##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062061_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1476599
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.330216260474238	32.0	32.0	32.0	32.0	32.0
2	31.517402490452724	32.0	32.0	32.0	32.0	32.0
3	31.620144670286244	32.0	32.0	32.0	32.0	32.0
4	31.67835614137623	32.0	32.0	32.0	32.0	32.0
5	31.664031331458304	32.0	32.0	32.0	32.0	32.0
6	35.316905943996986	36.0	36.0	36.0	36.0	36.0
7	35.32857600472437	36.0	36.0	36.0	36.0	36.0
8	35.284936533209084	36.0	36.0	36.0	36.0	36.0
9	35.35385639567682	36.0	36.0	36.0	36.0	36.0
10	35.26339310808147	36.0	36.0	36.0	36.0	36.0
11	35.35343244848466	36.0	36.0	36.0	36.0	36.0
12	35.29764614495879	36.0	36.0	36.0	36.0	36.0
13	35.31569979391832	36.0	36.0	36.0	36.0	36.0
14	35.28598624271044	36.0	36.0	36.0	36.0	36.0
15	35.27086365357148	36.0	36.0	36.0	36.0	36.0
16	35.29660320777679	36.0	36.0	36.0	36.0	36.0
17	35.27618398766354	36.0	36.0	36.0	36.0	36.0
18	35.27706371194888	36.0	36.0	36.0	36.0	36.0
19	35.26346489466673	36.0	36.0	36.0	36.0	36.0
20	35.26376084502292	36.0	36.0	36.0	36.0	36.0
21	35.253567149916805	36.0	36.0	36.0	36.0	36.0
22	35.23947259885724	36.0	36.0	36.0	36.0	36.0
23	35.230711926528464	36.0	36.0	36.0	36.0	36.0
24	35.20489652234628	36.0	36.0	36.0	36.0	36.0
25	35.187790320865716	36.0	36.0	36.0	36.0	36.0
26	35.15751873054228	36.0	36.0	36.0	36.0	36.0
27	35.143530504896724	36.0	36.0	36.0	36.0	36.0
28	35.14156924120902	36.0	36.0	36.0	36.0	36.0
29	35.12912849053805	36.0	36.0	36.0	36.0	36.0
30	35.113466147545815	36.0	36.0	36.0	36.0	36.0
31	35.122246459600746	36.0	36.0	36.0	36.0	36.0
32	35.08588790863328	36.0	36.0	36.0	36.0	36.0
33	35.065969840152945	36.0	36.0	36.0	36.0	36.0
34	35.06588315446509	36.0	36.0	36.0	36.0	36.0
35	35.045707737848936	36.0	36.0	36.0	36.0	36.0
36	35.04195587292149	36.0	36.0	36.0	36.0	36.0
37	35.03104972981832	36.0	36.0	36.0	36.0	36.0
38	35.018848042020885	36.0	36.0	36.0	36.0	36.0
39	35.00652309801104	36.0	36.0	36.0	36.0	36.0
40	34.995382632657886	36.0	36.0	36.0	36.0	36.0
41	34.97432275113284	36.0	36.0	36.0	36.0	36.0
42	34.95386560603116	36.0	36.0	36.0	36.0	36.0
43	34.9428646504569	36.0	36.0	36.0	36.0	36.0
44	34.91494102325682	36.0	36.0	36.0	36.0	36.0
45	34.897617430324686	36.0	36.0	36.0	36.0	36.0
46	34.87558368927515	36.0	36.0	36.0	36.0	36.0
47	34.84398540158838	36.0	36.0	36.0	36.0	36.0
48	34.82001071380923	36.0	36.0	36.0	36.0	36.0
49	34.80688121825899	36.0	36.0	36.0	36.0	36.0
50	34.42849751354294	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	12.0
21	37.0
22	75.0
23	224.0
24	570.0
25	1390.0
26	2963.0
27	5699.0
28	9833.0
29	15853.0
30	24310.0
31	35011.0
32	53575.0
33	90843.0
34	212681.0
35	1023520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76639231815124	18.83953897526121	12.288437866463248	26.1056308401243
2	16.077213100653758	19.264197239893075	39.06431061327959	25.59427904617358
3	18.582537132702512	23.189145846257205	29.875267168541704	28.353049852498586
4	12.427680094595758	16.145480255641512	38.0945673131297	33.33227233663303
5	13.439735500294933	37.156533358074874	34.655109477928676	14.748621663701519
6	32.19980278965946	38.26849050112556	16.368932328138502	13.162774381076476
7	29.179418379668416	31.54485408699315	19.940078518270703	19.335649015067734
8	27.159980468630955	35.12104505014564	18.53766662445254	19.181307856770864
