##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062055_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2489428
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30385494177779	32.0	32.0	32.0	32.0	32.0
2	31.46041178937491	32.0	32.0	32.0	32.0	32.0
3	31.584423008016298	32.0	32.0	32.0	32.0	32.0
4	31.65532885466059	32.0	32.0	32.0	32.0	32.0
5	31.63731025761741	32.0	32.0	32.0	32.0	32.0
6	35.28674619229799	36.0	36.0	36.0	36.0	36.0
7	35.2858576347659	36.0	36.0	36.0	36.0	36.0
8	35.23820371587369	36.0	36.0	36.0	36.0	36.0
9	35.321467823130455	36.0	36.0	36.0	36.0	36.0
10	35.20915848942006	36.0	36.0	36.0	36.0	36.0
11	35.323093096084726	36.0	36.0	36.0	36.0	36.0
12	35.24696878158356	36.0	36.0	36.0	36.0	36.0
13	35.28146465774467	36.0	36.0	36.0	36.0	36.0
14	35.24834700983519	36.0	36.0	36.0	36.0	36.0
15	35.22494444506931	36.0	36.0	36.0	36.0	36.0
16	35.240169629328506	36.0	36.0	36.0	36.0	36.0
17	35.21758211123198	36.0	36.0	36.0	36.0	36.0
18	35.21774801279651	36.0	36.0	36.0	36.0	36.0
19	35.20693990748075	36.0	36.0	36.0	36.0	36.0
20	35.203253518478945	36.0	36.0	36.0	36.0	36.0
21	35.19902885321447	36.0	36.0	36.0	36.0	36.0
22	35.17998552277873	36.0	36.0	36.0	36.0	36.0
23	35.149756891944655	36.0	36.0	36.0	36.0	36.0
24	35.13676796436772	36.0	36.0	36.0	36.0	36.0
25	35.120440117167476	36.0	36.0	36.0	36.0	36.0
26	35.07838507480433	36.0	36.0	36.0	36.0	36.0
27	35.0653266533517	36.0	36.0	36.0	36.0	36.0
28	35.04380765380642	36.0	36.0	36.0	36.0	36.0
29	35.02364358398797	36.0	36.0	36.0	36.0	36.0
30	35.000872489583955	36.0	36.0	36.0	36.0	36.0
31	35.0044938033958	36.0	36.0	36.0	36.0	36.0
32	34.984152182750414	36.0	36.0	36.0	36.0	36.0
33	34.9662565055105	36.0	36.0	36.0	36.0	36.0
34	34.95803373305032	36.0	36.0	36.0	36.0	36.0
35	34.94391603211661	36.0	36.0	36.0	36.0	36.0
36	34.9173677648038	36.0	36.0	36.0	36.0	36.0
37	34.90099573074618	36.0	36.0	36.0	36.0	36.0
38	34.885075607729966	36.0	36.0	36.0	32.0	36.0
39	34.85673576419965	36.0	36.0	36.0	32.0	36.0
40	34.855025732818945	36.0	36.0	36.0	32.0	36.0
41	34.829478096976494	36.0	36.0	36.0	32.0	36.0
42	34.803182899846874	36.0	36.0	36.0	32.0	36.0
43	34.80462700668588	36.0	36.0	36.0	32.0	36.0
44	34.746121599017926	36.0	36.0	36.0	32.0	36.0
45	34.73385171212021	36.0	36.0	36.0	32.0	36.0
46	34.7033294395339	36.0	36.0	36.0	32.0	36.0
47	34.69081491812577	36.0	36.0	36.0	32.0	36.0
48	34.65770530419036	36.0	36.0	36.0	32.0	36.0
49	34.63992411108094	36.0	36.0	36.0	32.0	36.0
50	34.24247618328387	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	22.0
21	67.0
22	135.0
23	398.0
24	1043.0
25	2454.0
26	5366.0
27	10221.0
28	18148.0
29	29245.0
30	44540.0
31	67153.0
32	102568.0
33	175926.0
34	402139.0
35	1630001.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.129585890593596	17.51524712436301	11.853097201472595	26.502069783570796
2	15.872327211534484	19.761330590394653	37.75786857435079	26.608473623720073
3	18.89574411419835	23.22735027353728	28.329817793119126	29.54708781914524
4	12.52392115779207	15.758399118191004	35.55933330869581	36.158346415321105
