FastQCFastQC Report
Thu 2 Feb 2017
SRR4062061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476599
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG40380.2734662559029229No Hit
GGCCTATTCACTGCGGCTGACGTAAAGTCAGCACCCCTTCTCCCGAAGTT32720.22159028957760368No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC30010.203237304102197No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA26500.1794664631358954No Hit
CGTTGAGACAGTGCCCAAATCATTACGCCTTTCGTGCGGGTCGGAACTTA25590.17330365251500238No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC25320.17147512628682532No Hit
TTCCAGCACCGGGCAGGCGTCACCCCCTATACATCATCTTACGATTTAGC23830.16138437043503348No Hit
GTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT21530.1458080358987105No Hit
GAATAGGCCCAAGCAACTGTTTATCAAAAACACAGCTCTCTGCTAAATCG21110.14296366176599062No Hit
TCCCTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCA21080.14276049218508208No Hit
GTGCTGGAAGGTTAAGAGGAGTGCTTAGCGTAAGCGAAGGTATGAATTGA20640.13978067166508984No Hit
CTCTAACCCAGATAGGTGATCCCTATCGGAGACAGTGTCTGACGGGCAGT19780.13395647701237776No Hit
GTGATGGGGAGCGAAGTTTAGTAGCGAAGTTAGTGACGTCACACTGCCAA19610.13280518272056258No Hit
GTCTCAACGAGAGACTCGGTGAAATTTTAGTACCTGTGAAGATGCAGGTT19500.13206022759056452No Hit
GTCTCACGACGTTCTGAACCCAGCTCGCGTGCCGCTTTAATGGGCGAACA19190.12996080858784276No Hit
GTACTAGAGTATGTAGTGATGGAGGGACGCAGTAGGCTAACTAAAGCAGA19100.12935129984511706No Hit
GTTAAGAGGAGTGCTTAGCGTAAGCGAAGGTATGAATTGAAGCCCCAGTA18820.12745505042330382No Hit
GTATAGGGGGTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGCTTA17900.12122451660877462No Hit
CCCCCTATACATCATCTTACGATTTAGCAGAGAGCTGTGTTTTTGATAAA17300.11716112499060341No Hit
CCTCTTAACCTTCCAGCACCGGGCAGGCGTCACCCCCTATACATCATCTT17290.11709340179696721No Hit
GTTCTATAACATTGAGCTGTGATGGGGAGCGAAGTTTAGTAGCGAAGTTA17240.11675478582878628No Hit
GCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGACGAGCCGACATC16540.1120141622742532No Hit
GGATAGGTAGGAGTCTATGAGATCGGGACGCCAGTTTCGAAGGAGACGTT16300.11038880562698472No Hit
GGTGTGAACTGAGTCAAATGCTTAGTTCTATAACATTGAGCTGTGATGGG16070.1088311721733524No Hit
GGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAG15460.104700057361545No Hit
CTATAACATTGAGCTGTGATGGGGAGCGAAGTTTAGTAGCGAAGTTAGTG15260.10334559348882127No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTGCCGC14820.10036577296882905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8500.031.3262061
GTATCAA20950.024.0543041
CGACCCG6400.023.03208437
TAACGTG1407.2759576E-1222.00005126
ATATACC703.2175987E-521.9985623
TTGTCGG7050.021.84402322
TCGGGTA7200.021.69449425
GTCGGGT7300.021.3973124
TGTCGGG7300.021.0959423
CGCACGA7000.020.7471242
CCGCACG6800.020.70943641
GGTATAA10950.020.692718
GTGTAAA12850.020.5503621
ACCCGCA7400.020.5163439
GCCTACG654.923794E-420.3132421
CCCGCAC7150.020.3104940
TAAACCG654.935052E-420.3063645
CACGAAA7650.020.13553844
TGTAAAG12750.020.0150552
CGAATCG1101.2669807E-719.99869213
Produced by FastQC (version 0.11.5)