##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062061_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1476599
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31949026106614	32.0	32.0	32.0	32.0	32.0
2	30.94570970182155	32.0	32.0	32.0	32.0	32.0
3	30.97688201062035	32.0	32.0	32.0	32.0	32.0
4	30.966183777721643	32.0	32.0	32.0	32.0	32.0
5	30.940113057099456	32.0	32.0	32.0	32.0	32.0
6	34.58462182352826	36.0	36.0	36.0	32.0	36.0
7	34.54041009102674	36.0	36.0	36.0	32.0	36.0
8	34.50578728551218	36.0	36.0	36.0	32.0	36.0
9	34.58379763226171	36.0	36.0	36.0	32.0	36.0
10	34.3618050669139	36.0	36.0	36.0	32.0	36.0
11	34.58761044806342	36.0	36.0	36.0	32.0	36.0
12	34.46111774422169	36.0	36.0	36.0	32.0	36.0
13	34.5091158804794	36.0	36.0	36.0	32.0	36.0
14	34.4404723286417	36.0	36.0	36.0	32.0	36.0
15	34.400981579968565	36.0	36.0	36.0	32.0	36.0
16	34.413149406169175	36.0	36.0	36.0	32.0	36.0
17	34.37679762752108	36.0	36.0	36.0	32.0	36.0
18	34.36802882840907	36.0	36.0	36.0	32.0	36.0
19	34.37557928726756	36.0	36.0	36.0	32.0	36.0
20	34.35455665349902	36.0	36.0	36.0	32.0	36.0
21	34.34210709881288	36.0	36.0	36.0	32.0	36.0
22	34.32376698074427	36.0	36.0	36.0	32.0	36.0
23	34.29314119811811	36.0	36.0	36.0	32.0	36.0
24	34.25901412638096	36.0	36.0	36.0	32.0	36.0
25	33.96132870196986	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	18.0
5	118.0
6	307.0
7	52.0
8	280.0
9	208.0
10	103.0
11	30.0
12	71.0
13	64.0
14	246.0
15	322.0
16	567.0
17	647.0
18	909.0
19	1133.0
20	1652.0
21	2348.0
22	3468.0
23	5213.0
24	7347.0
25	10328.0
26	14278.0
27	18637.0
28	24960.0
29	33362.0
30	43751.0
31	59892.0
32	85529.0
33	124726.0
34	291856.0
35	744177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.135562090184195	18.343629662638886	11.861615166960364	25.659193080216554
2	16.574359641174215	18.88027446948857	39.29279138341345	25.252574505923768
3	19.020826335236816	22.8695536749488	29.664592521156823	28.445027468657557
4	12.615750774832572	15.903506252844664	37.859240555712084	33.621502416610674
5	13.672250781116624	36.877376206903094	34.89538592445457	14.554987087525708
6	32.013295700582454	38.28877150301728	16.444586000210073	13.253346796190193
7	29.21247302467391	31.659727490730248	19.883467947244636	19.244331537351208
8	27.13331082354632	35.111674307790146	18.552268132243643	19.202746736419886
9	27.456654845774654	15.26658032669058	18.98432997240285	38.292434855131916
10	16.391319179064144	27.58387072491406	31.48903300711195	24.535777088909853
11	35.99356509307149	22.776708458294507	22.687184036439152	18.542542412194855
12	22.6593181385085	25.363476133220214	29.850445652019115	22.126760076252168
13	28.887607990994535	20.749935578370607	25.89281597113911	24.469640459495746
14	22.26726659533699	21.00848768885074	25.566874282126495	31.157371433685775
15	24.98748777599796	28.203027619123834	23.075321553984313	23.734163050893894
16	24.98777787948752	28.066057086287156	23.379543458877055	23.56662157534827
17	22.614875732679472	27.639883801986	25.420485460330205	24.324755005004324
18	22.813482487858426	27.657365866358933	26.836559447443914	22.692592198338723
19	25.851228329920083	26.19282464699793	25.605013242873692	22.350933780208294
20	23.59658791263169	26.44957450587232	26.570844060503273	23.382993520992716
21	25.878453263378375	24.26250732934075	25.67984083891719	24.179198568363685
22	24.13152567560055	25.6720752675488	26.180123086685402	24.01627597016524
23	23.734750549180177	26.435815562217492	26.95710463941179	22.87232924919055
24	24.136500531775358	27.078448558105862	25.15368612849238	23.6313647816264
25	23.29140677214735	26.60278266197964	25.767550251545813	24.338260314327197
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	105.0
1	105.0
2	292.0
3	479.0
4	479.0
5	479.0
6	1038.0
7	1597.0
8	1597.0
9	1597.0
10	1984.5
11	2372.0
12	2372.0
13	2372.0
14	3323.5
15	4275.0
16	4275.0
17	4275.0
18	7004.5
19	9734.0
20	9734.0
21	9734.0
22	15200.5
23	20667.0
24	20667.0
25	20667.0
26	31152.0
27	41637.0
28	41637.0
29	41637.0
30	56245.0
31	70853.0
32	70853.0
33	70853.0
34	88510.0
35	106167.0
36	106167.0
37	106167.0
38	124948.0
39	143729.0
40	143729.0
41	143729.0
42	171656.5
43	199584.0
44	199584.0
45	199584.0
46	204477.0
47	209370.0
48	209370.0
49	209370.0
50	207903.5
51	206437.0
52	206437.0
53	206437.0
54	187571.5
55	168706.0
