##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2034691
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31396757542054	32.0	32.0	32.0	32.0	32.0
2	31.484582671275394	32.0	32.0	32.0	32.0	32.0
3	31.59040660228015	32.0	32.0	32.0	32.0	32.0
4	31.661218828804962	32.0	32.0	32.0	32.0	32.0
5	31.640625529871613	32.0	32.0	32.0	32.0	32.0
6	35.287269664042356	36.0	36.0	36.0	36.0	36.0
7	35.29336149813412	36.0	36.0	36.0	36.0	36.0
8	35.24977797611529	36.0	36.0	36.0	36.0	36.0
9	35.323957790150935	36.0	36.0	36.0	36.0	36.0
10	35.20753716411976	36.0	36.0	36.0	36.0	36.0
11	35.32153678371802	36.0	36.0	36.0	36.0	36.0
12	35.24617644644813	36.0	36.0	36.0	36.0	36.0
13	35.27800879838757	36.0	36.0	36.0	36.0	36.0
14	35.24689596602138	36.0	36.0	36.0	36.0	36.0
15	35.22481349747947	36.0	36.0	36.0	36.0	36.0
16	35.234145627026415	36.0	36.0	36.0	36.0	36.0
17	35.21879685908081	36.0	36.0	36.0	36.0	36.0
18	35.21459376386881	36.0	36.0	36.0	36.0	36.0
19	35.20552260760971	36.0	36.0	36.0	36.0	36.0
20	35.206258345861855	36.0	36.0	36.0	36.0	36.0
21	35.196236185248765	36.0	36.0	36.0	36.0	36.0
22	35.1770873316882	36.0	36.0	36.0	36.0	36.0
23	35.1513836744744	36.0	36.0	36.0	36.0	36.0
24	35.13716726520145	36.0	36.0	36.0	36.0	36.0
25	35.11692979425377	36.0	36.0	36.0	36.0	36.0
26	35.07972168746999	36.0	36.0	36.0	36.0	36.0
27	35.06623757612335	36.0	36.0	36.0	36.0	36.0
28	35.049616870571505	36.0	36.0	36.0	36.0	36.0
29	35.026282123428075	36.0	36.0	36.0	36.0	36.0
30	35.011543767579454	36.0	36.0	36.0	36.0	36.0
31	35.01398345006687	36.0	36.0	36.0	36.0	36.0
32	34.99022357694608	36.0	36.0	36.0	36.0	36.0
33	34.97252064318366	36.0	36.0	36.0	36.0	36.0
34	34.95768399231137	36.0	36.0	36.0	36.0	36.0
35	34.94230721028402	36.0	36.0	36.0	36.0	36.0
36	34.924305951124765	36.0	36.0	36.0	36.0	36.0
37	34.909006330691	36.0	36.0	36.0	36.0	36.0
38	34.89098737842749	36.0	36.0	36.0	32.0	36.0
39	34.880536651511214	36.0	36.0	36.0	32.0	36.0
40	34.868814478463804	36.0	36.0	36.0	32.0	36.0
41	34.85186497605779	36.0	36.0	36.0	32.0	36.0
42	34.81330187237276	36.0	36.0	36.0	32.0	36.0
43	34.81292294505652	36.0	36.0	36.0	32.0	36.0
44	34.77138395952997	36.0	36.0	36.0	32.0	36.0
45	34.75239385243263	36.0	36.0	36.0	32.0	36.0
46	34.741990798602835	36.0	36.0	36.0	32.0	36.0
47	34.70828396056207	36.0	36.0	36.0	32.0	36.0
48	34.68941082454289	36.0	36.0	36.0	32.0	36.0
49	34.67243035920442	36.0	36.0	36.0	32.0	36.0
50	34.269550020125905	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	6.0
20	13.0
21	61.0
22	138.0
23	363.0
24	964.0
25	2208.0
26	4553.0
27	8842.0
28	15069.0
29	24410.0
30	36381.0
31	53735.0
32	81563.0
33	139124.0
34	320465.0
35	1346796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.983650545653695	17.469177620873182	11.972819081311693	26.574352752161424
2	16.28486484121995	19.59842859087775	36.74552382843468	27.37118273946762
3	18.698794209611133	23.024236711191232	28.16410623379968	30.112862845397952
4	12.795112378243184	15.523683940215	34.91070634312532	36.7704973384165
