FastQCFastQC Report
Thu 2 Feb 2017
SRR4062055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489428
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26640.1070125346063433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26800.027.8388791
GGTATCA11150.022.1070121
GTCCTAC11550.021.1508521
TAGGACG9100.019.34024
TCAACGC38750.018.962134
ATCAACG39350.018.6733
AACGCAG39700.018.6192056
CGTCGTA7450.018.3082910
CAACGCA40150.018.3009365
ATTTCGT5050.018.29769742
ATACCGT7500.018.1862346
AACCGCT1701.2732926E-1018.1172187
TCCTACA15100.018.067252
ACCGTCG7400.017.8374168
GTAGGAC13500.017.7625433
TACCGTC7600.017.6574757
CCGTCGT7400.017.5401259
TACGCTC1154.2775246E-617.21698434
TATCAAC43500.017.095222
GTCCTAA6450.017.060711
Produced by FastQC (version 0.11.5)