FastQCFastQC Report
Thu 2 Feb 2017
SRR4062056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766938
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4150.031.2822991
GTATCAA9000.024.6929871
CGACGGT1105.2514224E-921.9979137
CTATACC703.2157302E-521.9979134
TTTGCGC803.6156653E-621.99791317
GACGGTA1158.767529E-921.0414838
GTGTAGG1601.8189894E-1220.6284241
CCTATAC755.473287E-520.5313853
GTTATAT654.9243856E-420.3110641
ACGACGG1201.430999E-820.1647556
ACGGTAT1201.430999E-820.1647559
GTATTAC550.004477839520.0033191
CTCGATT901.001433E-519.55497625
TACGACG1252.2871973E-819.3581645
TTAGGAC708.1248034E-418.8553543
GTATATA951.5935884E-518.529391
CAACTAG600.007402618.3363781
CTAGAAC1203.1613672E-718.3315963
TTATACC851.4310077E-418.1159293
ATTTGCG851.4310077E-418.11592916
Produced by FastQC (version 0.11.5)