##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15808
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.327745445344128	32.0	32.0	32.0	32.0	32.0
2	31.614372469635626	32.0	32.0	32.0	32.0	32.0
3	31.689334514170042	32.0	32.0	32.0	32.0	32.0
4	31.700531376518217	32.0	32.0	32.0	32.0	32.0
5	31.671368927125506	32.0	32.0	32.0	32.0	32.0
6	35.394294028340084	36.0	36.0	36.0	36.0	36.0
7	35.28542510121458	36.0	36.0	36.0	36.0	36.0
8	35.359881072874494	36.0	36.0	36.0	36.0	36.0
9	35.31756072874494	36.0	36.0	36.0	36.0	36.0
10	35.269040991902834	36.0	36.0	36.0	36.0	36.0
11	35.375632591093115	36.0	36.0	36.0	36.0	36.0
12	35.30269483805668	36.0	36.0	36.0	36.0	36.0
13	35.29035931174089	36.0	36.0	36.0	36.0	36.0
14	35.25480769230769	36.0	36.0	36.0	36.0	36.0
15	35.31667510121458	36.0	36.0	36.0	36.0	36.0
16	35.27093876518219	36.0	36.0	36.0	36.0	36.0
17	35.2866270242915	36.0	36.0	36.0	36.0	36.0
18	35.27834008097166	36.0	36.0	36.0	36.0	36.0
19	35.21134868421053	36.0	36.0	36.0	36.0	36.0
20	35.23728491902834	36.0	36.0	36.0	36.0	36.0
21	35.20578188259109	36.0	36.0	36.0	36.0	36.0
22	35.21451163967611	36.0	36.0	36.0	36.0	36.0
23	35.22343117408907	36.0	36.0	36.0	36.0	36.0
24	35.15352985829959	36.0	36.0	36.0	36.0	36.0
25	35.223367914979754	36.0	36.0	36.0	36.0	36.0
26	35.08324898785425	36.0	36.0	36.0	36.0	36.0
27	35.1363233805668	36.0	36.0	36.0	36.0	36.0
28	35.138600708502025	36.0	36.0	36.0	36.0	36.0
29	35.06901568825911	36.0	36.0	36.0	36.0	36.0
30	35.078757591093115	36.0	36.0	36.0	36.0	36.0
31	35.05889423076923	36.0	36.0	36.0	36.0	36.0
32	35.0695217611336	36.0	36.0	36.0	36.0	36.0
33	35.020242914979754	36.0	36.0	36.0	36.0	36.0
34	35.0059463562753	36.0	36.0	36.0	36.0	36.0
35	35.00727479757085	36.0	36.0	36.0	36.0	36.0
36	34.98431174089069	36.0	36.0	36.0	36.0	36.0
37	34.95483299595141	36.0	36.0	36.0	36.0	36.0
38	34.965017712550605	36.0	36.0	36.0	36.0	36.0
39	34.891194331983804	36.0	36.0	36.0	36.0	36.0
40	34.9175733805668	36.0	36.0	36.0	36.0	36.0
41	34.84824139676113	36.0	36.0	36.0	32.0	36.0
42	34.84830465587044	36.0	36.0	36.0	32.0	36.0
43	34.844572368421055	36.0	36.0	36.0	36.0	36.0
44	34.81547317813765	36.0	36.0	36.0	32.0	36.0
45	34.805794534412954	36.0	36.0	36.0	32.0	36.0
46	34.84242155870445	36.0	36.0	36.0	36.0	36.0
47	34.73690536437247	36.0	36.0	36.0	32.0	36.0
48	34.73722165991903	36.0	36.0	36.0	32.0	36.0
49	34.62310222672065	36.0	36.0	36.0	32.0	36.0
50	34.350455465587046	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	8.0
24	4.0
25	20.0
26	34.0
27	68.0
28	107.0
29	189.0
30	303.0
31	406.0
32	595.0
33	927.0
34	2246.0
35	10901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.333734101120044	19.686135543884074	11.38391444662406	26.59621590837183
2	16.214335421016006	20.041753653444676	37.07218320997027	26.671727715569048
3	18.009868421052634	22.899797570850204	30.41497975708502	28.67535425101215
4	13.499493927125506	16.542257085020243	35.330212550607285	34.62803643724696
5	14.359817813765183	36.31072874493927	33.51467611336032	15.814777327935223
