##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062062_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2034691
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3002608258453	32.0	32.0	32.0	32.0	32.0
2	30.88613160425834	32.0	32.0	32.0	32.0	32.0
3	30.906988825330235	32.0	32.0	32.0	32.0	32.0
4	30.90620443104137	32.0	32.0	32.0	32.0	32.0
5	30.84764713659224	32.0	32.0	32.0	32.0	32.0
6	34.50182755022753	36.0	36.0	36.0	32.0	36.0
7	34.439667743161	36.0	36.0	36.0	32.0	36.0
8	34.40761865069438	36.0	36.0	36.0	32.0	36.0
9	34.52359105141763	36.0	36.0	36.0	32.0	36.0
10	34.24652047903097	36.0	36.0	36.0	32.0	36.0
11	34.50193420032821	36.0	36.0	36.0	32.0	36.0
12	34.33926183386077	36.0	36.0	36.0	32.0	36.0
13	34.42214468929189	36.0	36.0	36.0	32.0	36.0
14	34.33239543498251	36.0	36.0	36.0	32.0	36.0
15	34.28733748760868	36.0	36.0	36.0	32.0	36.0
16	34.28706914219407	36.0	36.0	36.0	32.0	36.0
17	34.231045893455075	36.0	36.0	36.0	32.0	36.0
18	34.236468338435664	36.0	36.0	36.0	32.0	36.0
19	34.23353177460361	36.0	36.0	36.0	32.0	36.0
20	34.220723441544685	36.0	36.0	36.0	32.0	36.0
21	34.206552739457734	36.0	36.0	36.0	32.0	36.0
22	34.1767280633767	36.0	36.0	36.0	32.0	36.0
23	34.15020167681481	36.0	36.0	36.0	32.0	36.0
24	34.12663347899018	36.0	36.0	36.0	32.0	36.0
25	33.78655579643297	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	34.0
5	159.0
6	438.0
7	77.0
8	356.0
9	295.0
10	174.0
11	37.0
12	82.0
13	86.0
14	287.0
15	415.0
16	690.0
17	931.0
18	1241.0
19	1536.0
20	2387.0
21	3245.0
22	5100.0
23	7235.0
24	10621.0
25	15227.0
26	21263.0
27	27651.0
28	37253.0
29	50186.0
30	66391.0
31	90835.0
32	129451.0
33	186490.0
34	416356.0
35	958161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.450783415573945	16.84957831206873	11.664635225195116	26.03500304716221
2	16.899470238969947	19.167529615061945	37.03756491771969	26.895435228248417
3	19.46565448258101	22.504172654516	27.895104675142377	30.135068187760606
4	13.101925216960469	15.165212399089297	34.78219410245017	36.950668281500064
5	15.379112702086015	35.75937340367842	33.0975386595594	15.763975234676172
6	35.444509635070744	34.2959141870595	16.314284829048376	13.945291348821382
7	31.15782772187889	29.7916054747289	19.92232375368935	19.12824304970286
8	28.47450672056634	32.77982186735209	18.962585452402017	19.783085959679553
9	27.136966416533397	14.62910567105425	18.398179357854595	39.83574855455775
10	16.485905768108324	26.438483714786564	30.848104741910053	26.22750577519506
11	37.401861964965605	21.17222290031853	21.753816260399482	19.67209887431638
12	24.78033632386305	23.309940599516725	28.32507369695419	23.58464937966604
13	29.430589045814674	19.420107278185128	24.930269179666357	26.219034496333844
14	23.64465701253266	19.39969197309439	24.73219865275133	32.22345236162162
15	25.265311907588185	27.01402791803727	21.78057706201143	25.940083112363112
16	26.080039759083572	25.70744597094831	23.29532929181593	24.91718497815219
17	24.307315135997765	25.859283539578442	24.882426263293326	24.95097506113047
18	24.991278719337135	25.080139835710263	25.55632914039417	24.372252304558437
19	25.64788162130171	24.921593003857502	25.05447287622198	24.376052498618808
20	25.869079911175913	24.44315229080147	24.56958001399068	25.11818778403193
21	26.522937093141035	24.21454359235714	24.468675948778014	24.79384336572381
22	26.017300128998233	24.188054035598004	24.761461507655767	25.033184327748003
23	24.735711812250617	24.342304604802344	25.059960413338267	25.86202316960878
24	24.836248273935055	24.785579320770385	25.128012896478406	25.25015950881615
25	25.084413425079642	24.378115081668515	25.04751944558679	25.489952047665053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	502.0
