FastQCFastQC Report
Thu 2 Feb 2017
SRR4062059_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062059_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15808
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGAACATTCAAATAAGAACTCAAATCAGAAGTTAAAGATTGGTCT650.41118421052631576No Hit
GTTCAATATAATGCCAGTCTTAAGGGCGGAAATTTGGTTCCACTGAAAGT540.3415991902834008No Hit
ATATAATGCCAGTCTTAAGGGCGGAAATTTGGTTCCACTGAAAGTCGGTA450.284665991902834No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA410.2593623481781377No Hit
GCTCCAGGGAGCGACTACTTTCCCAGGAAGCAGGTAAGCGGCTGGAGAAG320.20242914979757085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.18977732793522267No Hit
CCTTAGACCATGGCCAAGTTCATCCGTAACTTCGCGGAGAAGGCACCGTC300.18977732793522267No Hit
CCCTACAGCTATTCAGAGTGTGAAAAAAAATCATTCAAAGTGCTAAAACT290.18345141700404857No Hit
ACCTTAGACCATGGCCAAGTTCATCCGTAACTTCGCGGAGAAGGCACCGT290.18345141700404857No Hit
GTCCACTGTTGTTCAGCACATACATGCTCCATCGTTTCTCACTCTGTCAC280.1771255060728745No Hit
GTACATGGGCAGCCGGCGGTTCGGGGAGACGTACCTTCCACCTTAGACCA280.1771255060728745No Hit
GTACATGGGCGCTCCGGGCCCATTAAACTTGGGGGTAGCTAAACTGAAAC280.1771255060728745No Hit
GTTACACATGGTCCAAGAACATTCAAATAAGAACTCAAATCAGAAGTTAA280.1771255060728745No Hit
GAATAGCTGTAGGGATTTCAGCAGGGGTTGGGGGAACCAGCTCAACCTTG250.15814777327935223No Hit
CTGAATAGCTGTAGGGATTTCAGCAGGGGTTGGGGGAACCAGCTCAACCT230.14549595141700405No Hit
GTATCAACGCAGAGTACATGGGCAGCCGGCGGTTCGGGGAGACGTACCTT210.13284412955465585No Hit
CTGTTACACATGGTCCAAGAACATTCAAATAAGAACTCAAATCAGAAGTT210.13284412955465585No Hit
ATTCAGCACAGCTTCCTTAACTGTAAGGTGTTTGAAGCTACCAGTTTTAG200.12651821862348178No Hit
ATATAAAACCACATCCACACCTCAGTGGCCACCAAACCATTCAGCACAGC200.12651821862348178No Hit
CCTTAGCAGAAAGCTCAGCCTGACCACTGTCAGTCTGCTGTGAGTCACCT200.12651821862348178No Hit
ACTGTAAGGTGTTTGAAGCTACCAGTTTTAGCACTTTGAATGATTTTTTT190.1201923076923077No Hit
GTCTTAAGGGCGGAAATTTGGTTCCACTGAAAGTCGGTACTGCTCCAGGG180.1138663967611336No Hit
GTCTGTGGCTTTGAGAAAAGGTACTTTCAAAAGAGGGCTTTCCAGAGCAC180.1138663967611336No Hit
GTACTGCTCCAGGGAGCGACTACTTTCCCAGGAAGCAGGTAAGCGGCTGG170.10754048582995951No Hit
GCTGTAGGGATTTCAGCAGGGGTTGGGGGAACCAGCTCAACCTTGGCGTA170.10754048582995951No Hit
CATACATGCTCCATCGTTTCTCACTCTGTCACCAGCTAAAAAATGTCCGT170.10754048582995951No Hit
TATCAACGCAGAGTACATGGGCAGCCGGCGGTTCGGGGAGACGTACCTTC160.10121457489878542No Hit
TACATGCTCCATCGTTTCTCACTCTGTCACCAGCTAAAAAATGTCCGTGC160.10121457489878542No Hit
ACCCAGAGAAGAGCCCCCCCCTTTGATACAAATCGGTTGGATTTTCATAT160.10121457489878542No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGT250.00228343435.26
CCAGGGA250.00228343435.24
Produced by FastQC (version 0.11.5)