##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062058_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1771414
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289898916910445	32.0	32.0	32.0	32.0	32.0
2	30.865575184570066	32.0	32.0	32.0	32.0	32.0
3	30.890980877423345	32.0	32.0	32.0	32.0	32.0
4	30.89721488031595	32.0	32.0	32.0	32.0	32.0
5	30.83031691067136	32.0	32.0	32.0	32.0	32.0
6	34.485053748022764	36.0	36.0	36.0	32.0	36.0
7	34.42135435307613	36.0	36.0	36.0	32.0	36.0
8	34.386271645137725	36.0	36.0	36.0	32.0	36.0
9	34.50014733992167	36.0	36.0	36.0	32.0	36.0
10	34.21898607553062	36.0	36.0	36.0	32.0	36.0
11	34.48625504822701	36.0	36.0	36.0	32.0	36.0
12	34.32225047335067	36.0	36.0	36.0	32.0	36.0
13	34.40396316163246	36.0	36.0	36.0	32.0	36.0
14	34.310313117091766	36.0	36.0	36.0	32.0	36.0
15	34.27145828135038	36.0	36.0	36.0	32.0	36.0
16	34.271131423823	36.0	36.0	36.0	32.0	36.0
17	34.21052278010674	36.0	36.0	36.0	32.0	36.0
18	34.213306996557556	36.0	36.0	36.0	32.0	36.0
19	34.21449700634634	36.0	36.0	36.0	32.0	36.0
20	34.20306771878285	36.0	36.0	36.0	32.0	36.0
21	34.18132237861957	36.0	36.0	36.0	32.0	36.0
22	34.147386212370456	36.0	36.0	36.0	32.0	36.0
23	34.12075946108589	36.0	36.0	36.0	32.0	36.0
24	34.090618003470674	36.0	36.0	36.0	32.0	36.0
25	33.75332135796601	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	30.0
5	136.0
6	355.0
7	78.0
8	304.0
9	230.0
10	140.0
11	49.0
12	79.0
13	69.0
14	286.0
15	401.0
16	662.0
17	816.0
18	1084.0
19	1465.0
20	2182.0
21	3031.0
22	4640.0
23	6886.0
24	9681.0
25	13599.0
26	18801.0
27	24685.0
28	32824.0
29	44038.0
30	57804.0
31	78959.0
32	112657.0
33	162602.0
34	362365.0
35	830475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.573855633554345	16.943087227862787	11.779303881968731	25.703753256614135
2	16.73361898148932	18.88179472849312	37.3680853105718	27.016500979445752
3	19.802314441862055	22.163499019701376	27.552234138102143	30.48195240033442
4	13.00560004110122	15.125079252522388	34.71630278726111	37.153017919115285
5	15.245149278384748	35.94681331067715	32.98394697594729	15.824090434990815
6	35.59370000813427	34.4232689822221	16.088342687743463	13.894688321900164
7	31.48499464548596	29.70543108782816	19.418208738453888	19.391365528231994
8	28.489475751419196	33.20186381913381	18.435081393738855	19.87357903570813
9	26.89508884036993	14.705671235524617	18.42534068615953	39.97389923794592
10	16.509266780512373	26.689356670007424	30.59097453394767	26.210402015532537
11	37.37785840308748	21.364469677226786	21.48341022447767	19.774261695208065
12	24.80267579702698	23.40107052231065	28.234813964386397	23.561439716275974
13	29.583945211859756	19.614366738226778	24.698865325677644	26.102822724235814
14	23.503111529586235	19.53795297467428	24.766939058555305	32.19199643718418
15	25.220622649913743	27.188703306343847	21.80699164087177	25.78368240287064
16	25.800061379062058	26.085479669457364	23.37496672474882	24.73949222673177
17	24.186749473508105	26.21793994996773	24.603986529946454	24.991324046577716
18	24.836658191335268	25.434019290750964	25.617522742955572	24.111799774958193
19	25.687575719219574	25.025710630526373	24.94665750501781	24.340056145236243
20	25.868411060332786	24.444225962038306	24.762513391464722	24.92484958616419
21	26.436149023751472	24.325610172987115	24.492127574818408	24.746113228443004
22	26.102671094002527	24.39020531054265	24.86912385313843	24.637999742316392
23	24.517819955231182	24.622736276414113	25.375173306847188	25.48427046150752
24	24.79042829698271	24.99909594304441	25.21409198779523	24.996383772177648
25	24.862009249293024	24.623120972503553	25.197289058770018	25.3175807194334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	182.0
2	392.0
3	602.0
4	602.0
5	602.0
6	1193.5
7	1785.0
8	1785.0
9	1785.0
10	2175.5
11	2566.0
12	2566.0
13	2566.0
14	3426.0
15	4286.0
16	4286.0
17	4286.0
18	7349.5
19	10413.0
20	10413.0
21	10413.0
22	16590.5
