FastQCFastQC Report
Thu 2 Feb 2017
SRR4062057_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062057_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10825
Sequences flagged as poor quality0
Sequence length25
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTGTACTGGCCAGAAAATGGGA810.7482678983833718No Hit
GTACAGGAAAGTGGCGTAAACAGTT750.6928406466512702No Hit
GTATCAACGCAGAGTACATGGGGGG710.6558891454965358No Hit
CTCCAGGGTTTTGGAGGCATAGATG500.4618937644341801No Hit
ACTAAAGATGGTCTGATCGTCCCCA480.44341801385681295No Hit
GTCCTGACACGTACTGGAACTCCAC380.3510392609699769No Hit
ATTTAAGGCATCTCCTCTTACAAGC350.3233256351039261No Hit
GAGTAAGGGTCCAAAACCAGTGCTG350.3233256351039261No Hit
AGCATGGACAGAGCATCCTGGGGCT350.3233256351039261No Hit
GTCCAATGTTGAGAAGATAGTTGCC330.3048498845265589No Hit
GTTCTGGTGGCACTGGCAGGGCGAT320.2956120092378753No Hit
GTACTACAACTGTCAAGACAAATAC320.2956120092378753No Hit
GTATCAACGCAGAGTACTTTTTTTT310.28637413394919164No Hit
GTCCATGCTGGTGCACATCTCCCAC300.27713625866050806No Hit
TGTCAGGACACTCCCACTCCCCGTC290.2678983833718245No Hit
CTCTTGGAATGGTTCCATCCACTCT290.2678983833718245No Hit
AGGTCATACAGCTCTGGCATTGTTT290.2678983833718245No Hit
GGTCAGGCTTGTAGCTGTTAACAAG290.2678983833718245No Hit
GAGTAATGGTCGCCATCTGAGGAAA280.2586605080831409No Hit
TCCTGGGGCTATCGAAAAGACATGA260.24018475750577367No Hit
TTTTAGTAGTGTACCACACAACCGT260.24018475750577367No Hit
CTCCCACTCCCCGTCGGACCAGATC260.24018475750577367No Hit
GTACATGGGGGGCCCGGAAGCAAGG260.24018475750577367No Hit
GGGTGGGAGTGAGCATGCTCAACGT250.23094688221709006No Hit
TTCCAAGAGCGAGTGGTAGAGGCCG250.23094688221709006No Hit
GTATAGAGCCAACAGAGAAACATTG250.23094688221709006No Hit
GTTCGTGCACTGGGGGGTGTTCTCG250.23094688221709006No Hit
GTCCTTGAGCTCCTTTGATCAGTTC240.22170900692840648No Hit
GGTCCACACAGAGTAAGGGTCCAAA240.22170900692840648No Hit
GTACATGGGGGGGTTGAGGCCAAAC240.22170900692840648No Hit
GTATCAACGCAGAGTACATGGGGTA230.21247113163972287No Hit
GTGTACCACACAACCGTCTTGTTCT230.21247113163972287No Hit
CCTTTGATCAGTTCACCTTTGTCAG220.20323325635103923No Hit
GGTATCAACGCAGAGTACATGGGGG220.20323325635103923No Hit
ACATTGGACCAACTAAAGATGGTCT220.20323325635103923No Hit
ACCCTGGAGGGTGCAGTCGGAAAAG220.20323325635103923No Hit
CTTACAAGCAGAAGGGATGGAAGAT220.20323325635103923No Hit
GGATGTAGGAGCTGAACTTGAGTGG220.20323325635103923No Hit
GGCCAGAACTGCTCCTGTCATCATG220.20323325635103923No Hit
GTTCACCTTTGTCAGCTTCAGAGTC210.19399538106235567No Hit