5	15.019283026267871	36.098798294188164	33.00442180163966	15.87749687790431
6	35.62118614745848	34.41770925075491	16.16468962317564	13.796414978610972
7	31.15382139106135	29.777740207235396	19.97050166339754	19.09793673830572
8	28.481474582627044	32.905684450366174	18.835980500233205	19.77686046677358
9	27.230916144023837	14.37667930904496	18.29167180667728	40.100732740253925
10	16.449139672382525	26.492979274484075	30.796583263298093	26.261297789835307
11	37.57263387905092	21.10502282656187	21.704327585859478	19.618015708527732
12	24.85478138940999	23.288155302205592	28.258590616462154	23.59847269192226
13	29.566455053863216	19.297180751278695	24.97735528392272	26.159008910935373
14	23.676322350666513	19.147723167793046	24.82313039178922	32.35282408975122
15	25.327334715689016	26.971368134031167	21.828474200750875	25.872822949528945
16	26.216309198498045	25.62742052804372	23.231122338647847	24.925147934810386
17	24.38458714369897	25.807260168743067	24.81005715364151	24.998095533916455
18	25.1357947754152	24.88902475465526	25.632529409914444	24.342651060015097
19	25.76307655560476	24.823916751978555	25.062675364465658	24.350331327951025
20	25.959140624155275	24.2523907865882	24.726567145987143	25.06190144326938
21	26.56752302929536	24.15801711414657	24.465336505641396	24.809123350916675
22	26.083518332759127	24.072254705997125	24.848343065359803	24.995883895883946
23	24.706650788743843	24.142486500407188	25.278924416533027	25.871938294315942
24	24.995564380377115	24.57038382239828	25.153231613066524	25.28082018415808
25	25.214013786921	24.21095778111764	25.143044465916542	25.431983966044818
26	24.81649475618561	25.11143013432559	25.338492531760476	24.733582577728324
27	25.401229182895634	24.6319700592475	24.849251849794932	25.117548908061938
28	24.994605975743355	24.51550849552677	25.2219659412216	25.267919587508274
29	24.90226993255962	24.700174867276818	25.231903104459363	25.1656520957042
30	24.853919441283946	24.793762118088235	25.44787471156185	24.904443729065964
31	25.412349042898065	24.528821183043657	24.62279154895797	25.43603822510031
32	25.306300784937996	24.59591985582029	24.566873562122453	25.530905797119257
33	24.882254617049206	24.375985102376927	25.080614364764703	25.661145915809165
34	25.45190308221518	24.468944149747895	25.176920320857455	24.902232447179472
35	25.7827989183589	24.302423076582823	25.218633243655287	24.696144761402987
36	24.891604896283752	24.84722458403901	24.90389180443567	25.357278715241566
37	25.730747548856986	24.588985989325995	24.731564883226362	24.948701578590658
38	25.029021620001078	24.489430822385717	25.065538239240375	25.416009318372822
39	25.692627001480822	24.399156427960943	24.659492077627892	25.248724492930343
40	25.86545082275392	24.448577204106176	24.921622005503536	24.764349967636363
41	24.868983474937174	24.89041204285517	25.26270883638453	24.977895645823125
42	25.86248250263444	24.848519998112646	24.90779478145987	24.38120271779305
