FastQCFastQC Report
Thu 2 Feb 2017
SRR4062057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10825
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTGTACTGGCCAGAAAATGGGATCGTGAACCTCAAATCCCCCAAAAC870.8036951501154734No Hit
GTACAGGAAAGTGGCGTAAACAGTTGCGTTTTTTAGTAGTGTACCACACA590.5450346420323325No Hit
AGCATGGACAGAGCATCCTGGGGCTATCGAAAAGACATGACCATGTCGAC570.5265588914549654No Hit
GTACTACAACTGTCAAGACAAATACAAGCCACAGAGCTTGCCAGACCACA420.38799076212471134No Hit
CTCCAGGGTTTTGGAGGCATAGATGGCCTCCCCGTTGATCTGCAGCCACT400.3695150115473441No Hit
GAGTAAGGGTCCAAAACCAGTGCTGTGGTCCCTTTGTTGCCTGGGGGAGG390.36027713625866054No Hit
TGTCAGGACACTCCCACTCCCCGTCGGACCAGATCAGGTCAGGCTTGTAG390.36027713625866054No Hit
TTCCAAGAGCGAGTGGTAGAGGCCGTAGCGTATGTTCCTCTTCCGCACAG350.3233256351039261No Hit
ATCTTTAGTTGGTCCAATGTTGAGAAGATAGTTGCCTCCCAAACTTACCG330.3048498845265589No Hit
GTTCTGGTGGCACTGGCAGGGCGATCGGATGCCGGCCTACCAGCGCTTCA330.3048498845265589No Hit
GTACATGGGGTAGTTCCATGATGGCGTCGGCGGTGCTGTCAGCGTCTCGG330.3048498845265589No Hit
CTTACAAGCAGAAGGGATGGAAGATCAGAGTAATGGTCGCCATCTGAGGA320.2956120092378753No Hit
GTTCTGGCCCCACCGGTCATTCACTATCACCTCATCCTTGACAGGGCTAT310.28637413394919164No Hit
GTCCATGCTGGTGCACATCTCCCACTTGTGGTCTGGCAAGCTCTGTGGCT310.28637413394919164No Hit
ACTAAAGATGGTCTGATCGTCCCCATCTTCCAAGAAAGGCTTCTTGCTGT280.2586605080831409No Hit
TATCAACGCAGAGTACATGGGGTAGTTCCATGATGGCGTCGGCGGTGCTG270.24942263279445726No Hit
TTTTAGTAGTGTACCACACAACCGTCTTGTTCTTTTCCGACTGCACCCTC260.24018475750577367No Hit
CCTTAAAGCGGACTCCAGGAATATCACCTACAGCATGACCTTTTCGACCA260.24018475750577367No Hit
ACCCTCACCTGCGCATCAGGACCAAGCCCTTCCCCTGGGGAGACGGTAAC260.24018475750577367No Hit
GTTCGTGCACTGGGGGGTGTTCTCGGTGCCCGCCTGGGGCAGTGAATGGT260.24018475750577367No Hit
ATCCTTGACAGGGCTATCGTTGTAGAGCCAAGCAAGGAAGCTGGTGGAGT250.23094688221709006No Hit
GTATCAACGCAGAGTACATGGGGTAGTTCCATGATGGCGTCGGCGGTGCT250.23094688221709006No Hit
GTCATACAGCTCTGGCATTGTTTTTGCCCTGACGAAATGCTGAGTTTTGA240.22170900692840648No Hit
TCTTTAGTTGGTCCAATGTTGAGAAGATAGTTGCCTCCCAAACTTACCGT240.22170900692840648No Hit
GTACCACACAACCGTCTTGTTCTTTTCCGACTGCACCCTCCAGGGTTTTG230.21247113163972287No Hit
ATTTATTACTGTGTTTCCATTGTCAAATTTTATGATCTTGGCCTTTCTTT230.21247113163972287No Hit
CTCTTGGAATGGTTCCATCCACTCTACCTACTTGATAAGAAAAATGGCTT230.21247113163972287No Hit
GAGTAATGGTCGCCATCTGAGGAAAGGAGACCGAGCTTAGCATCCAAAGG230.21247113163972287No Hit