1	502.0
2	606.5
3	711.0
4	711.0
5	711.0
6	1416.5
7	2122.0
8	2122.0
9	2122.0
10	2641.5
11	3161.0
12	3161.0
13	3161.0
14	4174.5
15	5188.0
16	5188.0
17	5188.0
18	8587.0
19	11986.0
20	11986.0
21	11986.0
22	18742.0
23	25498.0
24	25498.0
25	25498.0
26	37302.5
27	49107.0
28	49107.0
29	49107.0
30	62668.5
31	76230.0
32	76230.0
33	76230.0
34	95919.0
35	115608.0
36	115608.0
37	115608.0
38	137456.0
39	159304.0
40	159304.0
41	159304.0
42	184971.0
43	210638.0
44	210638.0
45	210638.0
46	235728.0
47	260818.0
48	260818.0
49	260818.0
50	271809.0
51	282800.0
52	282800.0
53	282800.0
54	267512.0
55	252224.0
56	252224.0
57	252224.0
58	232740.0
59	213256.0
60	213256.0
61	213256.0
62	187096.5
63	160937.0
64	160937.0
65	160937.0
66	130832.0
67	100727.0
68	100727.0
69	100727.0
70	76356.5
71	51986.0
72	51986.0
73	51986.0
74	39223.0
75	26460.0
76	26460.0
77	26460.0
78	20797.0
79	15134.0
80	15134.0
81	15134.0
82	10435.0
83	5736.0
84	5736.0
85	5736.0
86	4182.0
87	2628.0
88	2628.0
89	2628.0
90	1765.5
91	903.0
92	903.0
93	903.0
94	549.0
95	195.0
96	195.0
97	195.0
98	513.5
99	832.0
100	832.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.406226301684138E-4
2	0.0
3	7.372126775023824E-4
4	0.010763305091534782
5	0.03528791349644737
6	0.06575937083321251
7	0.10576544546567514
8	0.11171229439752768
9	0.11996907638555437
10	0.13392696974626614
11	0.14051273633195407
12	0.13373037969893217
13	0.12734120316057818
14	0.11800317591221468
15	0.134811624959269
16	0.12046055150388929
17	0.12591592531740692
18	0.11333416228803293
19	0.0996711539983221
20	0.09382260009013654
21	0.09298709238896717
22	0.103455512409501
23	0.08271526241576731
24	0.0927905023416332
25	0.09038227426179209
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2034691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.89911474613012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.12473064370849	38.98907015949944
2	14.179352443277763	14.717916789589857
3	4.534372867783707	7.0598981330053725
4	2.0498490380841075	4.255414017590861
5	1.0380462314983818	2.693684024016123
6	0.6779100102602433	2.1109757646027965
7	0.4435058810208393	1.6112293826778847
8	0.3063769397852316	1.2720553562785544
9	0.22742582871113728	1.0622879262461742
>10	1.2638571676372972	11.791824668089063
>50	0.08172711434800503	2.9451789733458478
>100	0.06235942382695727	6.706388269237341
>500	0.007817142043206535	2.7663980084170046
>1k	0.0026692680147534487	2.0176785274037594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3432	0.1686742606125451	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2086	0.10252170968466465	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2045	0.10050666169949148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.9147511833492163E-5	0.0
10	0.0	0.0	0.0	4.9147511833492163E-5	4.9147511833492163E-5
11	0.0	0.0	0.0	4.9147511833492163E-5	4.9147511833492163E-5
12	0.0	0.0	0.0	4.9147511833492163E-5	2.94885071000953E-4
13	0.0	0.0	0.0	4.9147511833492163E-5	3.440325828344451E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACTA	45	6.767383E-4	14.776686	3
GGTATCA	800	0.0	12.937063	1
TACCTCG	60	4.0895896E-4	12.667914	7
GTATCAA	2215	0.0	12.517232	1
CGGCGTC	185	0.0	12.324931	14
AACCGCG	85	3.940655E-6	12.295328	7
CGCGTAA	150	1.4551915E-11	12.034814	10
AGAACCG	120	1.000808E-8	11.875877	5
TCGGCGT	210	0.0	11.762484	13
CGTATAT	220	0.0	11.660525	10
AGCGTAT	205	0.0	11.586791	8
CGGACAT	200	0.0	11.400843	5
TCGCGTA	160	4.5474735E-11	11.282917	9
GCGTAAC	160	4.5474735E-11	11.282917	11
GTATTAT	170	1.2732926E-11	11.170696	1
CGGTCGG	205	0.0	11.12332	10
GTGTACG	60	0.0058993623	11.077607	1
ACCGTCG	335	0.0	11.061063	8
GTACTAA	95	1.3684699E-5	10.994317	1
GCGGTCG	210	0.0	10.858747	9
>>END_MODULE