9	27.540923432834507	15.044301127117112	19.003331303894964	38.41144413615341
10	16.293872807629427	27.616656666201635	31.6172715932163	24.472198932952637
11	36.29360442476258	22.547150580489355	22.766980067032417	18.392264927715647
12	22.633294482794582	25.21029744703877	29.96873220149817	22.187675868668475
13	29.077088634084134	20.567669353697244	25.947328963381395	24.407913048837226
14	22.25275785775285	20.91637607772997	25.630384417164038	31.20048164735314
15	25.020604781663806	28.197499795137336	23.086159478639768	23.695735944559086
16	25.049658300775228	27.974367986802033	23.45903503407511	23.516938678347625
17	22.813302731479567	27.464057608057434	25.52778377880521	24.19485588165778
18	22.960142164624358	27.41355467093955	26.89601367467652	22.73028948975957
19	25.863352203272523	26.06895981915198	25.736642107979215	22.33104586959628
20	23.606321566630275	26.34925192808324	26.71177492015419	23.33265158513229
21	25.988640111499468	24.10302323108711	25.863352203272523	24.0449844541409
22	24.210025877032287	25.563135285883305	26.218357184313412	24.008481652770996
23	23.797930243756092	26.290414662342315	27.083859598983885	22.827795494917712
24	24.227760986006206	26.793414254396808	25.233529032909086	23.7452957266879
25	23.380714929570978	26.35194667724959	25.89501643309971	24.372321960079724
26	24.34724778781439	25.43116117226536	26.29097775014662	23.93061328977363
27	25.498325539501355	25.6326209962786	25.210162569966716	23.658890894253332
28	23.819956561696408	26.26080454998283	26.41304855757607	23.50619033074469
29	24.85285363380923	25.62747571941045	25.478009821270753	24.04166082550957
30	23.00213466831372	27.453514075807817	27.36655614069306	22.177795115185404
31	24.87118987506366	24.957198738717263	26.967638995741545	23.20397239047753
32	23.777793730427906	26.408987723081257	25.943591730146174	23.869626816344663
33	23.97258557695238	25.962501566103096	25.519252063023373	24.54566079392115
34	23.870601158621735	25.286777165101565	26.48345977399156	24.35916190228514
35	24.140436802829214	25.565744927681493	26.56751047851371	23.726307790975575
36	23.87453829556398	26.260231316123456	26.173138994198812	23.692091394113753
37	24.734638924049133	24.872932120525025	26.100030272800602	24.29239868262524
38	24.19593945275596	25.906220984844225	26.861185738308098	23.03665382409171
39	25.52118570891618	25.797498958746022	25.347135451057678	23.334179881280114
40	24.137036620664528	26.266526163519117	26.044123044999385	23.552314170816974
41	24.101507262709063	26.151644606740966	26.07085001598284	23.675998114567136
42	24.81669761778016	26.498284482046326	24.951135167643965	23.73388273252955
43	24.713178483577693	25.388614371010913	25.68586576075767	24.21234138465373
44	23.90777257938239	25.645299365265988	26.18027204434198	24.26665601100964
45	24.11883264118589	26.224261190168523	26.022305839773963	23.634600328871624
46	24.456204463600002	26.632402479807876	25.675587816596074	23.235805239996044
47	23.685714711407833	26.17314852815489	26.013387675049536	24.12774908538774
48	23.86311456424302	26.78996894077617	25.693753073075186	23.653163421905624
49	24.525728279943408	25.87443372183509	25.99376780783555	23.606070190385953
50	23.515667698109066	26.80212898380236	25.596431737543625	24.08577158054495
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	39.5
2	36.0
3	114.5
4	193.0
5	241.5