5	14.424403688077616	36.837191851779544	33.52257366855907	15.215830791583764
6	35.137847148577336	35.2126837372822	16.302767226474877	13.34670188766558
7	30.740435152171504	29.966642939663245	20.212112983384134	19.080808924781113
8	27.80028183181036	34.151620372230084	18.85336711887229	19.194730677087264
9	26.959727294784187	14.310998349821727	18.358032447614473	40.37124190777962
10	15.824203722382702	27.137771061372757	31.666283044250743	25.371742171993795
11	37.55818605719868	21.04451303672972	22.168586518670153	19.228714387401443
12	24.444410523220593	23.423412928592434	29.131712184485753	23.00046436370122
13	29.5921392384114	19.60402148606025	25.060134295910547	25.74370497961781
14	23.148209146840156	19.740157176668696	25.073591202477036	32.03804247401411
15	24.882061260659075	27.815546382542493	22.047353849960714	25.255038506837714
16	25.479651034339806	25.941526801086525	24.128650357071983	24.450171807501686
17	23.84073771163496	26.391926177419066	25.303403030736376	24.46393308020959
18	24.468692782994957	25.531307217005043	26.406970924221085	23.593029075778915
19	25.600238769901175	24.97403015795609	25.142422389739643	24.283308682403092
20	25.67597966437216	24.891420665985382	24.991925843086594	24.440673826555862
21	26.168311020969888	24.560310465018656	24.622131920317404	24.649246593694052
22	25.699277103013223	24.879329709475428	25.098697371444363	24.322695816066982
23	24.311658867683207	24.886690792700488	25.54447268387464	25.257177655741664
24	24.806740890590678	25.02020563958281	25.559692199418016	24.6133612704085
25	24.840173550535287	24.813500660194208	25.5922767521257	24.754049037144814
26	24.256864754386463	25.84169237882413	25.786980817612466	24.114462049176936
27	24.8026650363016	25.210470542961378	25.3540781755765	24.632786245160524
28	24.405244191030466	25.23729775611065	25.479124746975458	24.878333305883427
29	24.411046133748744	25.116794554863116	25.383604334192704	25.088554977195436
30	24.288682305814184	25.458685929185982	25.62575470174669	24.626877063253144
31	25.029143404833427	24.97780602181879	25.14109746309774	24.851953110250044
32	24.89974520036298	25.148720157049446	25.010534637365446	24.94100000522213
33	24.382004147154074	24.97768554738497	25.50531891389875	25.134991391562206
34	24.985347939733536	25.26240142748626	25.57010473561818	24.18214589716202
35	25.14095652802661	25.09592595865704	25.67855163050028	24.08456588281608
36	24.138032152067552	25.61841714134216	25.409051104273285	24.834499602317006
37	25.074857696062086	25.269724711676357	25.155921731829885	24.499495860431672
38	24.586772543732938	25.18956161817092	25.285045399987467	24.938620438108675
39	25.289887965421524	24.852595594940166	24.982425554649495	24.87509088498881
40	25.59135189158291	25.03572112895237	25.290960997789448	24.08196598167527
41	24.586486703623365	25.263195950831523	25.890977745641518	24.25933959990359
42	25.456061029082417	25.599554895844424	25.241021088215387	23.70336298685778