TTGTAGAGCCAAGCAAGGAAGCTGGTGGAGTTCCAGTACGTGTCAGGACA230.21247113163972287No Hit
GTCCTGATGCGCAGGTGAGGGTAGGCAACGAACGGGGGTCTCTCGTGCTC230.21247113163972287No Hit
GTGTACCACACAACCGTCTTGTTCTTTTCCGACTGCACCCTCCAGGGTTT220.20323325635103923No Hit
TCATGATGCTTTGTGGTCAGGACGACGTACTTGGCCCCGGCAGCCTGAAA220.20323325635103923No Hit
CCTCTTACAAGCAGAAGGGATGGAAGATCAGAGTAATGGTCGCCATCTGA220.20323325635103923No Hit
TCCTGGGGCTATCGAAAAGACATGACCATGTCGACCATCGCCAAGGAAAA220.20323325635103923No Hit
GTAGAGGCCGTAGCGTATGTTCCTCTTCCGCACAGCTGCTCCCAACTCAC220.20323325635103923No Hit
GGCCAGAACTGCTCCTGTCATCATGGAGGGTACTACAACTGTCAAGACAA220.20323325635103923No Hit
TATCAACGCAGAGTACATGGGGGGGTTGAGGCCAAACAGCCAAATTCTGC220.20323325635103923No Hit
CCTTTGATCAGTTCACCTTTGTCAGCTTCAGAGTCCACGCAAACTCCACC220.20323325635103923No Hit
ACATTGGACCAACTAAAGATGGTCTGATCGTCCCCATCTTCCAAGAAAGG210.19399538106235567No Hit
GGGTATGGTTACCGTCTCCCCAGGGGAAGGGCTTGGTCCTGATGCGCAGG210.19399538106235567No Hit
GTATAGAGCCAACAGAGAAACATTGGCTACTTTAACCACCTTAAAGCGGA210.19399538106235567No Hit
GGTGAGGGTAGGCAACGAACGGGGGTCTCTCGTGCTCTTCGTGCCGCGAC210.19399538106235567No Hit
ATTTAAGGCATCTCCTCTTACAAGCAGAAGGGATGGAAGATCAGAGTAAT210.19399538106235567No Hit
GTACATGGGGGGGTTGAGGCCAAACAGCCAAATTCTGCCATCAGGAAGTG200.18475750577367206No Hit
CCACACAACCGTCTTGTTCTTTTCCGACTGCACCCTCCAGGGTTTTGGAG200.18475750577367206No Hit
GCTCTGGCATTGTTTTTGCCCTGACGAAATGCTGAGTTTTGAAGCCATTT190.17551963048498845No Hit
GTCCTGACACGTACTGGAACTCCACCAGCTTCCTTGCTTGGCTCTACAAC190.17551963048498845No Hit
GGTCCACACAGAGTAAGGGTCCAAAACCAGTGCTGTGGTCCCTTTGTTGC190.17551963048498845No Hit
CCCCAGGGGAAGGGCTTGGTCCTGATGCGCAGGTGAGGGTAGGCAACGAA190.17551963048498845No Hit
GTGCCCGCCTGGGGCAGTGAATGGTTCTGGTGGCACTGGCAGGGCGATCG190.17551963048498845No Hit
CTACTAAAAAACGCAACTGTTTACGCCACTTTCCTGTACTGGCCAGAAAA190.17551963048498845No Hit
GTGAATGACCGGTGGGGCCAGAACTGCTCCTGTCATCATGGAGGGTACTA190.17551963048498845No Hit
GTCAAGGATGAGGTGATAGTGAATGACCGGTGGGGCCAGAACTGCTCCTG190.17551963048498845No Hit
GTCCAATGTTGAGAAGATAGTTGCCTCCCAAACTTACCGTCTGAACCAAT190.17551963048498845No Hit
GTTCACCTTTGTCAGCTTCAGAGTCCACGCAAACTCCACCGGCAGAACGG190.17551963048498845No Hit
GTATCAACGCAGAGTACATGGGGGGGTTGAGGCCAAACAGCCAAATTCTG180.16628175519630484No Hit
ACGCAGAGTACATGGGGTAGTTCCATGATGGCGTCGGCGGTGCTGTCAGC180.16628175519630484No Hit
