##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307944
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.291832281193983	32.0	32.0	32.0	32.0	32.0
2	31.51139817629179	32.0	32.0	32.0	32.0	32.0
3	31.592526563271242	32.0	32.0	32.0	32.0	32.0
4	31.65678824721378	32.0	32.0	32.0	32.0	32.0
5	31.640509313381653	32.0	32.0	32.0	32.0	32.0
6	35.269779570311485	36.0	36.0	36.0	36.0	36.0
7	35.265950952121166	36.0	36.0	36.0	36.0	36.0
8	35.22734003585067	36.0	36.0	36.0	36.0	36.0
9	35.299573948510115	36.0	36.0	36.0	36.0	36.0
10	35.19293118229288	36.0	36.0	36.0	36.0	36.0
11	35.30294144389889	36.0	36.0	36.0	36.0	36.0
12	35.216364014236355	36.0	36.0	36.0	36.0	36.0
13	35.26680500350713	36.0	36.0	36.0	36.0	36.0
14	35.21829618372171	36.0	36.0	36.0	36.0	36.0
15	35.20851193723534	36.0	36.0	36.0	36.0	36.0
16	35.226674330397735	36.0	36.0	36.0	36.0	36.0
17	35.19649351830203	36.0	36.0	36.0	36.0	36.0
18	35.18679370275115	36.0	36.0	36.0	36.0	36.0
19	35.170355649079056	36.0	36.0	36.0	36.0	36.0
20	35.18954745018575	36.0	36.0	36.0	36.0	36.0
21	35.171508456082925	36.0	36.0	36.0	36.0	36.0
22	35.17097589172057	36.0	36.0	36.0	36.0	36.0
23	35.12533447639831	36.0	36.0	36.0	36.0	36.0
24	35.11958992544099	36.0	36.0	36.0	36.0	36.0
25	35.10775335775336	36.0	36.0	36.0	36.0	36.0
26	35.06717130387343	36.0	36.0	36.0	36.0	36.0
27	35.040556075130546	36.0	36.0	36.0	36.0	36.0
28	35.01608409321175	36.0	36.0	36.0	36.0	36.0
29	35.01459031512223	36.0	36.0	36.0	36.0	36.0
30	34.98203244745798	36.0	36.0	36.0	36.0	36.0
31	34.992596056425846	36.0	36.0	36.0	36.0	36.0
32	34.9599894786065	36.0	36.0	36.0	36.0	36.0
33	34.953757826098254	36.0	36.0	36.0	36.0	36.0
34	34.913659626425584	36.0	36.0	36.0	36.0	36.0
35	34.91148715350843	36.0	36.0	36.0	36.0	36.0
36	34.90652521237627	36.0	36.0	36.0	32.0	36.0
37	34.87866300366301	36.0	36.0	36.0	32.0	36.0
38	34.85250565037799	36.0	36.0	36.0	32.0	36.0
39	34.84644285974073	36.0	36.0	36.0	32.0	36.0
40	34.82315940560621	36.0	36.0	36.0	32.0	36.0
41	34.80672784662146	36.0	36.0	36.0	32.0	36.0
42	34.75877756994778	36.0	36.0	36.0	32.0	36.0
43	34.75756955810147	36.0	36.0	36.0	32.0	36.0
44	34.69643506611592	36.0	36.0	36.0	32.0	36.0
45	34.66125009742031	36.0	36.0	36.0	32.0	36.0
46	34.64120424492765	36.0	36.0	36.0	32.0	36.0
47	34.59031512223002	36.0	36.0	36.0	32.0	36.0
48	34.577286779414436	36.0	36.0	36.0	32.0	36.0
49	34.54959667991583	36.0	36.0	36.0	32.0	36.0
50	34.11615748317876	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	5.0
21	12.0
22	34.0
23	76.0
24	167.0
25	346.0
26	736.0
27	1380.0
28	2461.0
29	3877.0
30	5813.0
31	8542.0
32	13097.0
33	21513.0
34	48997.0
35	200886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1367038278631	19.44531067279956	13.235752709129182	25.182232790208154
2	15.806070289615038	20.426985704357538	37.720101582847825	26.046842423179594
3	18.06016048424546	25.034503138567853	28.159432102694332	28.745904274492357
4	12.659119839970904	16.625750136388433	35.646091497155325	35.069038526485336
5	14.079832696853972	36.966461434546545	33.555126906190736	15.398578962408749
6	33.08685994856207	36.064349362221705	16.717325227963524	14.131465461252695
7	30.274660327851816	30.31752526433377	20.68850180552308	18.71931260229133
8	27.829735276543786	32.801093705349025	19.34247785311615	20.026693164991038
9	27.295871976723042	14.507183124204401	19.75911204634609	38.43783285272647
10	17.566887378508362	26.267523535199693	29.970156814735194	26.19543227155675
11	35.815278102512146	22.509936871639	22.141038630400335	19.533746395448524
12	24.062816616008107	24.798989426649	27.90929519652924	23.228898760813653
13	29.139064245447223	20.26439872184553	24.97207284441327	25.624464188293977
14	23.94753591562102	19.526602239368195	25.405593224742162	31.12026862026862
15	25.46307120775206	27.53942275218871	22.136817083625594	24.860688956433638
