##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062063_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307944
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24385602577092	32.0	32.0	32.0	32.0	32.0
2	30.866524433013794	32.0	32.0	32.0	32.0	32.0
3	30.87114865040397	32.0	32.0	32.0	32.0	32.0
4	30.877773231496636	32.0	32.0	32.0	32.0	32.0
5	30.836499493414387	32.0	32.0	32.0	32.0	32.0
6	34.46993609227652	36.0	36.0	36.0	32.0	36.0
7	34.3738634297145	36.0	36.0	36.0	32.0	36.0
8	34.34568298132128	36.0	36.0	36.0	32.0	36.0
9	34.44169394435352	36.0	36.0	36.0	32.0	36.0
10	34.21485075208479	36.0	36.0	36.0	32.0	36.0
11	34.44138219936092	36.0	36.0	36.0	32.0	36.0
12	34.284999870106255	36.0	36.0	36.0	32.0	36.0
13	34.37674707089601	36.0	36.0	36.0	32.0	36.0
14	34.27776478840309	36.0	36.0	36.0	32.0	36.0
15	34.22939235705193	36.0	36.0	36.0	32.0	36.0
16	34.24520367339516	36.0	36.0	36.0	32.0	36.0
17	34.18099069960772	36.0	36.0	36.0	32.0	36.0
18	34.18617021276596	36.0	36.0	36.0	32.0	36.0
19	34.17861689138285	36.0	36.0	36.0	32.0	36.0
20	34.17508702881043	36.0	36.0	36.0	32.0	36.0
21	34.167147273530254	36.0	36.0	36.0	32.0	36.0
22	34.11228989686437	36.0	36.0	36.0	32.0	36.0
23	34.10030395136778	36.0	36.0	36.0	32.0	36.0
24	34.07714389629283	36.0	36.0	36.0	32.0	36.0
25	33.76161899566155	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	17.0
6	71.0
7	17.0
8	55.0
9	46.0
10	21.0
11	4.0
12	18.0
13	10.0
14	83.0
15	125.0
16	164.0
17	223.0
18	242.0
19	308.0
20	458.0
21	653.0
22	894.0
23	1224.0
24	1780.0
25	2355.0
26	3178.0
27	4200.0
28	5548.0
29	7397.0
30	9996.0
31	13616.0
32	19600.0
33	28437.0
34	64419.0
35	142780.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45322759776319	18.984665744403095	12.981834005546572	24.58027265228715
2	16.413427160221207	20.147235040250955	38.05899143672693	25.38034636280091
3	18.762035584738634	24.65115070744071	27.89527864103838	28.691535066782276
4	12.952546732310571	16.292656629557943	35.413608552759776	35.34118808537172
5	14.364511665074941	36.80232067099356	33.724767900416445	15.108399763515049
6	32.976988113883166	36.09018891712212	16.875166529110913	14.057656439883804
7	30.27717094483889	30.246937660919095	20.583014746040416	18.892876648201607
8	27.841982243807927	32.71794571715576	19.326682075738514	20.113389963297802
9	26.983776050980264	14.957245505088274	19.924244887342716	38.13473355658875
10	17.442344463377516	26.628207963162893	29.79487246192068	26.13457511153891
11	35.60989411244374	22.81883341571923	22.122238988474646	19.449033483362385
12	24.127368154059276	24.868462984280594	27.845849673840227	23.158319187819902
13	28.980149246451738	20.40351818433075	24.91830463834561	25.698027930871902
14	23.821011597246834	19.632737594002077	25.42583386708196	31.12041694166913
15	25.301616207603004	27.83486715879158	21.98985398848818	24.873662645117232
16	25.486562233796974	26.049368265673056	23.92917292548591	24.534896575044055
17	23.025573493309924	27.531255588613046	24.95407176185599	24.48909915622104
18	23.879641068990182	26.256258534365045	26.52545679172898	23.33864360491579
19	25.317184010610184	25.435833422726727	26.045333827434998	23.201648739228094
20	25.849964246245854	24.680166417473835	25.702073717740365	23.767795618539946
21	26.352348447911588	24.859418169998374	24.84219080123517	23.94604258085487
22	25.864305288719414	24.778864627117485	25.763855703683475	23.59297438047962
23	24.414160258452018	25.277075384005354	26.141290570011506	24.167473787531122
24	24.46415685560677	25.240373676238274	25.93695351150348	24.358515956651473
25	24.490499067133857	24.85259414797142	26.395886287842835	24.261020497051884
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	108.0
2	133.0
3	158.0
4	158.0
5	158.0
6	295.5
7	433.0
8	433.0
9	433.0
10	484.0
11	535.0
12	535.0
13	535.0
14	651.0
