##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062055_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2489428
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228323936261663	32.0	32.0	32.0	32.0	32.0
2	30.72360477989321	32.0	32.0	32.0	32.0	32.0
3	30.75014782512288	32.0	32.0	32.0	32.0	32.0
4	30.765193851760323	32.0	32.0	32.0	32.0	32.0
5	30.674267341734726	32.0	32.0	32.0	32.0	32.0
6	34.33347419567868	36.0	36.0	36.0	32.0	36.0
7	34.267373067226686	36.0	36.0	36.0	32.0	36.0
8	34.21481962924816	36.0	36.0	36.0	32.0	36.0
9	34.36003130036298	36.0	36.0	36.0	32.0	36.0
10	34.019600084838764	36.0	36.0	36.0	32.0	36.0
11	34.3505038105139	36.0	36.0	36.0	32.0	36.0
12	34.15302189900652	36.0	36.0	36.0	32.0	36.0
13	34.251084988198095	36.0	36.0	36.0	32.0	36.0
14	34.14205793459381	36.0	36.0	36.0	32.0	36.0
15	34.09768991109604	36.0	36.0	36.0	32.0	36.0
16	34.10372503241709	36.0	36.0	36.0	32.0	36.0
17	34.03392305380995	36.0	36.0	36.0	32.0	36.0
18	34.04415391808882	36.0	36.0	36.0	32.0	36.0
19	34.04670872184293	36.0	36.0	36.0	32.0	36.0
20	34.02639401501068	36.0	36.0	36.0	32.0	36.0
21	34.0125579048681	36.0	36.0	36.0	32.0	36.0
22	33.97765028753593	36.0	36.0	36.0	32.0	36.0
23	33.94588315066754	36.0	36.0	36.0	32.0	36.0
24	33.92260189891011	36.0	36.0	36.0	32.0	36.0
25	33.5671989710086	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	45.0
5	193.0
6	574.0
7	137.0
8	433.0
9	288.0
10	180.0
11	38.0
12	90.0
13	140.0
14	259.0
15	408.0
16	697.0
17	991.0
18	1480.0
19	2115.0
20	3284.0
21	4976.0
22	7387.0
23	11230.0
24	16212.0
25	22790.0
26	31094.0
27	40218.0
28	52634.0
29	69385.0
30	89936.0
31	120922.0
32	170553.0
33	238370.0
34	513218.0
35	1089150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.4556519594394	17.031217424523312	11.51224124903441	26.000889367002877
2	16.472023481708842	19.280099396367113	38.08197629414319	26.165900827780852
3	19.493800142362936	22.868854171185806	27.997213799885273	29.640131886565985
4	12.745851503823586	15.419811140728479	35.388836773925284	36.445500581522644
5	14.788564016281091	36.47987507489358	33.589012955836196	15.142547952989133
6	35.006761080615235	35.0212318833589	16.454106653031957	13.517900382993913
7	30.692769097878486	30.031334498993857	20.135471843185954	19.140424559941707
8	27.690288344493315	34.08137569554669	18.991455789228592	19.236880170731403
9	26.897381305333667	14.569359575243334	18.42573474586428	40.107524373558725
10	15.88823052173329	27.21328079088796	31.585802277205776	25.312686410172976
11	37.41339250372497	21.17193257407294	22.11624793638432	19.29842698581777
12	24.513269815629187	23.42237798410462	29.101443166108304	22.962909034157892
13	29.534893729469573	19.714638397039074	24.961980879630318	25.78848699386103
14	23.075209227316783	19.917596007287266	25.078120915492676	31.92907384990328
15	24.857276744143952	27.886473017028646	21.982176939429348	25.274073299398054
16	25.42539273595829	26.07889024869423	24.088676825391207	24.407040189956273
17	23.726343096739164	26.45498878867405	25.366917036187946	24.451751078398843
18	24.3680321467322	25.59034471997484	26.3949781991299	23.64664493416306
19	25.484525735755348	25.14451331902944	25.063169288869013	24.3077916563462
20	25.4967246889138	24.92887275581281	25.017731551546945	24.55667100372644
21	25.9946089996896	24.71078859462476	24.619357100696874	24.675245304988767
22	25.599310937183333	24.968916575788867	25.11878452150024	24.312987965527558
23	24.237324590585732	24.94730430832256	25.535148217311686	25.280222883780016
24	24.56845599481488	25.109846102875782	25.67515950200956	24.646538400299786
25	24.71015133726821	24.986852474308048	25.430852860290454	24.87214332813329
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	307.0
1	307.0
2	524.5
3	742.0
4	742.0
5	742.0
