FastQCFastQC Report
Thu 2 Feb 2017
SRR4062058_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062058_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771414
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36760.20751783603381252No Hit
TATCAACGCAGAGTACTTTTTTTTT21000.11854936226088311No Hit
GCGCAAGACGGACCAGAGCGAAAGC20260.11437190854311866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG953.0377123E-1015.0022327
CGAACGA707.263341E-613.57152916
CGCGGTC1102.739398E-912.9568410
GTATCAA20550.012.844051
TAGTACT1155.32782E-912.3910464
AGAACCG1551.8189894E-1212.2591965
CGACTGT550.003064665212.09305110
GTAGGAC4750.011.9990723
TAGGACC6200.011.95136454
CGTCGTA2500.011.78208710
AAGACGG2950.011.594295
CTTAGAC1001.9316813E-611.3991173
CGGTTCT3100.011.34039812
ACCGTCG2800.011.1980958
ACGAACG855.3247193E-511.17623815
CCGTCGT2750.011.0565039
GGTATCA8250.011.0480671
CGCGGTT3300.010.94133110
CTACAGT5650.010.9289754
GGTTCTA3400.010.89775713
Produced by FastQC (version 0.11.5)