##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062060_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332256
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17525040932293	32.0	32.0	32.0	32.0	32.0
2	30.817252359626313	32.0	32.0	32.0	32.0	32.0
3	30.873940575941443	32.0	32.0	32.0	32.0	32.0
4	30.861359915246076	32.0	32.0	32.0	32.0	32.0
5	30.85398909274776	32.0	32.0	32.0	32.0	32.0
6	34.47367391409034	36.0	36.0	36.0	32.0	36.0
7	34.355388616006934	36.0	36.0	36.0	32.0	36.0
8	34.294589112010016	36.0	36.0	36.0	32.0	36.0
9	34.366009944139456	36.0	36.0	36.0	32.0	36.0
10	34.15334862274872	36.0	36.0	36.0	32.0	36.0
11	34.452343975729555	36.0	36.0	36.0	32.0	36.0
12	34.249295723779255	36.0	36.0	36.0	32.0	36.0
13	34.36205215255706	36.0	36.0	36.0	32.0	36.0
14	34.28998121930078	36.0	36.0	36.0	32.0	36.0
15	34.25314516517384	36.0	36.0	36.0	32.0	36.0
16	34.24725211884812	36.0	36.0	36.0	32.0	36.0
17	34.18281385437735	36.0	36.0	36.0	32.0	36.0
18	34.23192959645574	36.0	36.0	36.0	32.0	36.0
19	34.134534816527015	36.0	36.0	36.0	32.0	36.0
20	34.11008680053934	36.0	36.0	36.0	32.0	36.0
21	34.06114562265241	36.0	36.0	36.0	32.0	36.0
22	34.0361709043629	36.0	36.0	36.0	32.0	36.0
23	34.0565317104883	36.0	36.0	36.0	32.0	36.0
24	34.02513724357122	36.0	36.0	36.0	32.0	36.0
25	33.70433039583935	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	1.0
5	21.0
6	74.0
7	12.0
8	55.0
9	37.0
10	28.0
11	13.0
12	10.0
13	17.0
14	97.0
15	116.0
16	195.0
17	216.0
18	309.0
19	398.0
20	524.0
21	749.0
22	1099.0
23	1414.0
24	2075.0
25	2805.0
26	3678.0
27	4600.0
28	6114.0
29	7970.0
30	10764.0
31	14350.0
32	20947.0
33	30061.0
34	68921.0
35	154584.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91713518654515	22.297533197693316	12.6220459169546	24.16328569880693
2	13.523608302032168	22.540149764037366	42.85881970528749	21.077422228642977
3	17.03847056769821	28.38521131423549	31.26734365876709	23.30897445929921
4	9.88261144387924	19.226740511091712	42.242723414502	28.647924630527044
5	10.338079309690764	41.304446003353995	36.95779804360196	11.399676643353276
6	26.70232437674298	43.015726736656966	19.111207483300507	11.170741403299543
7	25.278331008258785	34.57571657994089	22.898017108129526	17.247935303670804
8	23.47563823280395	38.51512385015653	20.73107899232584	17.278158924713686
9	25.608326578334385	16.2240510105378	22.24057037302661	35.92705203810121
10	14.7317473042126	28.5373733477189	34.74386252539073	21.987016822677766
11	31.246590494675896	24.995403760778558	27.125949010376953	16.632056734168593
12	22.128496990847474	28.20858233806781	31.853619873365457	17.809300797719253
13	25.696118611378978	23.587270973963356	28.78164175506268	21.934968659594986
14	19.817165033565946	25.4893275963891	28.524424196405974	26.169083173638985
15	22.225537352476703	32.61907345123142	24.769447759574696	20.38594143671718
16	20.752653179295258	29.64901496387096	29.293449765869354	20.304882090964426
17	18.136479380433638	30.472359083306966	29.508053458693063	21.883108077566334
18	18.084388389063978	30.043025785341104	32.44499749920155	19.427588326393366
19	21.890401879857805	28.468397903235527	29.03084894860517	20.6103512683015
20	20.94155355131009	30.389020430049225	29.628692440701045	19.040733577939644
21	21.37865177339575	27.724257595748913	28.770762918647314	22.126327712208024
22	21.073324455745627	30.332427498026643	28.74229487650713	19.851953169720595
23	20.763065393987404	30.24701584021831	29.36419249223652	19.62572627355777