43	24.7852731162062	24.963755234852822	25.611722053167025	24.639249595773954
44	24.321541625825528	25.53335850754423	25.171331930004982	24.97376793662526
45	24.447080534607654	25.652603040841143	25.276484839379492	24.62383158517171
46	24.440029228266898	25.194597350093655	25.137032607835476	25.228340813803968
47	24.858400069414095	25.153610818315038	25.537276332364968	24.450712779905906
48	25.20749617466275	25.593544911608497	24.61327838934522	24.585680524383537
49	24.788518411692728	25.57085366333754	24.738223996542057	24.90240392842767
50	24.833753385389258	25.831514268245755	24.731317994001706	24.603414352363284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	79.0
2	46.0
3	135.0
4	224.0
5	263.0
6	302.0
7	251.0
8	200.0
9	306.0
10	412.0
11	630.5
12	849.0
13	1561.0
14	2273.0
15	3001.0
16	3729.0
17	3728.5
18	3728.0
19	3937.0
20	4146.0
21	5030.5
22	5915.0
23	7387.0
24	8859.0
25	11615.0
26	14371.0
27	19660.0
28	24949.0
29	29805.5
30	34662.0
31	40354.0
32	46046.0
33	53810.0
34	61574.0
35	71338.0
36	81102.0
37	91122.5
38	101143.0
39	107440.5
40	113738.0
41	120961.0
42	128184.0
43	133087.5
44	137991.0
45	149609.0
46	161227.0
47	169984.0
48	178741.0
49	185411.5
50	192082.0
51	189134.0
52	186186.0
53	184737.0
54	183288.0
55	182052.5
56	180817.0
57	173958.0
58	167099.0
59	152453.5
60	137808.0
61	120708.0
62	103608.0
63	88205.0
64	72802.0
65	61441.5
66	50081.0
67	42450.5
68	34820.0
69	30530.0
70	26240.0
71	20269.0
72	14298.0
73	12416.0
74	10534.0
75	7733.0
76	4932.0
77	4395.5
78	3859.0
79	3319.5
80	2780.0
81	2184.0
82	1588.0
83	1306.0
84	1024.0
85	835.0
86	646.0
87	437.0
88	228.0
89	154.0
90	80.0
91	61.0
92	42.0
93	33.0
94	24.0
95	22.0
96	20.0
97	14.5
98	9.0
99	9.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030609441204967565
2	0.005101573534161261
3	2.0084935173863232E-4
4	0.0
5	4.016987034772647E-5
6	1.6067948139090587E-4
7	0.0
8	0.0
9	0.0
10	4.016987034772647E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.623781848681706E-4
17	0.0
18	8.033974069545293E-5
19	8.033974069545293E-5
20	1.6067948139090587E-4
21	4.016987034772647E-5
22	0.0
23	4.016987034772647E-5
24	9.640768883454351E-4
25	3.615288331295382E-4
26	3.615288331295382E-4
27	2.4101922208635877E-4
28	0.0018076441656476908
29	5.623781848681706E-4
30	0.0013657755918226998
31	5.623781848681706E-4
32	8.435672773022557E-4
33	4.0169870347726464E-4
34	6.828877959113499E-4
35	3.2135896278181174E-4
36	3.2135896278181174E-4
37	0.0015264550732136059
38	0.0
39	7.230576662590764E-4
40	2.0084935173863232E-4
41	0.0011247563697363411
42	0.004940894052770355
43	0.004137496645815826
44	0.004338345997554459
45	0.0020486633877340498
46	0.0022896826098204085
47	8.033974069545293E-4
48	0.0042178363865112796
49	0.003454608849904476
50	0.0021691729987772294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2489428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.41631358487153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.84664166067259	33.33300591908405
2	16.62960030290407	16.102878861129565
