##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062056_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	766938
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202733467372852	32.0	32.0	32.0	32.0	32.0
2	30.625312606755696	32.0	32.0	32.0	32.0	32.0
3	30.667409881893974	32.0	32.0	32.0	32.0	32.0
4	30.670837799144127	32.0	32.0	32.0	32.0	32.0
5	30.533974063092455	32.0	32.0	32.0	32.0	32.0
6	34.212598671600574	36.0	36.0	36.0	32.0	36.0
7	34.160788746939126	36.0	36.0	36.0	32.0	36.0
8	34.071460796048704	36.0	36.0	36.0	32.0	36.0
9	34.26900740346677	36.0	36.0	36.0	32.0	36.0
10	33.87055146569866	36.0	36.0	36.0	32.0	36.0
11	34.2591043865345	36.0	36.0	36.0	32.0	36.0
12	34.03695213954713	36.0	36.0	36.0	32.0	36.0
13	34.15282069737058	36.0	36.0	36.0	32.0	36.0
14	34.02166667970553	36.0	36.0	36.0	32.0	36.0
15	33.948053167270366	36.0	36.0	36.0	32.0	36.0
16	33.95702260156623	36.0	36.0	36.0	32.0	36.0
17	33.889075779267685	36.0	36.0	36.0	32.0	36.0
18	33.89834901908629	36.0	36.0	36.0	32.0	36.0
19	33.90879966829131	36.0	36.0	36.0	32.0	36.0
20	33.884806855312945	36.0	36.0	36.0	32.0	36.0
21	33.883607279858346	36.0	36.0	36.0	32.0	36.0
22	33.83630880201529	36.0	36.0	36.0	32.0	36.0
23	33.79455184121793	36.0	36.0	36.0	32.0	36.0
24	33.78183112585372	36.0	36.0	36.0	32.0	36.0
25	33.38418750929019	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	12.0
5	63.0
6	158.0
7	43.0
8	127.0
9	99.0
10	52.0
11	15.0
12	42.0
13	31.0
14	90.0
15	160.0
16	275.0
17	350.0
18	497.0
19	758.0
20	1101.0
21	1735.0
22	2593.0
23	3832.0
24	5457.0
25	7640.0
26	10507.0
27	13181.0
28	17680.0
29	23129.0
30	29432.0
31	40057.0
32	54885.0
33	77092.0
34	159613.0
35	316229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.737551015086126	17.11564288787764	11.583325727250205	25.563480369786028
2	17.174444803738513	18.471684729886196	37.6532070472634	26.70066341911189
3	20.47926022124517	21.28454673947625	27.33658264357205	30.899610395706528
4	13.536830904367964	14.794824582450945	33.17389444693658	38.49445006624451
5	15.690714388198312	35.41080256172799	32.69085786583537	16.20762518423833
6	36.681288228294676	32.89290336722126	15.771715758929503	14.65409264555456
7	32.12089568798418	29.14092531800056	18.626838007348766	20.11134098666649
8	28.700932371418762	33.44946097889209	17.8681679286683	19.981438721020844
9	26.26814857748571	15.544137497095445	18.387469028951568	39.80024489646728
10	17.835926708324305	26.536114454921233	29.365369500447823	26.26258933630664
11	37.29144415051275	21.4902945961544	20.624864531730257	20.593396721602588
12	24.71337978793825	23.46936777084484	27.63082820543955	24.186424235777356
13	30.020954233603803	19.22058084352214	24.35482502235771	26.403639900516353
14	23.415712208277746	19.234022666809388	24.383790176671617	32.96647494824125
15	25.493181180187886	26.518778683762136	21.502425267171088	26.485614868878894
16	26.168983158601044	25.784663504906476	22.68321434306012	25.363138993432365
17	24.53328250573116	26.087223298537314	24.291894912191836	25.087599283539692
18	25.05224656987073	25.073001609494582	24.98502112706392	24.889730693570765
19	25.96275950817597	24.959899975072076	24.350927749478277	24.726412767273683
20	26.023081571154695	24.456599050687842	24.523679948423403	24.996639429734067
21	26.380973386074835	24.086169972580855	24.427049551518884	25.105807089825422
22	26.295442116405432	24.063422204297495	24.45145771091681	25.189677968380263
23	25.0576113536776	24.206555297765227	25.014941109843907	25.72089223871326
24	24.92531489121228	24.543438738634094	24.865540701916036	25.66570566823759
25	25.422381729200655	24.263230016313216	24.584665579119086	25.729722675367046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	53.0
2	139.5
3	226.0
4	226.0
5	226.0
6	428.0
7	630.0
8	630.0
9	630.0
10	829.5
11	1029.0