6	32.01543522267207	36.60804655870445	16.978744939271255	14.397773279352228
7	28.143977732793523	31.46508097165992	21.14752024291498	19.24342105263158
8	27.960526315789476	31.86361336032389	20.008856275303643	20.167004048582996
9	24.727985829959515	14.935475708502025	21.00835020242915	39.32818825910931
10	16.535931174089068	26.391700404858298	30.965334008097166	26.107034412955464
11	34.9127024291498	24.430668016194332	21.792763157894736	18.863866396761132
12	22.68471659919028	23.924595141700404	30.07338056680162	23.317307692307693
13	29.946862348178136	20.38841093117409	25.347925101214575	24.3168016194332
14	22.691042510121456	19.439524291497975	25.847672064777328	32.021761133603235
15	24.614119433198383	26.537196356275306	22.982034412955464	25.86664979757085
16	24.354757085020243	28.023785425101217	23.671558704453442	23.9498987854251
17	22.279858299595144	28.34008097165992	25.354251012145752	24.025809716599188
18	23.918269230769234	26.530870445344128	26.290485829959515	23.260374493927124
19	23.741143724696357	25.733805668016196	26.847165991902834	23.677884615384613
20	24.620445344129553	25.581983805668017	26.208248987854248	23.58932186234818
21	24.69003036437247	24.626771255060728	26.834514170040485	23.848684210526315
22	23.443825910931174	25.923582995951417	25.961538461538463	24.671052631578945
23	24.019483805668017	26.391700404858298	25.347925101214575	24.24089068825911
24	23.911943319838056	24.607793522267208	25.841346153846157	25.638917004048583
25	24.70900809716599	24.98102226720648	25.68952429149798	24.620445344129553
26	23.92610868602518	25.748086290883787	25.836654646675523	24.489150376415513
27	24.50657894736842	25.733805668016196	25.87297570850202	23.88663967611336
28	24.362624153855887	26.013791358259	25.52033909027646	24.103245397608656
29	23.848684210526315	25.61361336032389	26.815536437246962	23.722165991902834
30	23.766447368421055	25.733805668016196	25.898279352226723	24.601467611336034
31	24.702682186234817	25.240384615384613	25.664220647773277	24.392712550607285
32	24.671052631578945	25.92990890688259	26.34741902834008	23.051619433198383
33	25.379554655870447	24.791244939271255	25.904605263157894	23.924595141700404
34	23.412196356275302	24.962044534412957	27.39752024291498	24.22823886639676
35	24.253542510121456	25.07591093117409	26.056427125506072	24.614119433198383
36	24.3168016194332	25.77176113360324	25.961538461538463	23.9498987854251
37	25.455465587044536	25.335273279352226	25.809716599190285	23.399544534412957
38	24.07009109311741	25.740131578947366	25.417510121457486	24.772267206477732
39	23.987854251012145	26.42332995951417	25.92990890688259	23.658906882591094
40	24.424342105263158	26.524544534412957	25.411184210526315	23.63992914979757
41	22.653087044534413	26.676366396761132	25.980516194331983	24.69003036437247
42	24.557186234817813	25.588309716599188	26.0501012145749	23.804402834008098
43	23.9498987854251	24.87348178137652	26.78390688259109	24.392712550607285
44	24.141203264376543	24.64098184348706	27.633327007022206	23.58448788511419
