FastQCFastQC Report
Thu 2 Feb 2017
SRR4062063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307944
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.2094536669004754No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT4470.14515626217753877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.1331410905878991No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG3420.1110591536123451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.1094354817759073No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA3240.10521393500116906No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG3240.10521393500116906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA357.2669573E-637.71874240
CGCTTAC401.8162424E-532.9985418
ACTTAAC353.2101115E-431.43228538
GGTATCA2700.030.9817161
ATTAGAA454.0565435E-529.33679841
ATAGCTA454.0565435E-529.33679840
GTTTAGG300.005739693529.33203525
TAGCCGA300.005739693529.3320359
GGGCGTT454.0610423E-529.33203533
GTATCAA6250.028.1770571
TAGCGGC554.947009E-627.99875830
TAAACGC407.021972E-427.49878328
AACGCTT407.021972E-427.49878330
GGTCGGT655.909369E-727.07572611
CGACGCT508.3241284E-526.39883215
ACGCTTA508.3241284E-526.39883217
GACGCTT508.3241284E-526.39883216
CGCAATA609.713294E-625.66969936
CTAGCGG609.725549E-625.6655329
GGTTTGA701.1281245E-625.14174515