TATCAACGCAGAGTACATGGGGGGC210.19399538106235567No Hit
CTCCCACCCCGGGCAGCGCCACGAA210.19399538106235567No Hit
GGTGAGGGTAGGCAACGAACGGGGG210.19399538106235567No Hit
GCTCTGGCATTGTTTTTGCCCTGAC210.19399538106235567No Hit
CTCCAGGAATATCACCTACAGCATG210.19399538106235567No Hit
TCTTTAGTTGGTCCAATGTTGAGAA200.18475750577367206No Hit
GTAGTGTACCACACAACCGTCTTGT200.18475750577367206No Hit
ATTTATTACTGTGTTTCCATTGTCA190.17551963048498845No Hit
GATGTGGAAGGCCCTCACCTACTTC190.17551963048498845No Hit
ATCCTTGACAGGGCTATCGTTGTAG190.17551963048498845No Hit
CATCAGGACCAAGCCCTTCCCCTGG190.17551963048498845No Hit
GTACATGGGGTAGTTCCATGATGGC190.17551963048498845No Hit
GTAGAGGCCGTAGCGTATGTTCCTC190.17551963048498845No Hit
TATCAACGCAGAGTACTTTTTTTTT190.17551963048498845No Hit
ATCTTTAGTTGGTCCAATGTTGAGA190.17551963048498845No Hit
CTTTCAGGCTGCCGGGGCCAAGTAC180.16628175519630484No Hit
CCTTAAAGCGGACTCCAGGAATATC180.16628175519630484No Hit
CACCTACTTCGTGGCGCTGCCCGGG170.15704387990762125No Hit
TTGTAGAGCCAAGCAAGGAAGCTGG170.15704387990762125No Hit
ACTTTAACCACCTTAAAGCGGACTC170.15704387990762125No Hit
TTGTAGTACCCTCCATGATGACAGG170.15704387990762125No Hit
GGCTTGTAGCTGTTAACAAGGTCAT170.15704387990762125No Hit
AGTAGGTAGAGTGGATGGAACCATT160.14780600461893764No Hit
CACCAGAACCATTCACTGCCCCAGG160.14780600461893764No Hit
GCCTACCAGCGCTTCATGACAGAAA160.14780600461893764No Hit
CTACCTACTTGATAAGAAAAATGGC160.14780600461893764No Hit
GTCATGAAGCGCTGGTAGGCCGGCA160.14780600461893764No Hit
GCTGTGGACTCTGGGATGCTGCTGC160.14780600461893764No Hit
ATCAGGAAGTGCGTCAGGGTGCAGC160.14780600461893764No Hit
TATCAACGCAGAGTACATGGGGTAG160.14780600461893764No Hit
ACTTTATTGCAGGGATGGTAAGCAG160.14780600461893764No Hit
CCCCAGGGGAAGGGCTTGGTCCTGA150.13856812933025403No Hit
GAGTGGGAGTGTCCTGACACGTACT150.13856812933025403No Hit
TCATGATGCTTTGTGGTCAGGACGA150.13856812933025403No Hit
AGTGGGAGATGTGCACCAGCATGGA150.13856812933025403No Hit
GCTCTACAACGATAGCCCTGTCAAG150.13856812933025403No Hit
GACCAGATCAGGTCAGGCTTGTAGC150.13856812933025403No Hit
CTCCAGTGGATCCTGGGTCCAGCTC150.13856812933025403No Hit
CCACACAACCGTCTTGTTCTTTTCC150.13856812933025403No Hit
GAACTTGAGTGGTGGGCTGTGGACT150.13856812933025403No Hit
GTCCTGATGCGCAGGTGAGGGTAGG150.13856812933025403No Hit
GTGAATGGTTCTGGTGGCACTGGCA150.13856812933025403No Hit
CCTTTGTATAGAGCCAACAGAGAAA150.13856812933025403No Hit
GGACAGAGCATCCTGGGGCTATCGA150.13856812933025403No Hit
