FastQCFastQC Report
Thu 2 Feb 2017
SRR4062059_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062059_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15808
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGAACATTCAAATAAGAACT540.3415991902834008No Hit
ATATAATGCCAGTCTTAAGGGCGGA500.31629554655870445No Hit
CCTTAGACCATGGCCAAGTTCATCC500.31629554655870445No Hit
GTTCAATATAATGCCAGTCTTAAGG480.30364372469635625No Hit
ACCTTAGACCATGGCCAAGTTCATC460.2909919028340081No Hit
GTACATGGGCAGCCGGCGGTTCGGG360.2277327935222672No Hit
TATCAACGCAGAGTACTTTTTTTTT350.22140688259109312No Hit
GTATCAACGCAGAGTACTTTTTTTT310.19610323886639675No Hit
CGTCTAGACTGTGTGCTGTCCTTTC270.1707995951417004No Hit
GCACATACATGCTCCATCGTTTCTC260.1644736842105263No Hit
CTGTTACACATGGTCCAAGAACATT260.1644736842105263No Hit
ATATAAAACCACATCCACACCTCAG240.15182186234817813No Hit
GTACATGGGCGCTCCGGGCCCATTA240.15182186234817813No Hit
CCCTACAGCTATTCAGAGTGTGAAA240.15182186234817813No Hit
GTCCACTGTTGTTCAGCACATACAT230.14549595141700405No Hit
TACATGCTCCATCGTTTCTCACTCT230.14549595141700405No Hit
GTCTTAAGGGCGGAAATTTGGTTCC210.13284412955465585No Hit
GTATCAACGCAGAGTACATGGGCAG210.13284412955465585No Hit
TATCAACGCAGAGTACATGGGCAGC200.12651821862348178No Hit
ACCCAGAGAAGAGCCCCCCCCTTTG200.12651821862348178No Hit
GAGAAGGCACCGTCGATGGTAGCCG190.1201923076923077No Hit
GTACATGGGGCGCTTGGTCTGTCCA190.1201923076923077No Hit
GTTACACATGGTCCAAGAACATTCA190.1201923076923077No Hit
GGTATCAACGCAGAGTACTTTTTTT190.1201923076923077No Hit
ACGCAGAGTACATGGGCAGCCGGCG180.1138663967611336No Hit
GTCTGTGGCTTTGAGAAAAGGTACT180.1138663967611336No Hit
GTTTTAGCACTTTGAATGATTTTTT180.1138663967611336No Hit
CGCCAAGGTTGAGCTGGTTCCCCCA170.10754048582995951No Hit
CTCTGTCACCAGCTAAAAAATGTCC170.10754048582995951No Hit
GTACTGCTCCAGGGAGCGACTACTT160.10121457489878542No Hit
GGTATCAACGCAGAGTACATGGGCA160.10121457489878542No Hit
ACTGTAAGGTGTTTGAAGCTACCAG160.10121457489878542No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)