##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	332256
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166344625830686	32.0	32.0	32.0	32.0	32.0
2	31.463516083983436	32.0	32.0	32.0	32.0	32.0
3	31.59804488105557	32.0	32.0	32.0	32.0	32.0
4	31.68897777617259	32.0	32.0	32.0	32.0	32.0
5	31.684758138302996	32.0	32.0	32.0	32.0	32.0
6	35.307103558701726	36.0	36.0	36.0	36.0	36.0
7	35.315762544543965	36.0	36.0	36.0	36.0	36.0
8	35.284786429740926	36.0	36.0	36.0	36.0	36.0
9	35.34016240489261	36.0	36.0	36.0	36.0	36.0
10	35.24936494751035	36.0	36.0	36.0	36.0	36.0
11	35.35835620726187	36.0	36.0	36.0	36.0	36.0
12	35.27653375710295	36.0	36.0	36.0	36.0	36.0
13	35.32620328903015	36.0	36.0	36.0	36.0	36.0
14	35.285394394683614	36.0	36.0	36.0	36.0	36.0
15	35.28380224886834	36.0	36.0	36.0	36.0	36.0
16	35.28525895694886	36.0	36.0	36.0	36.0	36.0
17	35.274511220263896	36.0	36.0	36.0	36.0	36.0
18	35.28968325628431	36.0	36.0	36.0	36.0	36.0
19	35.23422601849177	36.0	36.0	36.0	36.0	36.0
20	35.211845685254744	36.0	36.0	36.0	36.0	36.0
21	35.18194103342001	36.0	36.0	36.0	36.0	36.0
22	35.17187349513628	36.0	36.0	36.0	36.0	36.0
23	35.165826952711164	36.0	36.0	36.0	36.0	36.0
24	35.157435832611	36.0	36.0	36.0	36.0	36.0
25	35.13609987479534	36.0	36.0	36.0	36.0	36.0
26	35.112235745930846	36.0	36.0	36.0	36.0	36.0
27	35.07544182798806	36.0	36.0	36.0	36.0	36.0
28	35.0417539487624	36.0	36.0	36.0	36.0	36.0
29	35.0225940238852	36.0	36.0	36.0	36.0	36.0
30	34.96424443802369	36.0	36.0	36.0	36.0	36.0
31	34.98818381007416	36.0	36.0	36.0	36.0	36.0
32	34.94048564961957	36.0	36.0	36.0	36.0	36.0
33	34.927528772994314	36.0	36.0	36.0	36.0	36.0
34	34.89468060772416	36.0	36.0	36.0	36.0	36.0
35	34.885714629683136	36.0	36.0	36.0	36.0	36.0
36	34.829631368583264	36.0	36.0	36.0	36.0	36.0
37	34.781325243185975	36.0	36.0	36.0	32.0	36.0
38	34.75039427429452	36.0	36.0	36.0	32.0	36.0
39	34.66518889049408	36.0	36.0	36.0	32.0	36.0
40	34.673724477511314	36.0	36.0	36.0	32.0	36.0
41	34.636211234710586	36.0	36.0	36.0	32.0	36.0
42	34.61182341327169	36.0	36.0	36.0	32.0	36.0
43	34.63287344698064	36.0	36.0	36.0	32.0	36.0
44	34.51892516613695	36.0	36.0	36.0	32.0	36.0
45	34.469291750939036	36.0	36.0	36.0	32.0	36.0
46	34.31985878358856	36.0	36.0	36.0	32.0	36.0
47	34.2972677694308	36.0	36.0	36.0	32.0	36.0
48	34.24433870268709	36.0	36.0	36.0	32.0	36.0
49	34.191960416064724	36.0	36.0	36.0	32.0	36.0
50	33.82389482808437	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	4.0
21	6.0
22	16.0
23	55.0
24	119.0
25	359.0
26	786.0
27	1719.0
28	2852.0
29	4620.0
30	6881.0
31	9969.0
32	14840.0
33	23612.0
34	52989.0
35	213425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.41995911473465	22.860222370080898	13.016447437746312	24.70337107743814
2	13.061380512221623	22.76403432468795	42.63135875077881	21.54322641231162
3	16.786152845998267	28.425671771164403	31.73125541750939	23.05691996532794
4	9.75633246653183	19.21169218915535	42.588546181257826	28.443429163054994
5	10.108771549648464	41.36208224983145	36.903772994317634	11.625373206202447
6	26.764001252046615	43.04451988827891	18.92275835500337	11.268720504671096
7	25.03732062024463	34.42074785707406	23.18874602715978	17.353185495521526
8	23.403941539054223	38.32135461812578	20.942887412115958	17.331816430704038
9	25.457478570740633	15.637941827988058	22.57957719348936	36.32500240778195
10	14.566177886930559	28.402496869883464	35.02419820861023	22.00712703457575
11	31.26565058268323	24.644250216700375	27.352402966387363	16.737696234229027
12	22.066719637869596	27.9221443706058	32.17669507849369	17.834440913030917
13	25.788548589039777	23.23870750264856	28.970733410382355	22.00201049792931
14	19.796482230569197	25.17155446402774	28.876528941539053	26.15543436386401
15	22.300575459886353	32.452085139169796	24.90910623133969	20.338233169604162
16	20.9713682043503	29.184988547883687	29.3959723463746	20.447670901391408
17	18.070403544255033	30.189973995954926	29.75416546277569	21.98545699701435
18	18.196511123952614	29.234987479533853	32.899932582105365	19.668568814408165
19	21.963786959452953	27.915522970239813	29.615718000577868	20.504972069729366
20	20.870653953577964	29.967254165462776	30.117138591929116	19.044953289030143
21	21.49366753346817	27.226897332177597	29.22686121544833	22.052573918905903
22	20.895333718578446	29.86462245979004	29.411357507464125	19.828686314167392
23	20.81256621400366	29.589232399113936	29.858903977655782	19.73929740922662
24	21.614186736653284	29.618906017685266	29.616197246684766	19.150709998976687
25	20.775007148124182	29.248017336082228	31.209763585198115	18.767211930595476