24	21.5541678339795	30.09407788311207	29.369892064983926	18.9818622179245
25	20.47310506450748	30.021959352605755	30.713874756383724	18.791060826503042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1062.0
1	1062.0
2	683.5
3	305.0
4	305.0
5	305.0
6	866.5
7	1428.0
8	1428.0
9	1428.0
10	1430.0
11	1432.0
12	1432.0
13	1432.0
14	1797.5
15	2163.0
16	2163.0
17	2163.0
18	3854.0
19	5545.0
20	5545.0
21	5545.0
22	8472.0
23	11399.0
24	11399.0
25	11399.0
26	18504.5
27	25610.0
28	25610.0
29	25610.0
30	32322.0
31	39034.0
32	39034.0
33	39034.0
34	41088.5
35	43143.0
36	43143.0
37	43143.0
38	42522.0
39	41901.0
40	41901.0
41	41901.0
42	39540.5
43	37180.0
44	37180.0
45	37180.0
46	39061.0
47	40942.0
48	40942.0
49	40942.0
50	36186.5
51	31431.0
52	31431.0
53	31431.0
54	25586.0
55	19741.0
56	19741.0
57	19741.0
58	16135.0
59	12529.0
60	12529.0
61	12529.0
62	10048.5
63	7568.0
64	7568.0
65	7568.0
66	5981.5
67	4395.0
68	4395.0
69	4395.0
70	3450.0
71	2505.0
72	2505.0
73	2505.0
74	2018.5
75	1532.0
76	1532.0
77	1532.0
78	1185.5
79	839.0
80	839.0
81	839.0
82	575.5
83	312.0
84	312.0
85	312.0
86	202.0
87	92.0
88	92.0
89	92.0
90	64.0
91	36.0
92	36.0
93	36.0
94	24.0
95	12.0
96	12.0
97	12.0
98	66.0
99	120.0
100	120.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012038909756332467
2	0.0
3	6.019454878166234E-4
4	0.007825291341616103
5	0.03461186554945584
6	0.06320427622074545
7	0.11045699701435038
8	0.10925310603871713
9	0.1212920157950496
10	0.13302995280747376
11	0.1393503804295483
12	0.13092314360011556
13	0.12520466146585765
14	0.11196186073389193
15	0.13363189829529037
16	0.11737937012424154
17	0.12369979774631609
18	0.10895213329480881
19	0.09510738707502649
20	0.08848598670904363
21	0.08848598670904363
22	0.1005248964653761
23	0.07614610420880286
24	0.08938890494076857
25	0.08397139555041895
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	332256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.934616681113358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.93260659267504	19.36669315226813
2	14.284822700937205	8.266517384185688
3	5.931119132071939	5.14843975729558
4	3.1101448973860224	3.5996340171434076
5	1.904573681308965	2.7554054704805933
6	1.2076515805569135	2.096576134065299
7	0.8331859741826769	1.6875541750939034
8	0.6261897084369181	1.449484734662429
9	0.5138500265246472	1.3381248194163535
>10	3.835151918616141	22.520887508427236
>50	0.48264455932679406	9.538428199942214
>100	0.3099743074986738	16.58961764422614
>500	0.024964373758282452	4.4691442742945195
>1k	0.0031205467197853065	1.173492728498507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1647	0.4957021092169893	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1214	0.36538091110469034	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1038	0.3124097081768275	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	954	0.2871279976885293	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	882	0.2654579601271309	No Hit
CTGTAGGACGTGGAATATGGCAAGA	802	0.24138014061446597	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	781	0.2350597129923914	No Hit
CTTTAGGACGTGAAATATGGCGAGG	698	0.21007897524800154	No Hit
GTCCTACAGTGGACATTTCTAAATT	694	0.20887508427236828	No Hit
GATATACACTGTTCTACAAATCCCG	669	0.20135076567466048	No Hit
GGTATCAACGCAGAGTACTTTTTTT	656	0.19743812000385247	No Hit
GTCAGGATACCGCGGCCGTTAAACT	620	0.18660310122315324	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	610	0.18359337378407012	No Hit