CTACTAAAAAACGCAACTGTTTACG150.13856812933025403No Hit
CTACAAGCCTGACCTGATCTGGTCC140.12933025404157045No Hit
GAGCATCCTGGGGCTATCGAAAAGA140.12933025404157045No Hit
TGCTCAACGTGTTCCTCAAGTCGCG140.12933025404157045No Hit
GATCAGTGGGCCGAACTCTTTCAGG140.12933025404157045No Hit
GTTGAGAAGATAGTTGCCTCCCAAA140.12933025404157045No Hit
GACATGACCATGTCGACCATCGCCA140.12933025404157045No Hit
CATTAAGAACGGGAAGAAGATCACA140.12933025404157045No Hit
CCCCTGGGGAGACGGTAACCATACC140.12933025404157045No Hit
CTCTGGGATGCTGCTGCTGCTGCTG140.12933025404157045No Hit
GTTTTGGAGGCATAGATGGCCTCCC140.12933025404157045No Hit
GATGGTAAGCAGCGCCCCAGGCAGT140.12933025404157045No Hit
GTGCACGAACACCCCGAACTTGGCC140.12933025404157045No Hit
ACGCAGAGTACATGGGGTAGTTCCA140.12933025404157045No Hit
GTTTTTGCCCTGACGAAATGCTGAG140.12933025404157045No Hit
GTGAACTGCGGTGCGAAGTCGGCGT140.12933025404157045No Hit
GGGTACTACAACTGTCAAGACAAAT140.12933025404157045No Hit
GAGATGAGGACGCCCTCCAGTGGAT140.12933025404157045No Hit
GTTTGGGAGGCAACTATCTTCTCAA140.12933025404157045No Hit
CTTCCGCACAGCTGCTCCCAACTCA140.12933025404157045No Hit
GCTCTGTGGCTTGTATTTGTCTTGA130.12009237875288684No Hit
GTATGACCTTGTTAACAGCTACAAG130.12009237875288684No Hit
CTTCATGACAGAAAACTACCCGCCC130.12009237875288684No Hit
TTATTACTGTGTTTCCATTGTCAAA130.12009237875288684No Hit
CCCTTACTCTGTGTGGACCACGAAA130.12009237875288684No Hit
GGATGGAAGATCAGAGTAATGGTCG130.12009237875288684No Hit
GCTTCAGAGTCCACGCAAACTCCAC130.12009237875288684No Hit
ACCTACTTGATAAGAAAAATGGCTT130.12009237875288684No Hit
CCGTTGATCTGCAGCCACTTGCCGA130.12009237875288684No Hit
GTCTGAACCAATTCCTCGATGATTT130.12009237875288684No Hit
AGCCTGACCTGATCTGGTCCGACGG130.12009237875288684No Hit
GTCCTGACCACAAAGCATCATGAAG130.12009237875288684No Hit
GTCTGTCCTTGAGCTCCTTTGATCA130.12009237875288684No Hit
ATCCTGGGGCTATCGAAAAGACATG130.12009237875288684No Hit
GGTTCGATGAGGCCAAGTTCGGGGT130.12009237875288684No Hit
GTTGTAGTACCCTCCATGATGACAG130.12009237875288684No Hit
GCTTGGACTCGCGGCCACTGCCGAG130.12009237875288684No Hit
CAAGTGGGAGATGTGCACCAGCATG130.12009237875288684No Hit
GTACCACACAACCGTCTTGTTCTTT130.12009237875288684No Hit
CCTCTTACAAGCAGAAGGGATGGAA130.12009237875288684No Hit
GGGTATGGTTACCGTCTCCCCAGGG130.12009237875288684No Hit
GTGTGGACCACGAAAACCCTTTGGA130.12009237875288684No Hit
CGCTACGGCCTCTACCACTCGCTCT130.12009237875288684No Hit
GGCCAAGCCCTGTGTCTTGGAACTG130.12009237875288684No Hit
GCTTCAGCTACGCCGACTTCGCACC120.11085450346420324No Hit