45	23.96255060728745	25.581983805668017	27.18243927125506	23.273026315789476
46	24.753289473684212	25.974190283400812	24.69003036437247	24.58248987854251
47	23.911943319838056	26.473937246963565	25.5123987854251	24.101720647773277
48	23.5733265848412	25.724408452486397	26.236872073895988	24.465392888776414
49	23.829706477732792	25.961538461538463	26.651062753036435	23.557692307692307
50	23.48326690706649	25.457075978996645	27.12089580565572	23.938761308281144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	4.5
4	7.0
5	4.5
6	2.0
7	1.5
8	1.0
9	2.5
10	4.0
11	8.0
12	12.0
13	20.0
14	28.0
15	45.0
16	62.0
17	60.5
18	59.0
19	54.5
20	50.0
21	59.5
22	69.0
23	93.0
24	117.0
25	138.0
26	159.0
27	213.5
28	268.0
29	291.0
30	314.0
31	424.5
32	535.0
33	584.0
34	633.0
35	676.5
36	720.0
37	680.5
38	641.0
39	697.0
40	753.0
41	761.5
42	770.0
43	775.5
44	781.0
45	821.5
46	862.0
47	859.5
48	857.0
49	866.5
50	876.0
51	896.0
52	916.0
53	1009.5
54	1103.0
55	1050.0
56	997.0
57	938.0
58	879.0
59	850.5
60	822.0
61	765.5
62	709.0
63	638.5
64	568.0
65	480.0
66	392.0
67	352.0
68	312.0
69	266.5
70	221.0
71	171.0
72	121.0
73	96.5
74	72.0
75	56.0
76	40.0
77	40.0
78	40.0
79	26.5
80	13.0
81	11.5
82	10.0
83	6.5
84	3.0
85	2.0
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031629554655870445
2	0.006325910931174089
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.006325910931174089
27	0.0
28	0.006325910931174089
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.006325910931174089
45	0.0
46	0.0
47	0.0
48	0.012651821862348178
49	0.0
50	0.006325910931174089
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	15808.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.36487854251013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40698230511717	70.95774291497976
2	7.452574525745257	11.829453441295547
3	1.1637175195281366	2.770748987854251
4	0.4463574047505181	1.417004048582996
5	0.3507093894468356	1.3917004048582995
6	0.20723736649131194	0.9868421052631579
7	0.1514426908974972	0.8413461538461539
8	0.15941335883947075	1.0121457489878543
9	0.06376534353578829	0.4554655870445344
>10	0.5818587597640683	7.584767206477733
>50	0.015941335883947073	0.7527834008097166
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCAAGAACATTCAAATAAGAACTCAAATCAGAAGTTAAAGATTGGTCT	65	0.41118421052631576	No Hit
GTTCAATATAATGCCAGTCTTAAGGGCGGAAATTTGGTTCCACTGAAAGT	54	0.3415991902834008	No Hit
ATATAATGCCAGTCTTAAGGGCGGAAATTTGGTTCCACTGAAAGTCGGTA	45	0.284665991902834	No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA	41	0.2593623481781377	No Hit
GCTCCAGGGAGCGACTACTTTCCCAGGAAGCAGGTAAGCGGCTGGAGAAG	32	0.20242914979757085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.18977732793522267	No Hit
CCTTAGACCATGGCCAAGTTCATCCGTAACTTCGCGGAGAAGGCACCGTC	30	0.18977732793522267	No Hit
CCCTACAGCTATTCAGAGTGTGAAAAAAAATCATTCAAAGTGCTAAAACT	29	0.18345141700404857	No Hit
ACCTTAGACCATGGCCAAGTTCATCCGTAACTTCGCGGAGAAGGCACCGT	29	0.18345141700404857	No Hit
GTCCACTGTTGTTCAGCACATACATGCTCCATCGTTTCTCACTCTGTCAC	28	0.1771255060728745	No Hit