56	168706.0
57	168706.0
58	146824.0
59	124942.0
60	124942.0
61	124942.0
62	105198.0
63	85454.0
64	85454.0
65	85454.0
66	65935.0
67	46416.0
68	46416.0
69	46416.0
70	33895.0
71	21374.0
72	21374.0
73	21374.0
74	14266.0
75	7158.0
76	7158.0
77	7158.0
78	5173.5
79	3189.0
80	3189.0
81	3189.0
82	2169.0
83	1149.0
84	1149.0
85	1149.0
86	791.0
87	433.0
88	433.0
89	433.0
90	290.0
91	147.0
92	147.0
93	147.0
94	114.5
95	82.0
96	82.0
97	82.0
98	312.5
99	543.0
100	543.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001422187066359926
2	3.3861596818093474E-4
3	4.740623554533086E-4
4	0.010226202239064229
5	0.03433565917354678
6	0.0632534628561986
7	0.11106603756334658
8	0.11411358127697499
9	0.12251125728786216
10	0.138019868630549
11	0.14499535757507623
12	0.13666540475782524
13	0.13131527246056646
14	0.11898965121878045
15	0.13822303821145754
16	0.12284987325604312
17	0.12698098806785052
18	0.1156712147306073
19	0.09928220187065005
20	0.09298394486248467
21	0.09237443611975898
22	0.10436144139336409
23	0.08248684984887569
24	0.09156175779612474
25	0.08946233879340296
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1476599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.456899879974852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.771158717536146	18.81357996530034
2	15.542025734022106	9.467238434262077
3	7.660244287451308	6.999218799571614
4	4.1965730584453285	5.112584219202769
5	2.64427012420549	4.026813521426764
6	1.8225726464093772	3.3305947569402727
7	1.270356454882891	2.7083783550773606
8	0.8846060932071372	2.1553887371220384
9	0.6593262914284896	1.807293136164537
>10	3.109656379478174	16.420109527834676
>50	0.21105623734371873	4.483886987519306
>100	0.18890168407996746	12.312460407145656
>500	0.02497873096085635	5.134685868518786
>1k	0.014273560549060775	7.227767283913773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAAGGTATAAGGGAGCTTGAC	4201	0.28450513646562137	No Hit
GGCCTATTCACTGCGGCTGACGTAA	3605	0.24414211305845393	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3303	0.22368970858032544	No Hit
GTCCTAAGGTAGCGAAATTCCTTGT	3003	0.20337275048946937	No Hit
CCTTTACACTCTGCGAATGATTTCC	2963	0.20066382274402192	No Hit
CCCTTATACCTTTACACTCTGCGAA	2503	0.16951115367137592	No Hit
CGTTGAGACAGTGCCCAAATCATTA	2481	0.1680212434113798	No Hit
TTCCAGCACCGGGCAGGCGTCACCC	2310	0.15644057729959185	No Hit
GTGCTGGAAGGTTAAGAGGAGTGCT	2296	0.15549245258868521	No Hit
GTATAAGGGAGCTTGACTGCGAGAG	2261	0.15312214081141867	No Hit
GAATAGGCCCAAGCAACTGTTTATC	2241	0.15176767693869492	No Hit
TCCCTACATAGCTACCCAGCGATGC	2101	0.14228642982962877	No Hit
GTGATGGGGAGCGAAGTTTAGTAGC	2090	0.14154147469963071	No Hit
GTCTCACGACGTTCTGAACCCAGCT	2076	0.14059334998872408	No Hit
GTCTCAACGAGAGACTCGGTGAAAT	2029	0.1374103598878233	No Hit
GTACTAGAGTATGTAGTGATGGAGG	2010	0.13612361920873575	No Hit
GTTCTATAACATTGAGCTGTGATGG	1944	0.13165388842874742	No Hit
GTATAGGGGGTGACGCCTGCCCGGT	1942	0.13151844204147503	No Hit
CTCTAACCCAGATAGGTGATCCCTA	1913	0.1295544694260256	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1900	0.12867406790875519	No Hit
GGTGTGAACTGAGTCAAATGCTTAG	1867	0.12643920251876103	No Hit
GTTAAGAGGAGTGCTTAGCGTAAGC	1847	0.12508473864603728	No Hit
CCTCTTAACCTTCCAGCACCGGGCA	1816	0.12298531964331548	No Hit
CCCCCTATACATCATCTTACGATTT	1792	0.121359962996047	No Hit
GCCCAACCCTTGGGACCGACTACAG	1722	0.11661933944151391	No Hit
GATAGGGACCGAACTGTCTCACGAC	1647	0.1115400999187999	No Hit
GGATAGGTAGGAGTCTATGAGATCG	1620	0.10971157369062284	No Hit
GGTATAAGGGAGCTTGACTGCGAGA	1618	0.10957612730335047	No Hit
CTATAACATTGAGCTGTGATGGGGA	1598	0.10822166343062672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATC	100	1.8189894E-12	16.151846	12
GGTATCA	585	0.0	16.069492	1
TAGGCCC	405	0.0	15.24607	4
CGAATCG	110	1.2732926E-11	14.682998	13
ATAGGCC	430	0.0	14.137793	3
GACGCTG	55	1.958145E-4	13.819761	11
CCTTATA	425	0.0	13.632689	2
AATAGGC	440	0.0	13.599675	2
CCCTTAT	430	0.0	13.470514	1
AGTTCGG	120	5.0931703E-11	13.460328	10
TTATACC	445	0.0	13.448694	4
CTGACGT	640	0.0	13.358183	17
CGTTACG	150	0.0	13.300617	16
CCACGCG	50	0.0015004922	13.298813	18
GCGAAAT	360	0.0	13.195952	12
CGCATAG	195	0.0	13.155794	10
CGTAGGA	110	2.752131E-9	12.952511	19
ATCGAAT	125	1.0186341E-10	12.921476	11
AATTCCT	405	0.0	12.901834	16
GTATCAA	1855	0.0	12.694939	1
>>END_MODULE