##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062056_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	766938
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263721448148353	32.0	32.0	32.0	32.0	32.0
2	31.42847270574675	32.0	32.0	32.0	32.0	32.0
3	31.562718237980125	32.0	32.0	32.0	32.0	32.0
4	31.64589054134754	32.0	32.0	32.0	32.0	32.0
5	31.609263069504966	32.0	32.0	32.0	32.0	32.0
6	35.253488547966064	36.0	36.0	36.0	36.0	36.0
7	35.26178778467099	36.0	36.0	36.0	36.0	36.0
8	35.209318093509516	36.0	36.0	36.0	36.0	36.0
9	35.304756055900214	36.0	36.0	36.0	36.0	36.0
10	35.18501234780386	36.0	36.0	36.0	36.0	36.0
11	35.30348998224107	36.0	36.0	36.0	36.0	36.0
12	35.22564796632844	36.0	36.0	36.0	36.0	36.0
13	35.26243712008011	36.0	36.0	36.0	36.0	36.0
14	35.22034766826002	36.0	36.0	36.0	36.0	36.0
15	35.203586209054706	36.0	36.0	36.0	36.0	36.0
16	35.21406945541882	36.0	36.0	36.0	36.0	36.0
17	35.194294714827016	36.0	36.0	36.0	36.0	36.0
18	35.182710988371944	36.0	36.0	36.0	36.0	36.0
19	35.1856108316448	36.0	36.0	36.0	36.0	36.0
20	35.180399197849106	36.0	36.0	36.0	36.0	36.0
21	35.177560637235345	36.0	36.0	36.0	36.0	36.0
22	35.156059551098004	36.0	36.0	36.0	36.0	36.0
23	35.135151733256144	36.0	36.0	36.0	36.0	36.0
24	35.119857146209995	36.0	36.0	36.0	36.0	36.0
25	35.09582000109526	36.0	36.0	36.0	36.0	36.0
26	35.05874660011631	36.0	36.0	36.0	36.0	36.0
27	35.04224852595647	36.0	36.0	36.0	36.0	36.0
28	35.02975207904681	36.0	36.0	36.0	36.0	36.0
29	35.00624431179574	36.0	36.0	36.0	36.0	36.0
30	34.9848579676584	36.0	36.0	36.0	36.0	36.0
31	34.99469057472703	36.0	36.0	36.0	36.0	36.0
32	34.96189261713463	36.0	36.0	36.0	36.0	36.0
33	34.942692890429214	36.0	36.0	36.0	36.0	36.0
34	34.93674325695167	36.0	36.0	36.0	36.0	36.0
35	34.91188596731418	36.0	36.0	36.0	36.0	36.0
36	34.891065509858684	36.0	36.0	36.0	32.0	36.0
37	34.883114410812865	36.0	36.0	36.0	32.0	36.0
38	34.866118252062094	36.0	36.0	36.0	32.0	36.0
39	34.85266996810694	36.0	36.0	36.0	32.0	36.0
40	34.839818864106356	36.0	36.0	36.0	32.0	36.0
41	34.810417530491385	36.0	36.0	36.0	32.0	36.0
42	34.79373169669517	36.0	36.0	36.0	32.0	36.0
43	34.7762074639671	36.0	36.0	36.0	32.0	36.0
44	34.742937499511044	36.0	36.0	36.0	32.0	36.0
45	34.72952963603316	36.0	36.0	36.0	32.0	36.0
46	34.708315404895835	36.0	36.0	36.0	32.0	36.0
47	34.6754431257807	36.0	36.0	36.0	32.0	36.0
48	34.65950702664362	36.0	36.0	36.0	32.0	36.0
49	34.62944201486952	36.0	36.0	36.0	32.0	36.0
50	34.218277096714466	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	5.0
21	19.0
22	48.0
23	136.0
24	344.0
25	883.0
26	1788.0
27	3386.0
28	6000.0
29	9260.0
30	13871.0
31	21036.0
32	32025.0
33	54733.0
34	125105.0
35	498297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.27098889661316	17.662510776663193	12.023821479764655	26.042678846958996
2	16.55750551890772	18.827005550854143	37.402612304719334	27.212876625518806
3	19.7759398751136	21.641934083242823	27.602397589371748	30.979728452271832
4	13.404473373336568	15.175802998417081	33.20477013787295	38.214953490373404