26	18.22420061639218	29.782155927959163	33.008282769912356	18.9853606857363
27	18.790085927977003	30.73934177062798	30.27433748175347	20.19623481964154
28	18.515218915819666	29.267932978380802	31.381094413560835	20.835753692238697
29	19.732976981604548	28.60595444476548	29.587125589906577	22.073942983723395
30	19.682290302871607	28.697408300301277	30.917704279570085	20.702597117257028
31	20.710360146153246	27.385072865939915	31.09067159462339	20.813895393283453
32	19.060962212758273	29.820168244270214	30.482611247385293	20.63625829558622
33	19.58489839160166	30.13549792930752	30.822919676394108	19.45668400269672
34	19.93944428392565	29.396910815756527	31.912441009342196	18.751203890975635
35	20.0210078975248	30.483121448521622	30.137303765770973	19.358566888182608
36	18.540221997495905	29.375240778195128	31.113960319753442	20.970576904555525
37	20.7441941658741	28.887711736874422	28.572589480274008	21.795504616977475
38	20.019503033805258	30.417810363093515	29.714136087835886	19.848550515265337
39	20.341788762746347	29.919458724100984	28.6628823904747	21.075870122677966
40	21.37056968120967	29.281939227583546	30.772958200905325	18.574532890301455
41	20.700604353269767	28.534021958971394	31.58708947317731	19.178284214581527
42	20.251983482019238	31.192737867350502	29.44492601822757	19.11035263240269
43	18.36549806168886	30.988899857937447	30.732464905733064	19.91313717464063
44	18.932943255046787	30.672313170258935	29.746178226046755	20.64856534864752
45	19.248457486832205	30.32987208427389	30.520692249811887	19.900978179082017
46	21.060710491227965	29.087521708116505	28.27578111597025	21.57598668468528
47	21.422465944729034	30.095047764661974	28.359327502452942	20.123158788156047
48	19.81261267768839	30.812964818978312	27.63526589677144	21.739156606561867
49	20.11876884921232	29.765652747093984	29.428850055080336	20.686728348613357
50	18.917096808174445	30.541903056387415	28.693322899580142	21.847677235858
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	538.0
1	296.5
2	55.0
3	154.5
4	254.0
5	234.0
6	214.0
7	149.0
8	84.0
9	147.0
10	210.0
11	356.5
12	503.0
13	867.0
14	1231.0
15	1600.5
16	1970.0
17	2175.5
18	2381.0
19	2461.5
20	2542.0
21	2938.0
22	3334.0
23	3169.0
24	3004.0
25	4380.5
26	5757.0
27	8492.5
28	11228.0
29	13269.5
30	15311.0
31	15962.5
32	16614.0
33	19299.5
34	21985.0
35	24988.0
36	27991.0
37	31431.0
38	34871.0
39	33502.0
40	32133.0
41	31117.0
42	30101.0
43	24866.5
44	19632.0
45	18417.0
46	17202.0
47	15991.5
48	14781.0
49	13178.0
50	11575.0
51	11552.0
52	11529.0
53	10221.5
54	8914.0
55	8496.0
56	8078.0
57	7911.5
58	7745.0
59	6704.5
60	5664.0
61	5012.0
62	4360.0
63	3596.5
64	2833.0
65	2506.5
66	2180.0
67	1909.5
68	1639.0
69	1483.5
70	1328.0
71	1079.0
72	830.0
73	761.5
74	693.0
75	545.0
76	397.0
77	328.5
78	260.0
79	212.5
80	165.0
81	115.5
82	66.0
83	53.0
84	40.0
85	29.0
86	18.0
87	12.0
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	1.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03220408359818935
2	0.005116536646441299
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.029182317249351E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	6.019454878166234E-4
25	3.009727439083117E-4
26	0.0
27	3.009727439083117E-4
28	0.0015048637195415582
29	0.0
30	9.029182317249351E-4
31	6.019454878166234E-4
32	3.009727439083117E-4
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0012038909756332467
38	0.0
39	0.0012038909756332467
40	0.0
41	0.0
42	0.0036116729268997404
43	0.0024077819512664934
44	0.004514591158624675
45	0.0018058364634498702
46	0.0021068092073581817
47	6.019454878166234E-4
48	9.029182317249351E-4
49	0.004213618414716363
50	3.009727439083117E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	332256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.223931393791197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.00721590574427	20.297808495423315
2	15.960037817856348	9.966702517341192
3	6.4142964082763125	6.008386529743827
4	3.200602902545533	3.997416217914027
5	1.8310202658531554	2.8585825580820123
6	1.2359068071542842	2.315392161342294
7	0.9000134499314357	1.9671370749903934
8	0.6667867744183694	1.6655763598981195
9	0.5192763316294521	1.4592463697895819
>10	3.5631724536562257	22.30446672085542
>50	0.4255873110981219	9.039298185579712
>100	0.2611331979103231	15.272449165369565
>500	0.013953682331085964	2.4856047169395152