12	1029.0
13	1029.0
14	1427.5
15	1826.0
16	1826.0
17	1826.0
18	2823.0
19	3820.0
20	3820.0
21	3820.0
22	6004.0
23	8188.0
24	8188.0
25	8188.0
26	12092.5
27	15997.0
28	15997.0
29	15997.0
30	20783.0
31	25569.0
32	25569.0
33	25569.0
34	32212.5
35	38856.0
36	38856.0
37	38856.0
38	47615.0
39	56374.0
40	56374.0
41	56374.0
42	65740.0
43	75106.0
44	75106.0
45	75106.0
46	86007.0
47	96908.0
48	96908.0
49	96908.0
50	102829.0
51	108750.0
52	108750.0
53	108750.0
54	105614.0
55	102478.0
56	102478.0
57	102478.0
58	94794.5
59	87111.0
60	87111.0
61	87111.0
62	76158.5
63	65206.0
64	65206.0
65	65206.0
66	52972.5
67	40739.0
68	40739.0
69	40739.0
70	30763.5
71	20788.0
72	20788.0
73	20788.0
74	15215.5
75	9643.0
76	9643.0
77	9643.0
78	7128.0
79	4613.0
80	4613.0
81	4613.0
82	3156.5
83	1700.0
84	1700.0
85	1700.0
86	1216.5
87	733.0
88	733.0
89	733.0
90	492.5
91	252.0
92	252.0
93	252.0
94	164.0
95	76.0
96	76.0
97	76.0
98	171.5
99	267.0
100	267.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010431090909565049
2	2.607772727391262E-4
3	7.823318182173788E-4
4	0.010691868182304175
5	0.034944154547042916
6	0.06389043182108593
7	0.10730984773215045
8	0.1078314022776287
9	0.1173497727326068
10	0.1319533000059979
11	0.14029817273364992
12	0.13416990682428045
13	0.12817202955128054
14	0.1152635545506938
15	0.13599534773345434
16	0.11852327045993288
17	0.12386920455108497
18	0.11200383864145472
19	0.09505331591341151
20	0.09114165682232461
21	0.08931621591315074
22	0.10052963864093317
23	0.07771162727625962
24	0.094010206822455
25	0.08970738182225943
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	766938.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.21276988129834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26921745972788	47.91075597151622
2	12.854189116347614	15.736810407793525
3	3.9393448856089694	7.2341463589755435
4	1.7020651806814995	4.167524969121085
5	0.9011189623471549	2.7579993838915375
6	0.532259703535879	1.954865444977791
7	0.34941683540553264	1.4972140636831262
8	0.24628653999584044	1.2060705038101251
9	0.18814608755779724	1.036524884556785
>10	0.9289032397923922	10.06817724810448
>50	0.057584569394225064	2.484460403835505
>100	0.030397099211810514	3.4321880200014685
>500	8.562563147455458E-4	0.36191743230587053
>1k	2.1406407868638646E-4	0.15134490742696613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1155	0.1505988750068454	No Hit
TATCAACGCAGAGTACTTTTTTTTT	883	0.11513316591432424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.607772727391262E-4
2	0.0	0.0	0.0	0.0	2.607772727391262E-4
3	0.0	0.0	0.0	0.0	2.607772727391262E-4
4	0.0	0.0	0.0	0.0	2.607772727391262E-4
5	0.0	0.0	0.0	0.0	2.607772727391262E-4
6	0.0	0.0	0.0	0.0	3.911659091086894E-4
7	0.0	0.0	0.0	0.0	3.911659091086894E-4
8	0.0	0.0	0.0	0.0	3.911659091086894E-4
9	0.0	0.0	0.0	0.0	3.911659091086894E-4
10	0.0	0.0	0.0	0.0	3.911659091086894E-4
11	0.0	0.0	0.0	0.0	3.911659091086894E-4
12	0.0	0.0	0.0	0.0	0.0015646636364347575
13	0.0	0.0	0.0	0.0	0.0016950522728043206
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	30	7.721765E-4	18.998564	3
GGTATCA	390	0.0	14.854941	1
GTTCTAG	45	6.770713E-4	14.773766	1
AAGAATT	105	8.913048E-11	14.475097	16
AGAATTT	110	1.8553692E-10	13.817137	17
CGCCGGT	105	1.9841536E-8	12.668191	7
CGGACAT	60	4.085917E-4	12.667363	5
CGCCACT	90	5.397924E-7	12.665709	17
TTGGACT	85	3.939267E-6	12.293188	4
ACTGTTC	110	3.7956852E-8	12.092363	8
CTTAGGC	55	0.0030677395	12.089994	3
TCCCGAC	55	0.0030722439	12.087626	2
TAATTTT	80	2.8641289E-5	11.874103	14
AACGCCA	80	2.8641289E-5	11.874103	15
GCTCTTA	80	2.8698772E-5	11.871777	1
AGCACGG	65	8.0115715E-4	11.69295	5
CTAATTT	65	8.0159627E-4	11.692186	13
GTCCTAA	65	8.0335484E-4	11.689134	1
CGGTCCA	115	7.039853E-8	11.566609	10
GAATTTC	115	7.039853E-8	11.566609	18
>>END_MODULE