FastQCFastQC Report
Thu 2 Feb 2017
SRR4062058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771414
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT20550.11600901878386419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26300.025.0232871
GGTATCA12400.023.4304771
TATACGG602.8736077E-421.999892
GTATACG856.090995E-620.7157171
TAGCGGC3150.019.55490330
TCTAGCG3450.019.12979728
GTAGGAC5950.018.1175543
CTAGCGG3400.018.11704329
CGCGGGA3100.017.74384944
GGCGTTA4900.017.51159742
AACGCAG37350.017.4349846
GCGCAAG7100.017.3603531
TACGAAT3550.017.35349341
CAAGACG6850.017.3429784
ACGGACC7250.017.2959758
CGTCGTA3700.017.24266810
ATCAACG38100.017.2072623
TCAACGC38150.017.127044
CGCAATA3500.016.9713436
TATCAAC38750.016.9186232
Produced by FastQC (version 0.11.5)