GTGCAGCTCATTAAGAACGGGAAGA120.11085450346420324No Hit
GGGCTTGGCCAGTTTGTGAAGCCTT120.11085450346420324No Hit
GGTCAGGACGACGTACTTGGCCCCG120.11085450346420324No Hit
GTATGTTCCTCTTCCGCACAGCTGC120.11085450346420324No Hit
GAACGGGGGTCTCTCGTGCTCTTCG120.11085450346420324No Hit
CTTTACTCATCTTCATAGCCGGTCG120.11085450346420324No Hit
GTCAAGGATGAGGTGATAGTGAATG120.11085450346420324No Hit
GGCCTCTACCACTCGCTCTTGGAAT120.11085450346420324No Hit
TAGATGGCCTCCCCGTTGATCTGCA120.11085450346420324No Hit
GTGGTACACTACTAAAAAACGCAAC120.11085450346420324No Hit
AGACATGACCATGTCGACCATCGCC120.11085450346420324No Hit
ACCCTCACCTGCGCATCAGGACCAA120.11085450346420324No Hit
GGTAAGCAGCGCCCCAGGCAGTCTG120.11085450346420324No Hit
GCCTGGGGCAGTGAATGGTTCTGGT120.11085450346420324No Hit
ACCCTCATGTGAACCCACTTCCGAC120.11085450346420324No Hit
GTCTTGTTCTTTTCCGACTGCACCC120.11085450346420324No Hit
CTTCATAGCCGGTCGGAAGTGGGTT120.11085450346420324No Hit
TTCCTGGAGTCCGCTTTAAGGTGGT110.10161662817551961No Hit
GGCCCATGTCGAGTGGCGCCCACGG110.10161662817551961No Hit
GTTCTGGCCCCACCGGTCATTCACT110.10161662817551961No Hit
CCTCAAGTCGCGGCACGAAGAGCAC110.10161662817551961No Hit
GTATTTGTCTTGACAGTTGTAGTAC110.10161662817551961No Hit
GTCCAGCTCAGGTCTCCTTCTAGTC110.10161662817551961No Hit
CTCCCAAACTTACCGTCTGAACCAA110.10161662817551961No Hit
GCCTTCATGATGCTTTGTGGTCAGG110.10161662817551961No Hit
GCCTGAAAGAGTTCGGCCCACTGAT110.10161662817551961No Hit
GTCTTGGAACTGGAACTCGAAGGAC110.10161662817551961No Hit
AGTTCGGGGTGTTCGTGCACTGGGG110.10161662817551961No Hit
TACTGGAACTCCACCAGCTTCCTTG110.10161662817551961No Hit
GACCTGATCTGGTCCGACGGGGAGT110.10161662817551961No Hit
CTGTATGACCTTGTTAACAGCTACA110.10161662817551961No Hit
GAACTGCTCCTGTCATCATGGAGGG110.10161662817551961No Hit
GTACATGGGGGGCTAGAAAGATGGC110.10161662817551961No Hit
GCTGTCAGCGTCTCGGGTCTCTCGG110.10161662817551961No Hit
AAGTAGGTGAGGGCCTTCCACATCC110.10161662817551961No Hit
GTCCAGGCAGTGGCCCTGGCTCCGC110.10161662817551961No Hit
GGCCCCACGTCCTTCGAGTTCCAGT110.10161662817551961No Hit
GCCCTGTGTCTTGGAACTGGAACTC110.10161662817551961No Hit
GTTTGTGAAGCCTTCATGATGCTTT110.10161662817551961No Hit
CCCTGGCTCCGCGCCGCTTCACTCC110.10161662817551961No Hit
GGGGGGTGTTCTCGGTGCCCGCCTG110.10161662817551961No Hit
ACGCAGAGTACTTTTTTTTTTTTTT110.10161662817551961No Hit
AACCAGTGCTGTGGTCCCTTTGTTG110.10161662817551961No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)