GTACATGGGCAGCCGGCGGTTCGGGGAGACGTACCTTCCACCTTAGACCA	28	0.1771255060728745	No Hit
GTACATGGGCGCTCCGGGCCCATTAAACTTGGGGGTAGCTAAACTGAAAC	28	0.1771255060728745	No Hit
GTTACACATGGTCCAAGAACATTCAAATAAGAACTCAAATCAGAAGTTAA	28	0.1771255060728745	No Hit
GAATAGCTGTAGGGATTTCAGCAGGGGTTGGGGGAACCAGCTCAACCTTG	25	0.15814777327935223	No Hit
CTGAATAGCTGTAGGGATTTCAGCAGGGGTTGGGGGAACCAGCTCAACCT	23	0.14549595141700405	No Hit
GTATCAACGCAGAGTACATGGGCAGCCGGCGGTTCGGGGAGACGTACCTT	21	0.13284412955465585	No Hit
CTGTTACACATGGTCCAAGAACATTCAAATAAGAACTCAAATCAGAAGTT	21	0.13284412955465585	No Hit
ATTCAGCACAGCTTCCTTAACTGTAAGGTGTTTGAAGCTACCAGTTTTAG	20	0.12651821862348178	No Hit
ATATAAAACCACATCCACACCTCAGTGGCCACCAAACCATTCAGCACAGC	20	0.12651821862348178	No Hit
CCTTAGCAGAAAGCTCAGCCTGACCACTGTCAGTCTGCTGTGAGTCACCT	20	0.12651821862348178	No Hit
ACTGTAAGGTGTTTGAAGCTACCAGTTTTAGCACTTTGAATGATTTTTTT	19	0.1201923076923077	No Hit
GTCTTAAGGGCGGAAATTTGGTTCCACTGAAAGTCGGTACTGCTCCAGGG	18	0.1138663967611336	No Hit
GTCTGTGGCTTTGAGAAAAGGTACTTTCAAAAGAGGGCTTTCCAGAGCAC	18	0.1138663967611336	No Hit
GTACTGCTCCAGGGAGCGACTACTTTCCCAGGAAGCAGGTAAGCGGCTGG	17	0.10754048582995951	No Hit
GCTGTAGGGATTTCAGCAGGGGTTGGGGGAACCAGCTCAACCTTGGCGTA	17	0.10754048582995951	No Hit
CATACATGCTCCATCGTTTCTCACTCTGTCACCAGCTAAAAAATGTCCGT	17	0.10754048582995951	No Hit
TATCAACGCAGAGTACATGGGCAGCCGGCGGTTCGGGGAGACGTACCTTC	16	0.10121457489878542	No Hit
TACATGCTCCATCGTTTCTCACTCTGTCACCAGCTAAAAAATGTCCGTGC	16	0.10121457489878542	No Hit
ACCCAGAGAAGAGCCCCCCCCTTTGATACAAATCGGTTGGATTTTCATAT	16	0.10121457489878542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.006325910931174089	0.0
6	0.0	0.0	0.0	0.006325910931174089	0.0
7	0.0	0.0	0.0	0.006325910931174089	0.0
8	0.0	0.0	0.0	0.006325910931174089	0.0
9	0.0	0.0	0.0	0.006325910931174089	0.0
10	0.0	0.0	0.0	0.006325910931174089	0.0
11	0.0	0.0	0.0	0.006325910931174089	0.0
12	0.0	0.0	0.0	0.006325910931174089	0.0
13	0.0	0.0	0.0	0.006325910931174089	0.0
14	0.0	0.0	0.0	0.006325910931174089	0.0
15	0.0	0.0	0.0	0.006325910931174089	0.0
16	0.0	0.0	0.0	0.006325910931174089	0.0
17	0.0	0.0	0.0	0.006325910931174089	0.0
18	0.0	0.0	0.0	0.006325910931174089	0.0
19	0.0	0.0	0.0	0.006325910931174089	0.0
20	0.0	0.0	0.0	0.006325910931174089	0.0
21	0.0	0.0	0.0	0.006325910931174089	0.0
22	0.0	0.0	0.0	0.006325910931174089	0.0
23	0.0	0.0	0.0	0.006325910931174089	0.0
24	0.0	0.0	0.0	0.006325910931174089	0.0
25	0.0	0.0	0.0	0.006325910931174089	0.0
26	0.0	0.0	0.0	0.006325910931174089	0.0
27	0.0	0.0	0.0	0.006325910931174089	0.0
28	0.0	0.0	0.0	0.006325910931174089	0.0
29	0.0	0.0	0.0	0.012651821862348178	0.0
30	0.0	0.0	0.0	0.018977732793522266	0.0
31	0.0	0.0	0.0	0.044281376518218625	0.0
32	0.0	0.0	0.0	0.07591093117408906	0.0
33	0.0	0.0	0.0	0.10754048582995951	0.0
34	0.0	0.0	0.0	0.1201923076923077	0.0
35	0.0	0.0	0.0	0.18345141700404857	0.0
36	0.0	0.0	0.0	0.20875506072874495	0.0
37	0.0	0.0	0.0	0.2909919028340081	0.0
38	0.0	0.0	0.0	0.4175101214574899	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTGT	25	0.002283434	35.2	6
CCAGGGA	25	0.002283434	35.2	4
>>END_MODULE