FastQCFastQC Report
Thu 2 Feb 2017
SRR4062061_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062061_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476599
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGTATAAGGGAGCTTGAC42010.28450513646562137No Hit
GGCCTATTCACTGCGGCTGACGTAA36050.24414211305845393No Hit
GTATCAACGCAGAGTACTTTTTTTT33030.22368970858032544No Hit
GTCCTAAGGTAGCGAAATTCCTTGT30030.20337275048946937No Hit
CCTTTACACTCTGCGAATGATTTCC29630.20066382274402192No Hit
CCCTTATACCTTTACACTCTGCGAA25030.16951115367137592No Hit
CGTTGAGACAGTGCCCAAATCATTA24810.1680212434113798No Hit
TTCCAGCACCGGGCAGGCGTCACCC23100.15644057729959185No Hit
GTGCTGGAAGGTTAAGAGGAGTGCT22960.15549245258868521No Hit
GTATAAGGGAGCTTGACTGCGAGAG22610.15312214081141867No Hit
GAATAGGCCCAAGCAACTGTTTATC22410.15176767693869492No Hit
TCCCTACATAGCTACCCAGCGATGC21010.14228642982962877No Hit
GTGATGGGGAGCGAAGTTTAGTAGC20900.14154147469963071No Hit
GTCTCACGACGTTCTGAACCCAGCT20760.14059334998872408No Hit
GTCTCAACGAGAGACTCGGTGAAAT20290.1374103598878233No Hit
GTACTAGAGTATGTAGTGATGGAGG20100.13612361920873575No Hit
GTTCTATAACATTGAGCTGTGATGG19440.13165388842874742No Hit
GTATAGGGGGTGACGCCTGCCCGGT19420.13151844204147503No Hit
CTCTAACCCAGATAGGTGATCCCTA19130.1295544694260256No Hit
TATCAACGCAGAGTACTTTTTTTTT19000.12867406790875519No Hit
GGTGTGAACTGAGTCAAATGCTTAG18670.12643920251876103No Hit
GTTAAGAGGAGTGCTTAGCGTAAGC18470.12508473864603728No Hit
CCTCTTAACCTTCCAGCACCGGGCA18160.12298531964331548No Hit
CCCCCTATACATCATCTTACGATTT17920.121359962996047No Hit
GCCCAACCCTTGGGACCGACTACAG17220.11661933944151391No Hit
GATAGGGACCGAACTGTCTCACGAC16470.1115400999187999No Hit
GGATAGGTAGGAGTCTATGAGATCG16200.10971157369062284No Hit
GGTATAAGGGAGCTTGACTGCGAGA16180.10957612730335047No Hit
CTATAACATTGAGCTGTGATGGGGA15980.10822166343062672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATC1001.8189894E-1216.15184612
GGTATCA5850.016.0694921
TAGGCCC4050.015.246074
CGAATCG1101.2732926E-1114.68299813
ATAGGCC4300.014.1377933
GACGCTG551.958145E-413.81976111
CCTTATA4250.013.6326892
AATAGGC4400.013.5996752
CCCTTAT4300.013.4705141
AGTTCGG1205.0931703E-1113.46032810
TTATACC4450.013.4486944
CTGACGT6400.013.35818317
CGTTACG1500.013.30061716
CCACGCG500.001500492213.29881318
GCGAAAT3600.013.19595212
CGCATAG1950.013.15579410
CGTAGGA1102.752131E-912.95251119
ATCGAAT1251.0186341E-1012.92147611
AATTCCT4050.012.90183416
GTATCAA18550.012.6949391
Produced by FastQC (version 0.11.5)