3	6.27256572652025	9.110835275907661
4	2.9231329707724503	5.661092902527843
5	1.5769185544344058	3.8174291614649247
6	0.9346041667631657	2.715005304943981
7	0.5709292412799847	1.9349602426409165
8	0.4108917995975736	1.591509297501465
9	0.30510681927111344	1.3294932694841661
>10	1.3861896560317284	11.584151224373015
>50	0.07556954598887768	2.5363856519488404
>100	0.05667780893498021	5.470944165625954
>500	0.008087010764096564	2.624187440046737
>1k	0.003084736064652974	2.1881212833207773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2664	0.1070125346063433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.033974069545293E-5
2	0.0	0.0	0.0	0.0	8.033974069545293E-5
3	0.0	0.0	0.0	0.0	8.033974069545293E-5
4	0.0	0.0	0.0	0.0	8.033974069545293E-5
5	0.0	0.0	0.0	0.0	8.033974069545293E-5
6	0.0	0.0	0.0	0.0	1.205096110431794E-4
7	0.0	0.0	0.0	0.0	1.205096110431794E-4
8	0.0	0.0	0.0	0.0	1.205096110431794E-4
9	0.0	0.0	0.0	0.0	1.205096110431794E-4
10	0.0	0.0	0.0	0.0	1.205096110431794E-4
11	0.0	0.0	0.0	0.0	1.205096110431794E-4
12	0.0	0.0	0.0	0.0	2.410192220863588E-4
13	0.0	0.0	0.0	0.0	3.2135896278181174E-4
14	0.0	0.0	0.0	0.0	3.2135896278181174E-4
15	0.0	0.0	0.0	0.0	4.820384441727176E-4
16	0.0	0.0	0.0	0.0	4.820384441727176E-4
17	0.0	0.0	0.0	0.0	4.820384441727176E-4
18	0.0	0.0	0.0	0.0	4.820384441727176E-4
19	0.0	0.0	0.0	0.0	5.22208314520444E-4
20	0.0	0.0	0.0	0.0	6.828877959113499E-4
21	0.0	0.0	0.0	0.0	7.632275366068028E-4
22	0.0	0.0	0.0	0.0	8.033974069545293E-4
23	0.0	0.0	0.0	0.0	8.033974069545293E-4
24	0.0	0.0	0.0	0.0	8.033974069545293E-4
25	0.0	0.0	0.0	4.016987034772647E-5	8.033974069545293E-4
26	0.0	0.0	0.0	4.016987034772647E-5	9.640768883454352E-4
27	0.0	0.0	0.0	4.016987034772647E-5	0.0010042467586931616
28	0.0	0.0	0.0	8.033974069545293E-5	0.0010042467586931616
29	0.0	0.0	0.0	1.205096110431794E-4	0.0010042467586931616
30	0.0	0.0	0.0	4.0169870347726464E-4	0.0010042467586931616
31	0.0	0.0	0.0	0.0015264550732136057	0.001044416629040888
32	0.0	0.0	0.0	0.002691381313297673	0.001044416629040888
33	0.0	0.0	0.0	0.004217836386511279	0.001044416629040888
34	0.0	0.0	0.0	0.00534259275624762	0.0010845864993886147
35	0.0	0.0	0.0	0.007310916403286217	0.0010845864993886147
36	0.0	0.0	0.0	0.009841618235192985	0.0010845864993886147
37	0.0	0.0	0.0	0.013577416177531545	0.0010845864993886147
38	0.0	0.0	0.0	0.019281537766908705	0.0011247563697363411
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2680	0.0	27.838879	1
GGTATCA	1115	0.0	22.107012	1
GTCCTAC	1155	0.0	21.150852	1
TAGGACG	910	0.0	19.3402	4
TCAACGC	3875	0.0	18.96213	4
ATCAACG	3935	0.0	18.673	3
AACGCAG	3970	0.0	18.619205	6
CGTCGTA	745	0.0	18.30829	10
CAACGCA	4015	0.0	18.300936	5
ATTTCGT	505	0.0	18.297697	42
ATACCGT	750	0.0	18.186234	6
AACCGCT	170	1.2732926E-10	18.117218	7
TCCTACA	1510	0.0	18.06725	2
ACCGTCG	740	0.0	17.837416	8
GTAGGAC	1350	0.0	17.762543	3
TACCGTC	760	0.0	17.657475	7
CCGTCGT	740	0.0	17.540125	9
TACGCTC	115	4.2775246E-6	17.216984	34
TATCAAC	4350	0.0	17.09522	2
GTCCTAA	645	0.0	17.06071	1
>>END_MODULE