6	290.0
7	237.0
8	184.0
9	251.5
10	319.0
11	523.0
12	727.0
13	1478.0
14	2229.0
15	2856.0
16	3483.0
17	3301.5
18	3120.0
19	2937.0
20	2754.0
21	3334.5
22	3915.0
23	4895.5
24	5876.0
25	7591.5
26	9307.0
27	11807.5
28	14308.0
29	17436.0
30	20564.0
31	24438.5
32	28313.0
33	33955.0
34	39597.0
35	43675.5
36	47754.0
37	55905.0
38	64056.0
39	70225.5
40	76395.0
41	85207.0
42	94019.0
43	99685.0
44	105351.0
45	109012.0
46	112673.0
47	112068.0
48	111463.0
49	115873.0
50	120283.0
51	125484.0
52	130685.0
53	122457.5
54	114230.0
55	106248.0
56	98266.0
57	88240.0
58	78214.0
59	73261.0
60	68308.0
61	56746.0
62	45184.0
63	37328.0
64	29472.0
65	25064.5
66	20657.0
67	15241.5
68	9826.0
69	7970.0
70	6114.0
71	4782.5
72	3451.0
73	2739.5
74	2028.0
75	1535.5
76	1043.0
77	908.0
78	773.0
79	653.0
80	533.0
81	407.0
82	281.0
83	198.5
84	116.0
85	97.5
86	79.0
87	57.0
88	35.0
89	28.5
90	22.0
91	15.0
92	8.0
93	10.5
94	13.0
95	9.0
96	5.0
97	3.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033590704043548726
2	0.004198838005443591
3	2.0316958090856082E-4
4	0.0
5	0.0
6	2.0316958090856082E-4
7	0.0
8	0.0
9	0.0
10	6.772319363618693E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.417855490894955E-4
17	0.0
18	6.772319363618693E-5
19	0.0
20	2.0316958090856082E-4
21	0.0
22	0.0
23	0.0
24	0.001083571098178991
25	4.0633916181712163E-4
26	3.3861596818093474E-4
27	2.7089277454474774E-4
28	0.0014899102599961129
29	5.417855490894955E-4
30	0.0015576334536322996
31	4.740623554533086E-4
32	4.740623554533086E-4
33	2.7089277454474774E-4
34	0.001151294291815178
35	4.0633916181712163E-4
36	3.3861596818093474E-4
37	0.0017608030345408604
38	0.0
39	9.481247109066172E-4
40	6.772319363618693E-5
41	0.001015847904542804
42	0.0052146859099863946
43	0.004808346748169273
44	0.005079239522714021
45	0.002370311777266543
46	0.0022348653899941693
47	0.001151294291815178
48	0.004402007586352151
49	0.004876069941805459
50	0.0017608030345408604
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1476599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.876101061636657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.38173896596211	18.643526745904097
2	16.1907059483894	9.998117462434257
3	7.806122503483226	7.230678819511926
4	4.45065578064513	5.496755906950257
5	2.8046076614255644	4.329767479620808
6	1.87001315161239	3.46432290334643
7	1.3146953033663684	2.841486553639934
8	0.907947456601662	2.2427101942951095
9	0.6877484654868663	1.9111492012822213
>10	3.1701685958827284	16.68607541003386
>50	0.20222853708812996	4.361749022268331
>100	0.17703568807096073	11.582571895081282
>500	0.024441488244797298	5.125486621056046
>1k	0.011890453740712197	6.085601784575306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAAGGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGG	4038	0.2734662559029229	No Hit
GGCCTATTCACTGCGGCTGACGTAAAGTCAGCACCCCTTCTCCCGAAGTT	3272	0.22159028957760368	No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGGGC	3001	0.203237304102197	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA	2650	0.1794664631358954	No Hit
CGTTGAGACAGTGCCCAAATCATTACGCCTTTCGTGCGGGTCGGAACTTA	2559	0.17330365251500238	No Hit
CCCTTATACCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAAC	2532	0.17147512628682532	No Hit
TTCCAGCACCGGGCAGGCGTCACCCCCTATACATCATCTTACGATTTAGC	2383	0.16138437043503348	No Hit
GTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAGT	2153	0.1458080358987105	No Hit
GAATAGGCCCAAGCAACTGTTTATCAAAAACACAGCTCTCTGCTAAATCG	2111	0.14296366176599062	No Hit
TCCCTACATAGCTACCCAGCGATGCCTTTGGCAAGACAACTGGTACACCA	2108	0.14276049218508208	No Hit
GTGCTGGAAGGTTAAGAGGAGTGCTTAGCGTAAGCGAAGGTATGAATTGA	2064	0.13978067166508984	No Hit
CTCTAACCCAGATAGGTGATCCCTATCGGAGACAGTGTCTGACGGGCAGT	1978	0.13395647701237776	No Hit
GTGATGGGGAGCGAAGTTTAGTAGCGAAGTTAGTGACGTCACACTGCCAA	1961	0.13280518272056258	No Hit
GTCTCAACGAGAGACTCGGTGAAATTTTAGTACCTGTGAAGATGCAGGTT	1950	0.13206022759056452	No Hit
GTCTCACGACGTTCTGAACCCAGCTCGCGTGCCGCTTTAATGGGCGAACA	1919	0.12996080858784276	No Hit
GTACTAGAGTATGTAGTGATGGAGGGACGCAGTAGGCTAACTAAAGCAGA	1910	0.12935129984511706	No Hit
GTTAAGAGGAGTGCTTAGCGTAAGCGAAGGTATGAATTGAAGCCCCAGTA	1882	0.12745505042330382	No Hit
GTATAGGGGGTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGAGTGCTTA	1790	0.12122451660877462	No Hit
CCCCCTATACATCATCTTACGATTTAGCAGAGAGCTGTGTTTTTGATAAA	1730	0.11716112499060341	No Hit
CCTCTTAACCTTCCAGCACCGGGCAGGCGTCACCCCCTATACATCATCTT	1729	0.11709340179696721	No Hit
GTTCTATAACATTGAGCTGTGATGGGGAGCGAAGTTTAGTAGCGAAGTTA	1724	0.11675478582878628	No Hit
GCCCAACCCTTGGGACCGACTACAGCCCCAGGATGCGACGAGCCGACATC	1654	0.1120141622742532	No Hit
GGATAGGTAGGAGTCTATGAGATCGGGACGCCAGTTTCGAAGGAGACGTT	1630	0.11038880562698472	No Hit
GGTGTGAACTGAGTCAAATGCTTAGTTCTATAACATTGAGCTGTGATGGG	1607	0.1088311721733524	No Hit
GGTATAAGGGAGCTTGACTGCGAGAGCTACAACTCGAGCAGGGACGAAAG	1546	0.104700057361545	No Hit
CTATAACATTGAGCTGTGATGGGGAGCGAAGTTTAGTAGCGAAGTTAGTG	1526	0.10334559348882127	No Hit
GATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCGCGTGCCGC	1482	0.10036577296882905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	6.772319363618693E-5	0.0
16	0.0	0.0	0.0	2.0316958090856082E-4	0.0
17	0.0	0.0	0.0	2.0316958090856082E-4	0.0
18	0.0	0.0	0.0	2.0316958090856082E-4	0.0
19	0.0	0.0	0.0	2.0316958090856082E-4	0.0
20	0.0	0.0	0.0	3.386159681809347E-4	0.0
21	0.0	0.0	0.0	5.417855490894955E-4	0.0
22	0.0	0.0	0.0	8.804015172704302E-4	0.0
23	0.0	0.0	0.0	0.0013544638727237387	0.0
24	0.0	0.0	0.0	0.0019639726154494215	0.0
25	0.0	0.0	0.0	0.0026412045518112905	0.0
26	0.0	0.0	0.0	0.003250713294536973	0.0
27	0.0	0.0	0.0	0.0037924988436264686	0.0
28	0.0	0.0	0.0	0.004876069941805459	0.0
29	0.0	0.0	0.0	0.006907765750891068	0.0
30	0.0	0.0	0.0	0.010226202239064229	0.0
31	0.0	0.0	0.0	0.01679535202177436	0.0
32	0.0	0.0	0.0	0.02526075122629773	0.0
33	0.0	0.0	0.0	0.03237168655809736	0.0
34	0.0	0.0	0.0	0.04192065686079972	0.0
35	0.0	0.0	0.0	0.05295953742349819	0.0
36	0.0	0.0	0.0	0.0658946674080099	0.0
37	0.0	0.0	0.0	0.08946233879340294	0.0
38	0.0	0.0	0.0	0.121359962996047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	850	0.0	31.326206	1
GTATCAA	2095	0.0	24.054304	1
CGACCCG	640	0.0	23.032084	37
TAACGTG	140	7.2759576E-12	22.000051	26
ATATACC	70	3.2175987E-5	21.998562	3
TTGTCGG	705	0.0	21.844023	22
TCGGGTA	720	0.0	21.694494	25
GTCGGGT	730	0.0	21.39731	24
TGTCGGG	730	0.0	21.09594	23
CGCACGA	700	0.0	20.74712	42
CCGCACG	680	0.0	20.709436	41
GGTATAA	1095	0.0	20.69271	8
GTGTAAA	1285	0.0	20.550362	1
ACCCGCA	740	0.0	20.51634	39
GCCTACG	65	4.923794E-4	20.313242	1
CCCGCAC	715	0.0	20.31049	40
TAAACCG	65	4.935052E-4	20.306364	5
CACGAAA	765	0.0	20.135538	44
TGTAAAG	1275	0.0	20.015055	2
CGAATCG	110	1.2669807E-7	19.998692	13
>>END_MODULE