5	15.303714250695624	35.73326657435151	32.66209263330283	16.300926541650043
6	36.807064987084985	32.902181013564345	15.640919658329173	14.649834341021492
7	32.17456952191702	29.020989962682776	18.7523632940342	20.052077221366005
8	28.733222242215145	33.48914775379496	17.752543230352387	20.025086773637504
9	26.366538103471203	15.204618887054755	18.371758864471445	40.0570841450026
10	17.903142105359233	26.373187923926057	29.525854762705723	26.19781520800899
11	37.4944258857952	21.367046619153047	20.64430240775656	20.49422508729519
12	24.74215647157919	23.39002631242682	27.70028346489547	24.167533751098524
13	30.228519124101293	19.03178614177417	24.37785062156263	26.3618441125619
14	23.573613512435166	19.073510505412433	24.42805024656491	32.92482573558749
15	25.575470246617066	26.481801658021897	21.415160025973417	26.52756806938762
16	26.206200541637926	25.56650972118816	22.82872167451394	25.398568062659972
17	24.68413352839473	25.921391298905515	24.38971598747226	25.00475918522749
18	25.12797644659673	25.070475057957747	25.006975792045772	24.79457270339975
19	26.031960862546903	24.687132467031233	24.524277060205648	24.756629610216212
20	26.14399878999188	24.223632449601467	24.666172058461125	24.96619670194553
21	26.580245078480917	23.925010887451137	24.442262607929194	25.05248142613875
22	26.31894625119632	23.822003864719182	24.747763182943082	25.111286701141424
23	25.10594076705027	24.157363437461697	25.081166926140053	25.655528869347975
24	25.064771315206492	24.445548414520772	24.89461214793025	25.595068122342486
25	25.587596389799437	24.0374058920017	24.74189569430645	25.633102023892413
26	25.01228924114425	24.93757587058027	24.97395463216378	25.076180256111698
27	25.411637998477055	24.456799524341015	24.80076564406939	25.33079683311254
28	25.310591401494804	24.288977786586415	24.991394192905688	25.409036619013097
29	25.124456602523814	24.624283184732974	25.042050554545764	25.209209658197445
30	25.33415739918558	24.600582322483568	24.860580314475005	25.204679963855845
31	25.483060449869804	24.317639220114405	24.699028467936575	25.500271862079217
32	25.481201772251588	24.473683524266757	24.511235647395996	25.533879056085663
33	25.22664893374276	24.300103659371395	24.733256403736952	25.739991003148898
34	25.550832540127523	24.50471359837273	24.72702854237023	25.217425319129518
35	25.55398944111446	24.248797489233144	25.20754638255815	24.989666687094246
36	25.104180792139108	24.698279908623405	24.676244171612755	25.521295127624732
37	25.776903287552987	24.244285280269338	24.710955337106853	25.267856095070822
38	25.110634757959577	24.322696228378305	24.913356751132426	25.6533122625297
39	25.60973224588785	24.314837632915438	24.612385665017243	25.463044456179468
40	25.76991330448264	24.290782684888068	24.726542075815875	25.21276193481342
41	25.031521822750214	24.777521778417558	25.046516691897164	25.144439706935064
42	25.80780605352514	24.55417451218685	24.6298044574725	25.008214976815513