6	1551.5
7	2361.0
8	2361.0
9	2361.0
10	2920.5
11	3480.0
12	3480.0
13	3480.0
14	4803.5
15	6127.0
16	6127.0
17	6127.0
18	10381.0
19	14635.0
20	14635.0
21	14635.0
22	24531.0
23	34427.0
24	34427.0
25	34427.0
26	51814.5
27	69202.0
28	69202.0
29	69202.0
30	91475.0
31	113748.0
32	113748.0
33	113748.0
34	137224.0
35	160700.0
36	160700.0
37	160700.0
38	185801.5
39	210903.0
40	210903.0
41	210903.0
42	238892.5
43	266882.0
44	266882.0
45	266882.0
46	293293.5
47	319705.0
48	319705.0
49	319705.0
50	328779.5
51	337854.0
52	337854.0
53	337854.0
54	315776.0
55	293698.0
56	293698.0
57	293698.0
58	269721.5
59	245745.0
60	245745.0
61	245745.0
62	213540.5
63	181336.0
64	181336.0
65	181336.0
66	147801.0
67	114266.0
68	114266.0
69	114266.0
70	85686.5
71	57107.0
72	57107.0
73	57107.0
74	42832.5
75	28558.0
76	28558.0
77	28558.0
78	22368.0
79	16178.0
80	16178.0
81	16178.0
82	11270.0
83	6362.0
84	6362.0
85	6362.0
86	4617.5
87	2873.0
88	2873.0
89	2873.0
90	1914.5
91	956.0
92	956.0
93	956.0
94	606.5
95	257.0
96	257.0
97	257.0
98	638.0
99	1019.0
100	1019.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.828877959113499E-4
2	4.016987034772647E-5
3	6.427179255636235E-4
4	0.010966374604929325
5	0.03603237370191064
6	0.06668198477722594
7	0.10894068838303418
8	0.10906119799407735
9	0.11737636115605674
10	0.1330024407213223
11	0.14059454621704262
12	0.13244006253645416
13	0.12770001783542242
14	0.1164122842677113
15	0.13348447916549505
16	0.11894298609961806
17	0.12464710768899522
18	0.1136807330840659
19	0.0988178810554071
20	0.09383681713228902
21	0.0931940992067254
22	0.10331690653435247
23	0.08166534641692791
24	0.0928727402439436
25	0.09110526594864363
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2489428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.7590150535985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.41180709557334	33.62798555028561
2	15.685362867096492	14.982349625816326
3	5.910191401722421	8.467947603735286
4	2.762767032259676	5.277881291331021
5	1.4800831631619111	3.5343657035013702
6	0.8958128937224648	2.5669884885899297
7	0.5797850540042008	1.938297418542663
8	0.4165213593128663	1.5914119895654797
9	0.2832005091958085	1.2172839643682443
>10	1.4104996907887917	11.773122770219592
>50	0.08457883109997989	2.7990665041818072
>100	0.0666473930820813	6.5009212876972935
>500	0.00852326892036809	2.7606121150924583
>1k	0.004219440059586914	2.961765687073008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3718	0.14935157795284698	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2551	0.10247333925705021	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.205096110431794E-4
2	0.0	0.0	0.0	0.0	1.205096110431794E-4
3	0.0	0.0	0.0	0.0	1.205096110431794E-4
4	0.0	0.0	0.0	0.0	1.205096110431794E-4
5	0.0	0.0	0.0	0.0	1.205096110431794E-4
6	0.0	0.0	0.0	0.0	1.205096110431794E-4
7	0.0	0.0	0.0	0.0	1.205096110431794E-4
8	0.0	0.0	0.0	0.0	1.205096110431794E-4
9	0.0	0.0	0.0	0.0	1.205096110431794E-4
10	0.0	0.0	0.0	0.0	1.205096110431794E-4
11	0.0	0.0	0.0	0.0	1.205096110431794E-4
12	0.0	0.0	0.0	0.0	2.410192220863588E-4
13	0.0	0.0	0.0	0.0	2.811890924340853E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	930	0.0	13.173445	1
TCCAACG	175	0.0	13.025689	18
GTATCAA	2405	0.0	12.63652	1
GTATAAT	250	0.0	12.156333	1
CGGTTCT	430	0.0	11.710715	12
CCGACCA	435	0.0	11.576342	9
TCGCGTA	250	0.0	11.401606	9
ACGGTAT	375	0.0	11.401606	9
GGCGAGG	385	0.0	11.348139	19
GCGTTAT	210	0.0	11.306113	1
TGGCGAG	860	0.0	11.15451	18
GCGTAAC	230	0.0	11.153969	11
CGCAAGA	460	0.0	11.150828	2
AGTACGA	60	0.0058826203	11.081996	2
GTCTTAG	240	0.0	11.07999	1
AGGACGT	680	0.0	11.037209	5
CGCCGGT	320	0.0	10.986142	7
GTCCTAC	1075	0.0	10.954835	1
TCTTATA	200	0.0	10.923682	2
GGTCGCG	270	0.0	10.9091625	7
>>END_MODULE