16	25.62000110410043	25.913561925421263	24.028472801784744	24.437964168693558
17	23.239290260566854	27.009131530408126	24.999025796898135	24.752552412126878
18	23.97391725091981	26.023322497994762	26.545497056273405	23.45726319481203
19	25.31953861741096	25.088327747902216	26.307055828332427	23.2850778063544
20	25.80494315469522	24.368629055565837	25.9585440068065	23.867883782932445
21	26.42071285688307	24.529784636167616	25.104889200633885	23.944613306315436
22	26.031356350505284	24.54212454212454	25.878081729145556	23.548437378224612
23	24.47725715473318	25.103347047992646	26.152242460455344	24.26715333681883
24	24.320243163463132	25.077855823031037	26.39888809869423	24.2030129148116
25	24.47766137779192	24.592618090419624	26.613128446265854	24.316592085522597
26	23.808132077248565	25.703625045057333	26.41707340042411	24.071169477269997
27	24.672583384479495	25.418505492935335	25.807865792473233	24.101045330111937
28	24.512561051647094	24.396303127922685	26.670801725033773	24.420334095396445
29	23.91334703725714	25.397397553427442	26.552164213274622	24.1370911960408
30	24.020588426316817	25.423459115412093	26.491199584334613	24.06475287393648
31	24.75441724226394	24.866776427952107	26.25145725966987	24.12734907011408
32	24.691823082418654	24.962005585503668	25.563746184321623	24.782425147756058
33	24.3743160260178	24.975726027219324	26.116521564055684	24.533436382707187
34	25.051227345498	24.69011921114759	26.59243166710506	23.666221776249348
35	24.932211480696104	24.92376835972891	26.29610025231942	23.847919907255562
36	23.577415300883604	25.103671783414462	26.285383983399523	25.033528932302406
37	25.594513234678075	24.443643425201582	25.71141874579871	24.25042459432164
38	24.314161016288676	24.41872548255527	26.538591432208452	24.7285220689476
39	25.180715849088465	25.089464899234272	25.588909599859715	24.140909651817548
40	24.97596965682072	24.763593380614658	26.166121112929623	24.094315849635
41	23.99315455507855	25.409005591962124	26.496223314780053	24.101616538179268
42	25.129818886821315	25.073312354471323	25.808546775393026	23.988321983314336
43	24.618017555767004	24.32736991488408	26.232654506012672	24.821958023336247
44	24.023473019666934	25.329297757917434	25.975877477851967	24.671351744563662
45	24.817333359312588	25.22910456000883	25.868194246893854	24.085367833784723
46	24.14349641811014	24.696854561632538	25.74901441199202	25.410634608265298
47	24.308877349881957	24.751169866955035	26.34855378140618	24.59139900175683
48	24.652111363333756	25.546798125541926	25.12072588161557	24.680364629508748
49	24.5129653985419	25.358598405507653	25.540779710003736	24.58765648594671
50	24.074495031499644	25.534519711632136	25.675131519127103	24.71585373774112
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	69.0
2	44.0
3	86.0
4	128.0
5	139.0
6	150.0
7	102.5
8	55.0
9	90.0
10	125.0
11	213.0
12	301.0
13	605.0
14	909.0
15	1124.5
16	1340.0
17	1175.5
18	1011.0
19	824.5
20	638.0
21	695.5
22	753.0
23	923.5
24	1094.0
25	1730.0
26	2366.0
27	3055.5
28	3745.0
29	4136.5
30	4528.0
31	5511.0
32	6494.0
33	7916.5
34	9339.0
35	10383.0
36	11427.0
37	12273.5
38	13120.0
39	14759.5
40	16399.0
41	16667.5
42	16936.0
43	16121.5
44	15307.0
45	16664.5
46	18022.0
47	19080.0
48	20138.0
49	20483.0
50	20828.0
51	20918.0
52	21008.0
53	20826.0
54	20644.0
55	20735.5
56	20827.0
57	20144.0
58	19461.0
59	17823.5
60	16186.0
61	14402.5
62	12619.0
63	10749.0
64	8879.0
65	7605.5
66	6332.0
67	5955.0
68	5578.0
69	5088.0
70	4598.0
71	3648.0
72	2698.0
73	2158.5
74	1619.0
75	1259.0
76	899.0
77	697.0
78	495.0
79	467.0
80	439.0
81	296.0
82	153.0
83	132.5
84	112.0
85	91.5
86	71.0
87	45.0
88	19.0
89	12.5
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031174499259605644
2	0.005845218611176058