43	25.612368773462997	24.086613752267247	24.667003086455974	25.63401438781378
44	25.019363520431448	24.66025823243005	24.71684926183913	25.603528985299373
45	25.263065934037275	24.510312706182408	24.92926232009159	25.29735903968873
46	25.398087926187486	24.537107062572694	24.494729540132166	25.570075471107657
47	25.386019584577475	24.58594656617944	24.8625037487124	25.165530100530688
48	25.670036954066905	25.001499547527832	24.197742072609397	25.130721425795873
49	25.33394358557092	24.567998789927188	24.44972981994951	25.64832780455238
50	25.324251128864645	24.899565926607824	24.60357716114176	25.17260578338577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	18.5
2	21.0
3	64.0
4	107.0
5	129.5
6	152.0
7	111.0
8	70.0
9	89.5
10	109.0
11	239.5
12	370.0
13	711.0
14	1052.0
15	1243.5
16	1435.0
17	1410.0
18	1385.0
19	1259.0
20	1133.0
21	1298.5
22	1464.0
23	1930.0
24	2396.0
25	2954.5
26	3513.0
27	4366.5
28	5220.0
29	6195.5
30	7171.0
31	8573.5
32	9976.0
33	11599.5
34	13223.0
35	15227.5
36	17232.0
37	19749.0
38	22266.0
39	25090.0
40	27914.0
41	30892.5
42	33871.0
43	36666.0
44	39461.0
45	43529.5
46	47598.0
47	51125.0
48	54652.0
49	57787.5
50	60923.0
51	61824.5
52	62726.0
53	63550.0
54	64374.0
55	63428.5
56	62483.0
57	60019.5
58	57556.0
59	53544.5
60	49533.0
61	43554.0
62	37575.0
63	32584.0
64	27593.0
65	23225.5
66	18858.0
67	15933.0
68	13008.0
69	10744.0
70	8480.0
71	6495.0
72	4510.0
73	3866.5
74	3223.0
75	2389.0
76	1555.0
77	1331.0
78	1107.0
79	921.5
80	736.0
81	581.5
82	427.0
83	330.5
84	234.0
85	178.5
86	123.0
87	90.0
88	57.0
89	38.0
90	19.0
91	14.0
92	9.0
93	9.5
94	10.0
95	7.5
96	5.0
97	5.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030641329546847333
2	0.0037812704547173304
3	1.303886363695631E-4
4	0.0
5	0.0
6	1.303886363695631E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.519431818478155E-4
17	0.0
18	0.0
19	0.0
20	1.303886363695631E-4
21	0.0
22	0.0
23	0.0
24	0.0011734977273260682
25	0.0
26	9.127204545869418E-4
27	2.607772727391262E-4
28	0.0018254409091738835
29	5.215545454782524E-4
30	0.0011734977273260682
31	6.519431818478155E-4
32	5.215545454782524E-4
33	3.911659091086894E-4
34	0.0010431090909565049
35	1.303886363695631E-4
36	2.607772727391262E-4
37	0.001955829545543447
38	0.0
39	3.911659091086894E-4
40	1.303886363695631E-4
41	0.0011734977273260682
42	0.0059978772729999035
43	0.005085156818412962
44	0.0041724363638260195
45	0.002607772727391262
46	0.002868550000130389
47	0.0010431090909565049
48	0.005215545454782524
49	0.005476322727521651
50	0.002216606818282573
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	766938.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.160632871660745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63205822158791	48.25657870183519
2	13.529564144888596	16.820125394480094
3	4.025921152527764	7.507614201975951
4	1.6490636146603157	4.100273517316548
5	0.8797305976408688	2.734230535296037
6	0.5338728344663176	1.9911523958048207
7	0.35365832495829813	1.538853770981749
8	0.25401106588664646	1.263159088953525