>1k	9.966915950775689E-4	0.36193292673101995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1163	0.35003130116536646	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	731	0.22001107579697582	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	701	0.21098189347972646	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	625	0.1881079649426948	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	603	0.18148656457671195	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	573	0.17245738225946258	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	550	0.16553500914957142	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	543	0.16342819994221322	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	541	0.1628262544543966	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	535	0.16102041799094674	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	533	0.1604184725031301	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT	519	0.15620485408841375	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	518	0.15590388134450545	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	513	0.15439901762496389	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	502	0.15108831744197246	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	499	0.15018539921024754	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	497	0.1495834537224309	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	451	0.13573870750264855	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT	450	0.13543773475874027	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	437	0.1315250890879322	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC	426	0.12821438890494077	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA	424	0.12761244341712416	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	424	0.12761244341712416	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	415	0.12490368872194935	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	415	0.12490368872194935	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	390	0.11737937012424154	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	382	0.11497158817297505	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA	375	0.11286477896561688	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	373	0.11226283347780025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	373	0.11226283347780025	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC	368	0.1107579697582587	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	367	0.11045699701435038	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	364	0.10955407878262544	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	360	0.10835018780699221	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	359	0.10804921506308389	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT	357	0.10744726957526726	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	351	0.1056414331118174	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	349	0.10503948762400077	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	347	0.10443754213618414	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	338	0.10172878744100933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	3.009727439083117E-4	0.0
21	0.0	0.0	0.0	9.02918231724935E-4	0.0
22	0.0	0.0	0.0	0.0015048637195415582	0.0
23	0.0	0.0	0.0	0.0024077819512664934	0.0
24	0.0	0.0	0.0	0.003310700182991428	0.0
25	0.0	0.0	0.0	0.004213618414716363	0.0
26	0.0	0.0	0.0	0.004815563902532987	0.0
27	0.0	0.0	0.0	0.005718482134257921	0.0
28	0.0	0.0	0.0	0.006320427622074545	0.0
29	0.0	0.0	0.0	0.009631127805065974	0.0
30	0.0	0.0	0.0	0.013844746219782336	0.0
31	0.0	0.0	0.0	0.02889338341519792	0.0
32	0.0	0.0	0.0	0.04574785707406337	0.0
33	0.0	0.0	0.0	0.05868968506212077	0.0
34	0.0	0.0	0.0	0.06922373109891168	0.0
35	0.0	0.0	0.0	0.08938890494076857	0.0
36	0.0	0.0	0.0	0.10654435134354233	0.0
37	0.0	0.0	0.0	0.13784551671000675	0.0
38	0.0	0.0	0.0	0.17456419146682076	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCAAG	30	1.2992354E-4	36.666035	17
CACTCCG	25	0.0023505432	35.199398	37
TCCGTGC	25	0.0023505432	35.199398	40
GTAAGTA	25	0.0023505432	35.199398	39
CGGAGTA	25	0.0023505432	35.199398	32
AGGGGTC	75	2.1555024E-9	29.33283	5
TTGGACG	30	0.0057395766	29.332829	19
ATAGCCG	30	0.0057395766	29.332829	9
TTTCCGG	30	0.0057395766	29.332829	28
TAGCCGA	30	0.0057395766	29.332829	10
ACACTAG	30	0.0057395766	29.332829	42
CCTATAT	30	0.0057395766	29.332829	4
TACACCT	70	3.57486E-8	28.28523	5
AGGACCT	180	0.0	28.11063	5
GGACATG	175	0.0	27.65667	6
GAGTAGT	40	7.0220337E-4	27.49953	34
TACACGG	105	3.6379788E-12	27.237627	5
TAAGGTC	50	8.324537E-5	26.39955	34
AGGTAAT	110	7.2759576E-12	25.999557	12
GGGTCCT	85	8.190909E-9	25.88191	7
>>END_MODULE