FastQCFastQC Report
Thu 2 Feb 2017
SRR4062063_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062063_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307944
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12270.3984490686618346No Hit
TATCAACGCAGAGTACTTTTTTTTT8140.26433377497207283No Hit
GGTATCAACGCAGAGTACTTTTTTT5290.17178448029511859No Hit
GCGCAAGACGGACCAGAGCGAAAGC4120.13379055932247422No Hit
ATCAGATACCGTCGTAGTTCCGACC3670.11917751279453406No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3500.11365702855064556No Hit
ACCATACTCCCCCCGGAACCCAAAG3260.10586340373574417No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAT307.697292E-419.00227712
TAGCAAT250.0060171619.00227518
TTTTCGG250.006022814718.99918616
TCTACAC250.006028473418.99612
CCTACAC453.5302335E-516.8854223
GGTATCA2250.016.460611
GGTAATA350.002163149916.28766413
GCCGCGC350.002163149916.2876648
TCGGTTT702.476554E-816.28766413
GTCGGTT702.476554E-816.28766412
GGTTTGA702.4814653E-816.28501715
CTACACG350.002165501216.2850174
AATAACC702.4865585E-816.282372
CGGTTTG755.7962097E-815.1993514
CTGGTCG704.3964974E-714.9303599
TTTATAT456.751047E-414.7747443
TCCATTA400.005262189514.2517088
CGCGCCA400.005262189514.25170810
GAGGTAA400.005262189514.25170811
GGACTGT400.0052678714.2493916
Produced by FastQC (version 0.11.5)