9	0.18833394057494407	1.0536261243617042
>10	0.8771713501818872	9.428213139056165
>50	0.048755432786798704	2.038145403457693
>100	0.027015098028926734	2.973892112268938
>500	8.442218106805322E-4	0.29413561421156276
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.607772727391262E-4
2	0.0	0.0	0.0	0.0	2.607772727391262E-4
3	0.0	0.0	0.0	0.0	2.607772727391262E-4
4	0.0	0.0	0.0	0.0	2.607772727391262E-4
5	0.0	0.0	0.0	0.0	2.607772727391262E-4
6	0.0	0.0	0.0	0.0	3.911659091086894E-4
7	0.0	0.0	0.0	0.0	3.911659091086894E-4
8	0.0	0.0	0.0	0.0	3.911659091086894E-4
9	0.0	0.0	0.0	0.0	3.911659091086894E-4
10	0.0	0.0	0.0	0.0	3.911659091086894E-4
11	0.0	0.0	0.0	0.0	3.911659091086894E-4
12	0.0	0.0	0.0	0.0	0.0014342750000651944
13	0.0	0.0	0.0	0.0	0.0016950522728043206
14	0.0	0.0	0.0	1.303886363695631E-4	0.0016950522728043206
15	0.0	0.0	0.0	1.303886363695631E-4	0.001955829545543447
16	0.0	0.0	0.0	2.607772727391262E-4	0.001955829545543447
17	0.0	0.0	0.0	2.607772727391262E-4	0.001955829545543447
18	0.0	0.0	0.0	2.607772727391262E-4	0.0020862181819130098
19	0.0	0.0	0.0	3.911659091086894E-4	0.0020862181819130098
20	0.0	0.0	0.0	5.215545454782524E-4	0.002216606818282573
21	0.0	0.0	0.0	6.519431818478155E-4	0.002346995454652136
22	0.0	0.0	0.0	7.823318182173788E-4	0.0024773840910216993
23	0.0	0.0	0.0	9.127204545869419E-4	0.0024773840910216993
24	0.0	0.0	0.0	0.0014342750000651944	0.0024773840910216993
25	0.0	0.0	0.0	0.0020862181819130098	0.0024773840910216993
26	0.0	0.0	0.0	0.0027381613637608255	0.0024773840910216993
27	0.0	0.0	0.0	0.0036508818183477675	0.002868550000130389
28	0.0	0.0	0.0	0.0045636022729347095	0.002868550000130389
29	0.0	0.0	0.0	0.005737100000260778	0.002868550000130389
30	0.0	0.0	0.0	0.009909536364086797	0.002868550000130389
31	0.0	0.0	0.0	0.013299640909695438	0.002868550000130389
32	0.0	0.0	0.0	0.019036740909956214	0.0029989386364999517
33	0.0	0.0	0.0	0.02307878863741267	0.0029989386364999517
34	0.0	0.0	0.0	0.02803355681945607	0.0033901045456086413
35	0.0	0.0	0.0	0.033379490910608156	0.0033901045456086413
36	0.0	0.0	0.0	0.04081164318367326	0.0033901045456086413
37	0.0	0.0	0.0	0.05189467727508612	0.0033901045456086413
38	0.0	0.0	0.0	0.0657158727302598	0.0037812704547173304
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	415	0.0	31.282299	1
GTATCAA	900	0.0	24.692987	1
CGACGGT	110	5.2514224E-9	21.997913	7
CTATACC	70	3.2157302E-5	21.997913	4
TTTGCGC	80	3.6156653E-6	21.997913	17
GACGGTA	115	8.767529E-9	21.041483	8
GTGTAGG	160	1.8189894E-12	20.628424	1
CCTATAC	75	5.473287E-5	20.531385	3
GTTATAT	65	4.9243856E-4	20.311064	1
ACGACGG	120	1.430999E-8	20.164755	6
ACGGTAT	120	1.430999E-8	20.164755	9
GTATTAC	55	0.0044778395	20.003319	1
CTCGATT	90	1.001433E-5	19.554976	25
TACGACG	125	2.2871973E-8	19.358164	5
TTAGGAC	70	8.1248034E-4	18.855354	3
GTATATA	95	1.5935884E-5	18.52939	1
CAACTAG	60	0.0074026	18.336378	1
CTAGAAC	120	3.1613672E-7	18.331596	3
TTATACC	85	1.4310077E-4	18.115929	3
ATTTGCG	85	1.4310077E-4	18.115929	16
>>END_MODULE