42	0.004639373094166621
43	0.0042176119037878376
44	0.004323052201382533
45	0.0025305671422727024
46	0.002952328332651486
47	0.0012652835711363512
48	0.0035849701182196617
49	0.003795850713409054
50	0.001370723868731047
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	948404.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.4710625825804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.85852757424664	45.66166970980585
2	12.685770044747041	14.327578282102676
3	3.223585633011043	5.461179180662209
4	1.1521156377383774	2.6024477712437375
5	0.5288578939854109	1.4932583614270905
6	0.3068750819564764	1.03977371749192
7	0.18835771218328262	0.7445732106829672
8	0.13263279933419872	0.5991932089363491
9	0.0996685891096982	0.5065552019818131
>10	0.6236545838457863	6.9773014759435075
>50	0.09725281654488428	3.826697251173276
>100	0.0899109537680832	10.527994995912684
>500	0.009593009646846626	3.7349452021331158
>1k	0.0031976698822822084	2.4968324305028444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2444	0.25769608732143684	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1683	0.17745602085187326	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1681	0.17724514025668386	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1450	0.1528884315123091	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1426	0.1503578643700364	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1371	0.14455864800232812	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1348	0.14213352115765013	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1347	0.14202808086005542	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1330	0.1402355958009456	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1318	0.13897031222980924	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1300	0.13707238687310472	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1212	0.12779364068477148	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1204	0.1269501183040139	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1150	0.12125634223390032	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1120	0.11809313330605944	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1083	0.11419184229505569	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	1039	0.10955246920088908	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	970	0.10227708866685506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.0544029759469594E-4	0.0
4	0.0	0.0	0.0	1.0544029759469594E-4	0.0
5	0.0	0.0	0.0	1.0544029759469594E-4	0.0
6	0.0	0.0	0.0	1.0544029759469594E-4	0.0
7	0.0	0.0	0.0	1.0544029759469594E-4	0.0
8	0.0	0.0	0.0	1.0544029759469594E-4	0.0
9	0.0	0.0	0.0	1.0544029759469594E-4	0.0
10	0.0	0.0	0.0	1.0544029759469594E-4	0.0
11	0.0	0.0	0.0	1.0544029759469594E-4	0.0
12	1.0544029759469594E-4	0.0	0.0	1.0544029759469594E-4	0.0
13	1.0544029759469594E-4	0.0	0.0	1.0544029759469594E-4	0.0
14	1.0544029759469594E-4	0.0	0.0	1.0544029759469594E-4	0.0
15	1.0544029759469594E-4	0.0	0.0	1.0544029759469594E-4	0.0
16	1.0544029759469594E-4	0.0	0.0	2.1088059518939187E-4	0.0
17	1.0544029759469594E-4	0.0	0.0	2.1088059518939187E-4	0.0
18	1.0544029759469594E-4	0.0	0.0	2.1088059518939187E-4	0.0
19	1.0544029759469594E-4	0.0	0.0	2.1088059518939187E-4	0.0
20	1.0544029759469594E-4	0.0	0.0	2.1088059518939187E-4	0.0
21	1.0544029759469594E-4	0.0	0.0	3.163208927840878E-4	0.0
22	1.0544029759469594E-4	0.0	0.0	4.2176119037878374E-4	0.0
23	1.0544029759469594E-4	0.0	0.0	6.326417855681756E-4	0.0
24	1.0544029759469594E-4	0.0	0.0	8.435223807575675E-4	0.0
25	1.0544029759469594E-4	0.0	0.0	0.0011598432735416553	0.0
26	1.0544029759469594E-4	0.0	0.0	0.001687044761515135	0.0
27	1.0544029759469594E-4	0.0	0.0	0.0021088059518939188	0.0
28	1.0544029759469594E-4	0.0	0.0	0.003268649225435574	0.0
29	1.0544029759469594E-4	0.0	0.0	0.0039012910110037494	0.0
30	1.0544029759469594E-4	0.0	0.0	0.006010096962897668	0.0
31	1.0544029759469594E-4	0.0	0.0	0.008435223807575675	0.0
32	1.0544029759469594E-4	0.0	0.0	0.012758276008958208	0.0
33	1.0544029759469594E-4	0.0	0.0	0.01739764910312483	0.0
34	1.0544029759469594E-4	0.0	0.0	0.023407746066022497	0.0
35	1.0544029759469594E-4	0.0	0.0	0.028574320648162597	0.0
36	1.0544029759469594E-4	0.0	0.0	0.03658778326535949	0.0
37	1.0544029759469594E-4	0.0	0.0	0.05050590254785935	0.0
38	1.0544029759469594E-4	0.0	0.0	0.06663826807984782	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAGTA	25	0.0023520263	35.20156	36
GGTATCA	730	0.0	28.943472	1
GTATCAA	1800	0.0	26.900011	1
CGATAAA	50	8.343035E-5	26.398388	21
GGACCTG	1765	0.0	26.0494	6
AGGACCT	1855	0.0	25.852869	5
TACTCGC	60	9.746735E-6	25.667805	44
CGGTAGG	45	0.0013946283	24.454554	1
CTGTAGG	2965	0.0	24.347332	1
TGTAGGA	3110	0.0	24.334202	2
GTAGGAC	3175	0.0	23.69619	3
TAGGACC	1680	0.0	23.569988	4
GACCTGG	1945	0.0	22.620724	7
GTCCTAC	2490	0.0	22.274267	1
CGTAAAC	60	2.8702614E-4	22.002134	40
CGCGGGA	220	0.0	22.000977	44
TCCTACA	2650	0.0	21.999817	2
TAGGACG	3085	0.0	21.606459	4
CCTACAG	2635	0.0	21.53948	3
CGCTTCG	225	0.0	21.512064	32
>>END_MODULE