CACCTACTTCGTGGCGCTGCCCGGGGTGGGAGTGAGCATGCTCAACGTGT180.16628175519630484No Hit
GGGTGGGAGTGAGCATGCTCAACGTGTTCCTCAAGTCGCGGCACGAAGAG180.16628175519630484No Hit
GGTAAGCAGCGCCCCAGGCAGTCTGTCCTTGAGCTCCTTTGATCAGTTCA180.16628175519630484No Hit
GTGCACGAACACCCCGAACTTGGCCTCATCGAACCAGCTCGGCAGTGGCC180.16628175519630484No Hit
ACCCTCATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGAGAA180.16628175519630484No Hit
GTTTACGCCACTTTCCTGTACTGGCCAGAAAATGGGATCGTGAACCTCAA180.16628175519630484No Hit
CTTCATGACAGAAAACTACCCGCCCGGCTTCAGCTACGCCGACTTCGCAC180.16628175519630484No Hit
GTTGTAGTACCCTCCATGATGACAGGAGCAGTTCTGGCCCCACCGGTCAT180.16628175519630484No Hit
CTCCCACCCCGGGCAGCGCCACGAAGTAGGTGAGGGCCTTCCACATCCGA180.16628175519630484No Hit
ACTTTATTGCAGGGATGGTAAGCAGCGCCCCAGGCAGTCTGTCCTTGAGC180.16628175519630484No Hit
CCCCTGGGGAGACGGTAACCATACCCTCTTCCACAACCCTCATGTGAACC170.15704387990762125No Hit
CCTTTGTATAGAGCCAACAGAGAAACATTGGCTACTTTAACCACCTTAAA170.15704387990762125No Hit
GTCCAAAACCAGTGCTGTGGTCCCTTTGTTGCCTGGGGGAGGCCAGGTTC170.15704387990762125No Hit
GTTTTTGCCCTGACGAAATGCTGAGTTTTGAAGCCATTTTTCTTATCAAG170.15704387990762125No Hit
GTGAATGGTTCTGGTGGCACTGGCAGGGCGATCGGATGCCGGCCTACCAG170.15704387990762125No Hit
CCGTTGATCTGCAGCCACTTGCCGACAGCAAGAAGCCTTTCTTGGAAGAT160.14780600461893764No Hit
GTCTTGGAACTGGAACTCGAAGGACGTGGGGCCCCACCGTGATTTGGTCG160.14780600461893764No Hit
GGAACATACGCTACGGCCTCTACCACTCGCTCTTGGAATGGTTCCATCCA160.14780600461893764No Hit
CTTCTGATCTGGTTTCACGTCTGATTTCGGCATATATTTCATGACAGCCA160.14780600461893764No Hit
GGACGACGTACTTGGCCCCGGCAGCCTGAAAGAGTTCGGCCCACTGATCC160.14780600461893764No Hit
GGTCAGGACGACGTACTTGGCCCCGGCAGCCTGAAAGAGTTCGGCCCACT160.14780600461893764No Hit
GGTAGAGGCCGTAGCGTATGTTCCTCTTCCGCACAGCTGCTCCCAACTCA150.13856812933025403No Hit
GGATGGAACCATTCCAAGAGCGAGTGGTAGAGGCCGTAGCGTATGTTCCT150.13856812933025403No Hit
CATCAGGACCAAGCCCTTCCCCTGGGGAGACGGTAACCATACCCTCTTCC150.13856812933025403No Hit
GGATGTAGGAGCTGAACTTGAGTGGTGGGCTGTGGACTCTGGGATGCTGC150.13856812933025403No Hit
ATGGTAAGCAGCGCCCCAGGCAGTCTGTCCTTGAGCTCCTTTGATCAGTT150.13856812933025403No Hit
GATGTGGAAGGCCCTCACCTACTTCGTGGCGCTGCCCGGGGTGGGAGTGA150.13856812933025403No Hit
GAATGGTTCTGGTGGCACTGGCAGGGCGATCGGATGCCGGCCTACCAGCG150.13856812933025403No Hit
ATCGAAAAGACATGACCATGTCGACCATCGCCAAGGAAAATGAAATCATC140.12933025404157045No Hit