3	3.247343672875588E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	3.247343672875588E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.247343672875588E-4
17	0.0
18	3.247343672875588E-4
19	0.0
20	9.742031018626764E-4
21	0.0
22	0.0
23	3.247343672875588E-4
24	9.742031018626764E-4
25	6.494687345751176E-4
26	9.742031018626764E-4
27	9.742031018626764E-4
28	0.0025978749383004703
29	9.742031018626764E-4
30	0.0012989374691502352
31	9.742031018626764E-4
32	0.0012989374691502352
33	3.247343672875588E-4
34	9.742031018626764E-4
35	3.247343672875588E-4
36	3.247343672875588E-4
37	9.742031018626764E-4
38	0.0
39	6.494687345751176E-4
40	0.0
41	6.494687345751176E-4
42	0.004871015509313382
43	0.0035720780401631465
44	0.005195749876600941
45	0.0019484062037253527
46	0.0019484062037253527
47	9.742031018626764E-4
48	0.004871015509313382
49	0.002922609305588029
50	0.0012989374691502352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	307944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.296599381705764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.043496306134315	18.16564050606604
2	14.405038598821282	9.016574442106357
3	7.51328131485017	7.054204660587639
4	4.821739852245372	6.036162419141142
5	3.0702664563791817	4.804444964019432
6	2.3065908524943968	4.331306990881459
7	1.6300738773138541	3.5711038370612838
8	1.2471984726487921	3.1226456758371652
9	0.997136216485432	2.808627542670096
>10	5.617581140532913	30.89295456316733
>50	0.2604382833900556	5.461707323409451
>100	0.08612102598157217	4.525173408152131
>500	0.0010376027226695443	0.2094536669004754
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	645	0.2094536669004754	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	447	0.14515626217753877	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	410	0.1331410905878991	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	342	0.1110591536123451	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	337	0.1094354817759073	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	324	0.10521393500116906	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	324	0.10521393500116906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	3.247343672875588E-4	0.0
19	0.0	0.0	0.0	3.247343672875588E-4	0.0
20	0.0	0.0	0.0	3.247343672875588E-4	0.0
21	0.0	0.0	0.0	3.247343672875588E-4	0.0
22	0.0	0.0	0.0	6.494687345751176E-4	0.0
23	0.0	0.0	0.0	6.494687345751176E-4	0.0
24	0.0	0.0	0.0	6.494687345751176E-4	0.0
25	0.0	0.0	0.0	6.494687345751176E-4	0.0
26	0.0	0.0	0.0	9.742031018626764E-4	0.0
27	0.0	0.0	0.0	0.001623671836437794	0.0
28	0.0	0.0	0.0	0.0025978749383004703	0.0
29	0.0	0.0	0.0	0.005520484243888499	0.0
30	0.0	0.0	0.0	0.007144156080326293	0.0
31	0.0	0.0	0.0	0.017210921466240616	0.0
32	0.0	0.0	0.0	0.026952952484867378	0.0
33	0.0	0.0	0.0	0.03409710856519367	0.0
34	0.0	0.0	0.0	0.04773595199127114	0.0
35	0.0	0.0	0.0	0.062024264151923726	0.0
36	0.0	0.0	0.0	0.07923518561816434	0.0
37	0.0	0.0	0.0	0.11073441924505754	0.0
38	0.0	0.0	0.0	0.14483152781025121	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGA	35	7.2669573E-6	37.718742	40
CGCTTAC	40	1.8162424E-5	32.99854	18
ACTTAAC	35	3.2101115E-4	31.432285	38
GGTATCA	270	0.0	30.981716	1
ATTAGAA	45	4.0565435E-5	29.336798	41
ATAGCTA	45	4.0565435E-5	29.336798	40
GTTTAGG	30	0.0057396935	29.332035	25
TAGCCGA	30	0.0057396935	29.332035	9
GGGCGTT	45	4.0610423E-5	29.332035	33
GTATCAA	625	0.0	28.177057	1
TAGCGGC	55	4.947009E-6	27.998758	30
TAAACGC	40	7.021972E-4	27.498783	28
AACGCTT	40	7.021972E-4	27.498783	30
GGTCGGT	65	5.909369E-7	27.075726	11
CGACGCT	50	8.3241284E-5	26.398832	15
ACGCTTA	50	8.3241284E-5	26.398832	17
GACGCTT	50	8.3241284E-5	26.398832	16
CGCAATA	60	9.713294E-6	25.669699	36
CTAGCGG	60	9.725549E-6	25.66553	29
GGTTTGA	70	1.1281245E-6	25.141745	15
>>END_MODULE