FastQCFastQC Report
Thu 2 Feb 2017
SRR4062060_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062060_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332256
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16470.4957021092169893No Hit
CCATAGGGTCTTCTCGTCTTATTAT12140.36538091110469034No Hit
TATCAACGCAGAGTACTTTTTTTTT10380.3124097081768275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9540.2871279976885293No Hit
GTCCTAAAGTGTGTATTTCTCATTT8820.2654579601271309No Hit
CTGTAGGACGTGGAATATGGCAAGA8020.24138014061446597No Hit
GTATTAGAGGCACTGCCTGCCCAGT7810.2350597129923914No Hit
CTTTAGGACGTGAAATATGGCGAGG6980.21007897524800154No Hit
GTCCTACAGTGGACATTTCTAAATT6940.20887508427236828No Hit
GATATACACTGTTCTACAAATCCCG6690.20135076567466048No Hit
GGTATCAACGCAGAGTACTTTTTTT6560.19743812000385247No Hit
GTCAGGATACCGCGGCCGTTAAACT6200.18660310122315324No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6100.18359337378407012No Hit
CTCTAATACTTGTAATGCTAGAGGT5720.17215640951555428No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5650.17004960030819607No Hit
GACTATAGGCAATAATCACACTATA5540.16673890012520468No Hit
CCATTGGGATGTCCTGATCCAACAT5510.16583598189347973No Hit
ATAAATAATCCACCTATAACTTCTC5490.1652340364056631No Hit
CCTATAACTTCTCTGTTAACCCAAC5420.1631272271983049No Hit
GTTATATAATTTAAGCTCCATAGGG5390.16222430896658No Hit
GTTCATGCTAGTCCCTAATTAAGGA5390.16222430896658No Hit
ACCTATAACTTCTCTGTTAACCCAA5320.1601174997592218No Hit
GTGTATATCAATGAGTTACAATGAG5190.15620485408841375No Hit
GCCTAAAGGAAAGATCCAAAAAGAT5100.15349609939323894No Hit
CTTCTACACCATTGGGATGTCCTGA5090.15319512664933063No Hit
CTGTTAGTATGAGTAACAAGAATTC5010.15078734469806412No Hit
CACTATAAATAATCCACCTATAACT5010.15078734469806412No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4970.1495834537224309No Hit
ATCTATAACTTTATAGATGCAACAC4780.14386497158817296No Hit
GATTAAACCTTGTACCTTTTGCATA4730.14236010786863143No Hit
TTGTAGAACAGTGTATATCAATGAG4620.13904940768563998No Hit
CTATAGAACTAGTACCGCAAGGGAA4550.1369425984782818No Hit
GGTCAGGATACCGCGGCCGTTAAAC4530.1363406529904652No Hit
GTACATGGGGTGGTATCAACGCAAA4450.13393287103919868No Hit
GATTAAAGATAAGAGACAGTTGGAC4300.12941827988057403No Hit
GTCCTACAGTGTGCATTTCTCATTT4260.12821438890494077No Hit
GTACATGGGAGAAATCGTAAATAGA4240.12761244341712416No Hit
CTGTTAACCCAACACCGGAATGCCT4190.12610757969758257No Hit
GTGTAAGCATCTGGGTAGTCTGAGT4150.12490368872194935No Hit
ATCCTGACCGTGCAAAGGTAGCATA4110.12369979774631609No Hit
CCTCTAGCATTACAAGTATTAGAGG4000.12038909756332467No Hit
GTATCAACGCAGAGTACATGGGAGA4000.12038909756332467No Hit
GTATCCTGACCGTGCAAAGGTAGCA3890.11707839738033324No Hit
GTTATAGATTAACCCAATTTTAAGT3830.11527256091688337No Hit
CTGAAGGACCTGGAATATGGCGAGA3740.11256380622170856No Hit
GTTCTACAGTGTGGTTTTTATCATT3680.1107579697582587No Hit
CTTTATTGGTGGCTGCTTTTAGGCC3650.10985505152653376No Hit
CTATTAAAGGTTTTTTCCGTTCCAG3600.10835018780699221No Hit
GTGTATATCAATGAGTTACAATGAA3570.10744726957526726No Hit
GTATCAACGCAGAGTACATGGGGTG3450.10383559664836753No Hit
GTTAGTATGAGTAACAAGAATTCCA3400.10233073292882597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACTC250.00603666818.9921672
CTAAAAC307.7266525E-418.9921653
GTTGAGC350.0021647916.286368
CTCTAGG350.00217790716.2716451
TCTAGGG508.711666E-515.1937333
ATTATAC508.711666E-515.1937333
GTTCATT456.7250855E-414.78282211
TCATTTC400.005260875414.25271313
GAAACAC602.5516792E-514.25271315
TTGAACT400.005260875414.2527139
AATATTC400.005266141614.2505667
GACTTGG400.005266141614.2505667
AAACCTG400.005266141614.2505666
GGGTTTG400.005266141614.2505667
TTTGTGC400.00528196714.2441243
GTTTAGA400.005297832714.2376881
CTCTTAA801.2916098E-714.2376881
AGAACAG1350.014.0725125
GACATGG954.8457878E-914.0005567
AGGCACT1500.013.9338878
Produced by FastQC (version 0.11.5)