CTTTCAGGCTGCCGGGGCCAAGTACGTCGTCCTGACCACAAAGCATCATG140.12933025404157045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12933025404157045No Hit
AGCCTGACCTGATCTGGTCCGACGGGGAGTGGGAGTGTCCTGACACGTAC140.12933025404157045No Hit
GACATGACCATGTCGACCATCGCCAAGGAAAATGAAATCATCGAGGAATT140.12933025404157045No Hit
CTACCTACTTGATAAGAAAAATGGCTTCAAAACTCAGCATTTCGTCAGGG140.12933025404157045No Hit
GTACATGGGGGGCCCGGAAGCAAGGATGTAGGAGCTGAACTTGAGTGGTG140.12933025404157045No Hit
GCATCATGAAGGCTTCACAAACTGGCCAAGCCCTGTGTCTTGGAACTGGA140.12933025404157045No Hit
ACACAGGGCTTGGCCAGTTTGTGAAGCCTTCATGATGCTTTGTGGTCAGG140.12933025404157045No Hit
CACCAGAACCATTCACTGCCCCAGGCGGGCACCGAGAACACCCCCCAGTG140.12933025404157045No Hit
GACCAGATCAGGTCAGGCTTGTAGCTGTTAACAAGGTCATACAGCTCTGG140.12933025404157045No Hit
CTTCTAGTCCTAGCATTGTTATCTTTGTGGCCGAGGTCGTTTTGGGGGAT140.12933025404157045No Hit
ATCAGGAAGTGCGTCAGGGTGCAGCTCATTAAGAACGGGAAGAAGATCAC140.12933025404157045No Hit
ACCCTGGAGGGTGCAGTCGGAAAAGAACAAGACGGTTGTGTGGTACACTA130.12009237875288684No Hit
GAGCTTGCCAGACCACAAGTGGGAGATGTGCACCAGCATGGACAGAGCAT130.12009237875288684No Hit
GGCCAAGCCCTGTGTCTTGGAACTGGAACTCGAAGGACGTGGGGCCCCAC130.12009237875288684No Hit
GAGTGGGAGTGTCCTGACACGTACTGGAACTCCACCAGCTTCCTTGCTTG130.12009237875288684No Hit
CATGAGGGTTGTGGAAGAGGGTATGGTTACCGTCTCCCCAGGGGAAGGGC130.12009237875288684No Hit
CTCCAGTGGATCCTGGGTCCAGCTCAGGTCTCCTTCTAGTCCTAGCATTG130.12009237875288684No Hit
GAGCATCCTGGGGCTATCGAAAAGACATGACCATGTCGACCATCGCCAAG130.12009237875288684No Hit
TTCCATGATGGCGTCGGCGGTGCTGTCAGCGTCTCGGGTCTCTCGGCCGC130.12009237875288684No Hit
GTAGTGTACCACACAACCGTCTTGTTCTTTTCCGACTGCACCCTCCAGGG130.12009237875288684No Hit
GAACTTGAGTGGTGGGCTGTGGACTCTGGGATGCTGCTGCTGCTGCTGCT130.12009237875288684No Hit
GAACTGCTCCTGTCATCATGGAGGGTACTACAACTGTCAAGACAAATACA130.12009237875288684No Hit
GAGCACGAGAGACCCCCGTTCGTTGCCTACCCTCACCTGCGCATCAGGAC130.12009237875288684No Hit
GCCCCAGGATGCTCTGTCCATGCTGGTGCACATCTCCCACTTGTGGTCTG130.12009237875288684No Hit
CTCCCAAACTTACCGTCTGAACCAATTCCTCGATGATTTCATTTTCCTTG130.12009237875288684No Hit
GTTCCAAGACACAGGGCTTGGCCAGTTTGTGAAGCCTTCATGATGCTTTG130.12009237875288684No Hit
GTTCCATCCACTCTACCTACTTGATAAGAAAAATGGCTTCAAAACTCAGC130.12009237875288684No Hit
GATCAGGTCAGGCTTGTAGCTGTTAACAAGGTCATACAGCTCTGGCATTG130.12009237875288684No Hit
GTTCTTTTCCGACTGCACCCTCCAGGGTTTTGGAGGCATAGATGGCCTCC130.12009237875288684No Hit