43	25.430035810513917	24.182811543115644	25.033544710060664	25.35360793630978
44	24.953467580653328	24.728018193229833	24.859395174390286	25.459119051726553
45	25.210736593879336	24.858292511386484	24.83661801126386	25.094352883470318
46	25.030607304959833	24.66848025085457	24.87564169670876	25.42527074747683
47	25.220392762830997	24.608792709161172	25.166329755836713	25.004484772171114
48	25.785069861535163	25.030423730313046	24.28182302530822	24.90268338284357
49	25.123783179642643	25.05045202855	24.469160122402076	25.35660466940528
50	25.286572206661283	25.20567389132201	24.46766107046263	25.040092831554077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	258.0
1	157.5
2	57.0
3	202.5
4	348.0
5	354.0
6	360.0
7	303.5
8	247.0
9	337.5
10	428.0
11	665.0
12	902.0
13	1746.5
14	2591.0
15	3255.5
16	3920.0
17	3776.0
18	3632.0
19	3556.5
20	3481.0
21	4118.0
22	4755.0
23	5843.5
24	6932.0
25	8749.5
26	10567.0
27	13397.0
28	16227.0
29	18997.0
30	21767.0
31	25542.5
32	29318.0
33	33773.5
34	38229.0
35	44368.5
36	50508.0
37	59140.0
38	67772.0
39	75331.5
40	82891.0
41	90928.0
42	98965.0
43	104176.0
44	109387.0
45	120302.5
46	131218.0
47	140114.0
48	149010.0
49	155830.0
50	162650.0
51	160321.5
52	157993.0
53	157370.5
54	156748.0
55	156387.5
56	156027.0
57	150113.5
58	144200.0
59	132581.5
60	120963.0
61	106435.5
62	91908.0
63	79309.5
64	66711.0
65	56505.5
66	46300.0
67	39734.0
68	33168.0
69	29210.0
70	25252.0
71	19764.0
72	14276.0
73	12300.5
74	10325.0
75	7665.5
76	5006.0
77	4368.0
78	3730.0
79	3113.0
80	2496.0
81	1976.5
82	1457.0
83	1179.5
84	902.0
85	700.0
86	498.0
87	332.5
88	167.0
89	113.0
90	59.0
91	44.0
92	29.0
93	22.5
94	16.0
95	15.0
96	14.0
97	13.0
98	12.0
99	13.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030324014801264666
2	0.004128390994013342
3	4.423276065014295E-4
4	0.0
5	0.0
6	1.4744253550047647E-4
7	0.0
8	0.0
9	0.0
10	4.9147511833492163E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.406226301684138E-4
17	0.0
18	1.4744253550047647E-4
19	0.0
20	2.457375591674608E-4
21	0.0
22	0.0
23	0.0
24	0.0012778353076707961
25	4.423276065014295E-4
26	3.931800946679373E-4
27	2.9488507100095294E-4
28	0.0015727203786717492
29	2.457375591674608E-4
30	0.0012778353076707961
31	3.440325828344451E-4
32	3.931800946679373E-4
33	1.9659004733396865E-4
34	8.84655213002859E-4
35	2.457375591674608E-4
36	2.457375591674608E-4
37	0.0013761303313377805
38	4.9147511833492163E-5
39	4.914751183349216E-4
40	0.0
41	0.0011795402840038118
42	0.004865603671515724
43	0.00457071860051477
44	0.0043249810413473104
45	0.002064195497006671
46	0.0022607855443406394
47	0.0013761303313377805
48	0.004669013624181755
49	0.004177538505846834
50	0.0018676054496727021
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2034691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.333112703503474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8331468953088	39.91084657325335
2	14.58844524051392	15.560943883624354
3	4.614085576853628	7.382506382818331
4	2.0483892468296676	4.36987898247245
