FastQCFastQC Report
Thu 2 Feb 2017
SRR4062056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766938
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11550.1505988750068454No Hit
TATCAACGCAGAGTACTTTTTTTTT8830.11513316591432424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC307.721765E-418.9985643
GGTATCA3900.014.8549411
GTTCTAG456.770713E-414.7737661
AAGAATT1058.913048E-1114.47509716
AGAATTT1101.8553692E-1013.81713717
CGCCGGT1051.9841536E-812.6681917
CGGACAT604.085917E-412.6673635
CGCCACT905.397924E-712.66570917
TTGGACT853.939267E-612.2931884
ACTGTTC1103.7956852E-812.0923638
CTTAGGC550.003067739512.0899943
TCCCGAC550.003072243912.0876262
TAATTTT802.8641289E-511.87410314
AACGCCA802.8641289E-511.87410315
GCTCTTA802.8698772E-511.8717771
AGCACGG658.0115715E-411.692955
CTAATTT658.0159627E-411.69218613
GTCCTAA658.0335484E-411.6891341
CGGTCCA1157.039853E-811.56660910
GAATTTC1157.039853E-811.56660918
Produced by FastQC (version 0.11.5)