GTGAACTGCGGTGCGAAGTCGGCGTAGCTGAAGCCGGGCGGGTAGTTTTC120.11085450346420324No Hit
CATTAAGAACGGGAAGAAGATCACAGCGTTCGTGCCCAATGATGGCTGCC120.11085450346420324No Hit
CTCTTCCACAACCCTCATGTGAACCCACTTCCGACCGGCTATGAAGATGA120.11085450346420324No Hit
TAGATGGCCTCCCCGTTGATCTGCAGCCACTTGCCGACAGCAAGAAGCCT120.11085450346420324No Hit
CCAGTGGATCCTGGGTCCAGCTCAGGTCTCCTTCTAGTCCTAGCATTGTT120.11085450346420324No Hit
GCCTGAAAGAGTTCGGCCCACTGATCCGGGTGGAAGAAGCGCGCTGTGAA120.11085450346420324No Hit
GCCCGGGGTGGGAGTGAGCATGCTCAACGTGTTCCTCAAGTCGCGGCACG120.11085450346420324No Hit
CTACAAGCCTGACCTGATCTGGTCCGACGGGGAGTGGGAGTGTCCTGACA120.11085450346420324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.11085450346420324No Hit
CAAGTAGGTAGAGTGGATGGAACCATTCCAAGAGCGAGTGGTAGAGGCCG110.10161662817551961No Hit
CCCTGGCTCCGCGCCGCTTCACTCCGGACTGGCAGAGCTTGGACTCGCGG110.10161662817551961No Hit
GCTTGGACTCGCGGCCACTGCCGAGCTGGTTCGATGAGGCCAAGTTCGGG110.10161662817551961No Hit
AAGTAGGTGAGGGCCTTCCACATCCGAGCTGAACCCTCCTCGCCGTGGGC110.10161662817551961No Hit
ATACAAGCCACAGAGCTTGCCAGACCACAAGTGGGAGATGTGCACCAGCA110.10161662817551961No Hit
GGTTCGATGAGGCCAAGTTCGGGGTGTTCGTGCACTGGGGGGTGTTCTCG110.10161662817551961No Hit
CTCGTGCTCTTCGTGCCGCGACTTGAGGAACACGTTGAGCATGCTCACTC110.10161662817551961No Hit
GTGCACCAGCATGGACAGAGCATCCTGGGGCTATCGAAAAGACATGACCA110.10161662817551961No Hit
GATCTGCAGCCACTTGCCGACAGCAAGAAGCCTTTCTTGGAAGATGGGGA110.10161662817551961No Hit
GTAGTACCCTCCATGATGACAGGAGCAGTTCTGGCCCCACCGGTCATTCA110.10161662817551961No Hit
GCTCCCAACTCACCGACCAAATCACGGTGGGGCCCCACGTCCTTCGAGTT110.10161662817551961No Hit
CCAAGACACAGGGCTTGGCCAGTTTGTGAAGCCTTCATGATGCTTTGTGG110.10161662817551961No Hit
TTGTAGTACCCTCCATGATGACAGGAGCAGTTCTGGCCCCACCGGTCATT110.10161662817551961No Hit
GCCAAATTCTGCCATCAGGAAGTGCGTCAGGGTGCAGCTCATTAAGAACG110.10161662817551961No Hit
ATCCTGGGGCTATCGAAAAGACATGACCATGTCGACCATCGCCAAGGAAA110.10161662817551961No Hit
GTGGAAGAGGGTATGGTTACCGTCTCCCCAGGGGAAGGGCTTGGTCCTGA110.10161662817551961No Hit
CTCTACCACTCGCTCTTGGAATGGTTCCATCCACTCTACCTACTTGATAA110.10161662817551961No Hit
AACCAGTGCTGTGGTCCCTTTGTTGCCTGGGGGAGGCCAGGTTCTCTTTA110.10161662817551961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA250.002251376435.21
TCAACGC550.00420711420.04
CAACGCA550.00420711420.05
Produced by FastQC (version 0.11.5)