FastQCFastQC Report
Thu 2 Feb 2017
SRR4062055_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062055_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489428
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37180.14935157795284698No Hit
GCGCAAGACGGACCAGAGCGAAAGC25510.10247333925705021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9300.013.1734451
TCCAACG1750.013.02568918
GTATCAA24050.012.636521
GTATAAT2500.012.1563331
CGGTTCT4300.011.71071512
CCGACCA4350.011.5763429
TCGCGTA2500.011.4016069
ACGGTAT3750.011.4016069
GGCGAGG3850.011.34813919
GCGTTAT2100.011.3061131
TGGCGAG8600.011.1545118
GCGTAAC2300.011.15396911
CGCAAGA4600.011.1508282
AGTACGA600.005882620311.0819962
GTCTTAG2400.011.079991
AGGACGT6800.011.0372095
CGCCGGT3200.010.9861427
GTCCTAC10750.010.9548351
TCTTATA2000.010.9236822
GGTCGCG2700.010.90916257
Produced by FastQC (version 0.11.5)