FastQCFastQC Report
Thu 2 Feb 2017
SRR4062062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034691
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20910.1027674472438321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25150.028.1714061
GGTATCA10850.021.2936521
TCAACGC37000.018.9668064
ATCAACG37100.018.915683
CAACGCA38100.018.4769485
AACGCAG38500.018.2278396
ACCGTCG5500.017.9992758
ATACCGT5850.017.6745016
TACCGTC5900.017.524727
TATCAAC40400.017.4794962
CGTCGTA5850.017.29844910
CCGTCGT5900.016.7789869
GTACCGT1854.2382453E-1016.647986
GTAGGAC5400.015.8882493
TAGGACG3200.015.8118654
TACACCG1958.931238E-1015.7942375
GCGAAAG6900.015.62294318
CGAGTAT850.002981676315.52878715
GATATAC2001.2751116E-915.4024091
AGCGAAA6900.015.30373217
Produced by FastQC (version 0.11.5)