15	767.0
16	767.0
17	767.0
18	1897.5
19	3028.0
20	3028.0
21	3028.0
22	4099.0
23	5170.0
24	5170.0
25	5170.0
26	7975.5
27	10781.0
28	10781.0
29	10781.0
30	14010.5
31	17240.0
32	17240.0
33	17240.0
34	19917.0
35	22594.0
36	22594.0
37	22594.0
38	24316.0
39	26038.0
40	26038.0
41	26038.0
42	28442.5
43	30847.0
44	30847.0
45	30847.0
46	34081.0
47	37315.0
48	37315.0
49	37315.0
50	38015.5
51	38716.0
52	38716.0
53	38716.0
54	36543.5
55	34371.0
56	34371.0
57	34371.0
58	31286.0
59	28201.0
60	28201.0
61	28201.0
62	24864.0
63	21527.0
64	21527.0
65	21527.0
66	17748.5
67	13970.0
68	13970.0
69	13970.0
70	10813.0
71	7656.0
72	7656.0
73	7656.0
74	6116.0
75	4576.0
76	4576.0
77	4576.0
78	3404.5
79	2233.0
80	2233.0
81	2233.0
82	1593.5
83	954.0
84	954.0
85	954.0
86	708.0
87	462.0
88	462.0
89	462.0
90	305.0
91	148.0
92	148.0
93	148.0
94	83.5
95	19.0
96	19.0
97	19.0
98	58.0
99	97.0
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0019484062037253527
2	3.247343672875588E-4
3	9.742031018626764E-4
4	0.006494687345751176
5	0.033122905463331
6	0.061699529784636165
7	0.10911074740861974
8	0.10813654430675707
9	0.12145065336554699
10	0.13898630919907515
11	0.14548099654482632
12	0.13833684046450004
13	0.12956901254773595
14	0.12047645026368431
15	0.1409347154028005
16	0.12274959083469722
17	0.12956901254773595
18	0.1182033096926714
19	0.10229132569558103
20	0.09222456030966669
21	0.09547190398254228
22	0.10618813810303174
23	0.08637934169849062
24	0.09677084145169251
25	0.09417296651339205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	307944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.469176213857065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.44417444686021	17.807458498947863
2	14.321950803598074	8.72756085522043
3	7.482840943002089	6.83987997817785
4	4.550880334228589	5.546462993271504
5	3.105682738628128	4.7313797313797314
6	2.1475465745832802	3.926038500506586
7	1.6839323016583536	3.5915621022004
8	1.2299100481732532	2.9979476787987425
9	0.9719913032357079	2.6654196866962825
>10	5.6550283497463445	30.85171329852181
>50	0.2920237029458157	6.020899903878627
>100	0.11084111352687898	5.459109448471151
>500	0.0021315598755169032	0.43611825526719145
>1k	0.0010657799377584516	0.3984490686618346
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1227	0.3984490686618346	No Hit
TATCAACGCAGAGTACTTTTTTTTT	814	0.26433377497207283	No Hit
GGTATCAACGCAGAGTACTTTTTTT	529	0.17178448029511859	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	412	0.13379055932247422	No Hit
ATCAGATACCGTCGTAGTTCCGACC	367	0.11917751279453406	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	350	0.11365702855064556	No Hit
ACCATACTCCCCCCGGAACCCAAAG	326	0.10586340373574417	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.247343672875588E-4	0.0
8	0.0	0.0	0.0	3.247343672875588E-4	0.0
9	0.0	0.0	0.0	3.247343672875588E-4	0.0
10	0.0	0.0	0.0	3.247343672875588E-4	0.0
11	0.0	0.0	0.0	3.247343672875588E-4	0.0
12	0.0	0.0	0.0	3.247343672875588E-4	0.0
13	0.0	0.0	0.0	3.247343672875588E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAT	30	7.697292E-4	19.002277	12
TAGCAAT	25	0.00601716	19.002275	18
TTTTCGG	25	0.0060228147	18.999186	16
TCTACAC	25	0.0060284734	18.9961	2
CCTACAC	45	3.5302335E-5	16.885422	3
GGTATCA	225	0.0	16.46061	1
GGTAATA	35	0.0021631499	16.287664	13
GCCGCGC	35	0.0021631499	16.287664	8
TCGGTTT	70	2.476554E-8	16.287664	13
GTCGGTT	70	2.476554E-8	16.287664	12
GGTTTGA	70	2.4814653E-8	16.285017	15
CTACACG	35	0.0021655012	16.285017	4
AATAACC	70	2.4865585E-8	16.28237	2
CGGTTTG	75	5.7962097E-8	15.19935	14
CTGGTCG	70	4.3964974E-7	14.930359	9
TTTATAT	45	6.751047E-4	14.774744	3
TCCATTA	40	0.0052621895	14.251708	8
CGCGCCA	40	0.0052621895	14.251708	10
GAGGTAA	40	0.0052621895	14.251708	11
GGACTGT	40	0.00526787	14.249391	6
>>END_MODULE