5	1.006964282244538	2.6852269776675213
6	0.6303491394913393	2.017108901942882
7	0.41665797801513726	1.555516683020663
8	0.30476067402511825	1.300306830030185
9	0.21831715042025407	1.0479179869624016
>10	1.2001594547118595	11.409878949979541
>50	0.07564094022528178	2.774557520111226
>100	0.05425933993790014	5.951241837428447
>500	0.006687724867750411	2.432538395573972
>1k	0.0021363565549758225	1.6015300951146716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2091	0.1027674472438321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.9147511833492163E-5
9	0.0	0.0	0.0	0.0	4.9147511833492163E-5
10	0.0	0.0	0.0	0.0	4.9147511833492163E-5
11	0.0	0.0	0.0	0.0	4.9147511833492163E-5
12	0.0	0.0	0.0	0.0	1.474425355004765E-4
13	0.0	0.0	0.0	0.0	2.457375591674608E-4
14	0.0	0.0	0.0	0.0	2.457375591674608E-4
15	0.0	0.0	0.0	4.9147511833492163E-5	2.94885071000953E-4
16	0.0	0.0	0.0	4.9147511833492163E-5	2.94885071000953E-4
17	0.0	0.0	0.0	4.9147511833492163E-5	2.94885071000953E-4
18	0.0	0.0	0.0	4.9147511833492163E-5	3.931800946679373E-4
19	0.0	0.0	0.0	9.829502366698433E-5	3.931800946679373E-4
20	0.0	0.0	0.0	1.9659004733396865E-4	3.931800946679373E-4
21	0.0	0.0	0.0	2.94885071000953E-4	3.931800946679373E-4
22	0.0	0.0	0.0	3.931800946679373E-4	3.931800946679373E-4
23	0.0	0.0	0.0	5.89770142001906E-4	3.931800946679373E-4
24	0.0	0.0	0.0	0.0010320977485033353	3.931800946679373E-4
25	0.0	0.0	0.0	0.001228687795837304	3.931800946679373E-4
26	0.0	0.0	0.0	0.001523572866838257	4.4232760650142944E-4
27	0.0	0.0	0.0	0.0019167529615061944	4.4232760650142944E-4
28	0.0	0.0	0.0	0.002752260662675561	4.4232760650142944E-4
29	0.0	0.0	0.0	0.00363691587567842	4.4232760650142944E-4
30	0.0	0.0	0.0	0.007322979263190332	4.4232760650142944E-4
31	0.0	0.0	0.0	0.013613860777877329	4.4232760650142944E-4
32	4.9147511833492163E-5	0.0	0.0	0.019953889804397818	5.406226301684138E-4
33	4.9147511833492163E-5	0.0	0.0	0.02663795141375275	5.406226301684138E-4
34	4.9147511833492163E-5	0.0	0.0	0.033960930676943085	5.89770142001906E-4
35	4.9147511833492163E-5	0.0	0.0	0.042660040271471196	5.89770142001906E-4
36	4.9147511833492163E-5	0.0	0.0	0.05396396799317439	5.89770142001906E-4
37	4.9147511833492163E-5	0.0	0.0	0.07308235009640285	5.89770142001906E-4
38	4.9147511833492163E-5	0.0	0.0	0.09667315577647909	5.89770142001906E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2515	0.0	28.171406	1
GGTATCA	1085	0.0	21.293652	1
TCAACGC	3700	0.0	18.966806	4
ATCAACG	3710	0.0	18.91568	3
CAACGCA	3810	0.0	18.476948	5
AACGCAG	3850	0.0	18.227839	6
ACCGTCG	550	0.0	17.999275	8
ATACCGT	585	0.0	17.674501	6
TACCGTC	590	0.0	17.52472	7
TATCAAC	4040	0.0	17.479496	2
CGTCGTA	585	0.0	17.298449	10
CCGTCGT	590	0.0	16.778986	9
GTACCGT	185	4.2382453E-10	16.64798	6
GTAGGAC	540	0.0	15.888249	3
TAGGACG	320	0.0	15.811865	4
TACACCG	195	8.931238E-10	15.794237	5
GCGAAAG	690	0.0	15.622943	18
CGAGTAT	85	0.0029816763	15.528787	15
GATATAC	200	1.2751116E-9	15.402409	1
AGCGAAA	690	0.0	15.303732	17
>>END_MODULE