CTCTAATACTTGTAATGCTAGAGGT	572	0.17215640951555428	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	565	0.17004960030819607	No Hit
GACTATAGGCAATAATCACACTATA	554	0.16673890012520468	No Hit
CCATTGGGATGTCCTGATCCAACAT	551	0.16583598189347973	No Hit
ATAAATAATCCACCTATAACTTCTC	549	0.1652340364056631	No Hit
CCTATAACTTCTCTGTTAACCCAAC	542	0.1631272271983049	No Hit
GTTATATAATTTAAGCTCCATAGGG	539	0.16222430896658	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	539	0.16222430896658	No Hit
ACCTATAACTTCTCTGTTAACCCAA	532	0.1601174997592218	No Hit
GTGTATATCAATGAGTTACAATGAG	519	0.15620485408841375	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	510	0.15349609939323894	No Hit
CTTCTACACCATTGGGATGTCCTGA	509	0.15319512664933063	No Hit
CTGTTAGTATGAGTAACAAGAATTC	501	0.15078734469806412	No Hit
CACTATAAATAATCCACCTATAACT	501	0.15078734469806412	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	497	0.1495834537224309	No Hit
ATCTATAACTTTATAGATGCAACAC	478	0.14386497158817296	No Hit
GATTAAACCTTGTACCTTTTGCATA	473	0.14236010786863143	No Hit
TTGTAGAACAGTGTATATCAATGAG	462	0.13904940768563998	No Hit
CTATAGAACTAGTACCGCAAGGGAA	455	0.1369425984782818	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	453	0.1363406529904652	No Hit
GTACATGGGGTGGTATCAACGCAAA	445	0.13393287103919868	No Hit
GATTAAAGATAAGAGACAGTTGGAC	430	0.12941827988057403	No Hit
GTCCTACAGTGTGCATTTCTCATTT	426	0.12821438890494077	No Hit
GTACATGGGAGAAATCGTAAATAGA	424	0.12761244341712416	No Hit
CTGTTAACCCAACACCGGAATGCCT	419	0.12610757969758257	No Hit
GTGTAAGCATCTGGGTAGTCTGAGT	415	0.12490368872194935	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	411	0.12369979774631609	No Hit
CCTCTAGCATTACAAGTATTAGAGG	400	0.12038909756332467	No Hit
GTATCAACGCAGAGTACATGGGAGA	400	0.12038909756332467	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	389	0.11707839738033324	No Hit
GTTATAGATTAACCCAATTTTAAGT	383	0.11527256091688337	No Hit
CTGAAGGACCTGGAATATGGCGAGA	374	0.11256380622170856	No Hit
GTTCTACAGTGTGGTTTTTATCATT	368	0.1107579697582587	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	365	0.10985505152653376	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	360	0.10835018780699221	No Hit
GTGTATATCAATGAGTTACAATGAA	357	0.10744726957526726	No Hit
GTATCAACGCAGAGTACATGGGGTG	345	0.10383559664836753	No Hit
GTTAGTATGAGTAACAAGAATTCCA	340	0.10233073292882597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.009727439083117E-4	0.0
8	0.0	0.0	0.0	6.019454878166234E-4	0.0
9	0.0	0.0	0.0	6.019454878166234E-4	0.0
10	0.0	0.0	0.0	9.02918231724935E-4	0.0
11	0.0	0.0	0.0	9.02918231724935E-4	0.0
12	0.0	0.0	0.0	9.02918231724935E-4	0.0
13	0.0	0.0	0.0	9.02918231724935E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCACTC	25	0.006036668	18.992167	2
CTAAAAC	30	7.7266525E-4	18.992165	3
GTTGAGC	35	0.00216479	16.28636	8
CTCTAGG	35	0.002177907	16.271645	1
TCTAGGG	50	8.711666E-5	15.193733	3
ATTATAC	50	8.711666E-5	15.193733	3
GTTCATT	45	6.7250855E-4	14.782822	11
TCATTTC	40	0.0052608754	14.252713	13
GAAACAC	60	2.5516792E-5	14.252713	15
TTGAACT	40	0.0052608754	14.252713	9
AATATTC	40	0.0052661416	14.250566	7
GACTTGG	40	0.0052661416	14.250566	7
AAACCTG	40	0.0052661416	14.250566	6
GGGTTTG	40	0.0052661416	14.250566	7
TTTGTGC	40	0.005281967	14.244124	3
GTTTAGA	40	0.0052978327	14.237688	1
CTCTTAA	80	1.2916098E-7	14.237688	1
AGAACAG	135	0.0	14.072512	5
GACATGG	95	4.8457878E-9	14.000556	7
AGGCACT	150	0.0	13.933887	8
>>END_MODULE