FastQCFastQC Report
Thu 2 Feb 2017
SRR4062054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822876
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48040.17018104939784817No Hit
TATCAACGCAGAGTACTTTTTTTTT31700.1122968206892545No Hit
GCGCAAGACGGACCAGAGCGAAAGC31450.11141119907498594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.013.3287171
CGTCTTA1450.013.10364315
CGAACGA1701.8189894E-1211.7354716
GTCCTAG2600.011.6860551
ACGGTAT4650.011.6471959
CTAGACA1800.011.6106674
CCTATTC4250.011.3979463
TCGAACG1251.8388164E-811.3979453
GTATCAA29300.011.2124311
GCGTTAT2900.011.1319751
GAGTATA1553.1650416E-1011.0263591
CGGTTCT4900.010.85884612
CGTTATT2750.010.7062112
GTCCTAT4100.010.6528371
AAGACGG6600.010.6522425
CAAGACG6800.010.6172414
CGGTCCA4300.010.60687910
CCGACCA6500.010.5249149
GTTATTC3250.010.521183
GACCATA7150.010.49852311
Produced by FastQC (version 0.11.5)