23	22768.0
24	22768.0
25	22768.0
26	33717.5
27	44667.0
28	44667.0
29	44667.0
30	56971.5
31	69276.0
32	69276.0
33	69276.0
34	86541.5
35	103807.0
36	103807.0
37	103807.0
38	121664.0
39	139521.0
40	139521.0
41	139521.0
42	161422.5
43	183324.0
44	183324.0
45	183324.0
46	203568.5
47	223813.0
48	223813.0
49	223813.0
50	233373.5
51	242934.0
52	242934.0
53	242934.0
54	229495.5
55	216057.0
56	216057.0
57	216057.0
58	200634.0
59	185211.0
60	185211.0
61	185211.0
62	162898.5
63	140586.0
64	140586.0
65	140586.0
66	115294.5
67	90003.0
68	90003.0
69	90003.0
70	67893.0
71	45783.0
72	45783.0
73	45783.0
74	34214.0
75	22645.0
76	22645.0
77	22645.0
78	17667.5
79	12690.0
80	12690.0
81	12690.0
82	8760.0
83	4830.0
84	4830.0
85	4830.0
86	3472.5
87	2115.0
88	2115.0
89	2115.0
90	1416.5
91	718.0
92	718.0
93	718.0
94	446.5
95	175.0
96	175.0
97	175.0
98	416.0
99	657.0
100	657.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010725894680746566
2	2.2580830906834877E-4
3	8.467811590063079E-4
4	0.009992017676274435
5	0.03358898597391688
6	0.06356503900274019
7	0.10607345318485685
8	0.1088960570482112
9	0.11950904757442359
10	0.13283173780945617
11	0.13904146630883576
12	0.13384787520026373
13	0.12825911955082211
14	0.11442836062038576
15	0.13153334003231318
16	0.11747677279280845
17	0.12171067858784
18	0.11092833182982634
19	0.09698466874485581
20	0.09201688594535214
21	0.09196043386808504
22	0.10172664323529113
23	0.08083937464646887
24	0.09111365270907873
25	0.08744426768671806
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1771414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.182080178496086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.84195208905477	32.48106503109968
2	16.31834830780944	15.398672364793816
3	6.377525438148308	9.027147496893354
4	2.963811532348553	5.59355173412883
5	1.6354459051893464	3.8581869913118414
6	0.9936143878989461	2.8128476229813226
7	0.6133394426448959	2.0257041531653903
8	0.4210699983347411	1.5893566737751175
9	0.31160188366424446	1.323182255293511
>10	1.3369971275321957	11.068912553114302
>50	0.09772167280404041	3.2237327618381806
>100	0.07861139969547865	7.4197028293800535
>500	0.007200589066387646	2.2742679539445856
>1k	0.002760225808781931	1.9036695782800188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3676	0.20751783603381252	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2100	0.11854936226088311	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2026	0.11437190854311866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.693562318012616E-4
2	0.0	0.0	0.0	5.64520772670872E-5	1.693562318012616E-4
3	0.0	0.0	0.0	5.64520772670872E-5	1.693562318012616E-4
4	0.0	0.0	0.0	5.64520772670872E-5	1.693562318012616E-4
5	0.0	0.0	0.0	5.64520772670872E-5	1.693562318012616E-4
6	0.0	0.0	0.0	5.64520772670872E-5	2.258083090683488E-4
7	0.0	0.0	0.0	5.64520772670872E-5	2.258083090683488E-4
8	0.0	0.0	0.0	5.64520772670872E-5	2.258083090683488E-4
9	0.0	0.0	0.0	5.64520772670872E-5	2.258083090683488E-4
10	0.0	0.0	0.0	5.64520772670872E-5	2.8226038633543597E-4
11	0.0	0.0	0.0	1.129041545341744E-4	2.8226038633543597E-4
12	0.0	0.0	0.0	1.129041545341744E-4	6.774249272050464E-4
13	0.0	0.0	0.0	1.129041545341744E-4	9.596853135404824E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	95	3.0377123E-10	15.002232	7
CGAACGA	70	7.263341E-6	13.571529	16
CGCGGTC	110	2.739398E-9	12.95684	10
GTATCAA	2055	0.0	12.84405	1
TAGTACT	115	5.32782E-9	12.391046	4
AGAACCG	155	1.8189894E-12	12.259196	5
CGACTGT	55	0.0030646652	12.093051	10
GTAGGAC	475	0.0	11.999072	3
TAGGACC	620	0.0	11.9513645	4
CGTCGTA	250	0.0	11.782087	10
AAGACGG	295	0.0	11.59429	5
CTTAGAC	100	1.9316813E-6	11.399117	3
CGGTTCT	310	0.0	11.340398	12
ACCGTCG	280	0.0	11.198095	8
ACGAACG	85	5.3247193E-5	11.176238	15
CCGTCGT	275	0.0	11.056503	9
GGTATCA	825	0.0	11.048067	1
CGCGGTT	330	0.0	10.941331	10
CTACAGT	565	0.0	10.928975	4
GGTTCTA	340	0.0	10.897757	13
>>END_MODULE