FastQCFastQC Report
Thu 2 Feb 2017
SRR4062062_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062062_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034691
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34320.1686742606125451No Hit
GCGCAAGACGGACCAGAGCGAAAGC20860.10252170968466465No Hit
TATCAACGCAGAGTACTTTTTTTTT20450.10050666169949148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA456.767383E-414.7766863
GGTATCA8000.012.9370631
TACCTCG604.0895896E-412.6679147
GTATCAA22150.012.5172321
CGGCGTC1850.012.32493114
AACCGCG853.940655E-612.2953287
CGCGTAA1501.4551915E-1112.03481410
AGAACCG1201.000808E-811.8758775
TCGGCGT2100.011.76248413
CGTATAT2200.011.66052510
AGCGTAT2050.011.5867918
CGGACAT2000.011.4008435
TCGCGTA1604.5474735E-1111.2829179
GCGTAAC1604.5474735E-1111.28291711
GTATTAT1701.2732926E-1111.1706961
CGGTCGG2050.011.1233210
GTGTACG600.005899362311.0776071
ACCGTCG3350.011.0610638
GTACTAA951.3684699E-510.9943171
GCGGTCG2100.010.8587479
Produced by FastQC (version 0.11.5)