##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062054_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2822876
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308932096202597	32.0	32.0	32.0	32.0	32.0
2	30.884286451122897	32.0	32.0	32.0	32.0	32.0
3	30.917642149354062	32.0	32.0	32.0	32.0	32.0
4	30.923723181606277	32.0	32.0	32.0	32.0	32.0
5	30.848980968345757	32.0	32.0	32.0	32.0	32.0
6	34.51539848013161	36.0	36.0	36.0	32.0	36.0
7	34.45404048920321	36.0	36.0	36.0	32.0	36.0
8	34.41952072992225	36.0	36.0	36.0	32.0	36.0
9	34.53866021745199	36.0	36.0	36.0	32.0	36.0
10	34.26601203878597	36.0	36.0	36.0	32.0	36.0
11	34.51567054309152	36.0	36.0	36.0	32.0	36.0
12	34.35697458903615	36.0	36.0	36.0	32.0	36.0
13	34.43986027016419	36.0	36.0	36.0	32.0	36.0
14	34.3520884374659	36.0	36.0	36.0	32.0	36.0
15	34.31335347354967	36.0	36.0	36.0	32.0	36.0
16	34.30353653507983	36.0	36.0	36.0	32.0	36.0
17	34.245763540445985	36.0	36.0	36.0	32.0	36.0
18	34.253884690648825	36.0	36.0	36.0	32.0	36.0
19	34.25779913818389	36.0	36.0	36.0	32.0	36.0
20	34.24012000527122	36.0	36.0	36.0	32.0	36.0
21	34.226746410398476	36.0	36.0	36.0	32.0	36.0
22	34.19817200613842	36.0	36.0	36.0	32.0	36.0
23	34.174426365168	36.0	36.0	36.0	32.0	36.0
24	34.14442256762253	36.0	36.0	36.0	32.0	36.0
25	33.80175501864056	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	43.0
5	197.0
6	540.0
7	112.0
8	494.0
9	382.0
10	237.0
11	57.0
12	116.0
13	127.0
14	371.0
15	503.0
16	925.0
17	1129.0
18	1627.0
19	2148.0
20	3258.0
21	4595.0
22	7070.0
23	10620.0
24	14838.0
25	21010.0
26	29251.0
27	38084.0
28	51248.0
29	68411.0
30	89939.0
31	124125.0
32	177871.0
33	256130.0
34	576343.0
35	1341073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51416304147596	17.185423190829095	11.41938615011832	25.881027617576624
2	17.02055000761636	19.201840679875446	37.06986152391006	26.70774778859813
3	19.514356918793208	22.396970443044363	27.99948987815527	30.089182760007155
4	13.150924679373627	15.211294551123078	34.79614539786013	36.841635371643164
5	15.322259150339884	35.89345708485368	33.148948670782666	15.635335094023775
6	35.373027671615695	34.18397693429436	16.414926069365844	14.028069324724102
7	31.237076221524244	29.8119897201992	19.79421958020482	19.15671447807173
8	28.524257273706706	32.92667530553434	18.78645187235754	19.76261554840142
9	26.853037030416587	14.73761565244346	18.67406359957823	39.73528371756172
10	16.802182537430333	26.45989647774612	30.544279317116363	26.193641667707183
11	37.24037624138194	21.296979178306703	21.694505194337577	19.768139385973782
12	24.537600805915247	23.58305946285323	28.160317541958495	23.719022189273026
13	29.352954133282445	19.36260539328441	24.987186631107278	26.297253842325862
14	23.561305890893923	19.46150247745887	24.810550914076103	32.16664071757111
15	25.29513192390791	26.984675948144893	21.843398200104073	25.876793927843124
16	25.99950275347603	25.835078011311825	23.196905012791152	24.968514222420996
17	24.177403485674034	26.113258514321707	24.768516712266965	24.940821287737293
18	24.907261874026513	25.21154222616896	25.48667124387538	24.39452465592914
19	25.65271519806707	25.05254095393168	25.0125789798598	24.282164868141443
20	25.792249401132633	24.503496761365007	24.66893874604478	25.035315091457576
21	26.53556973447907	24.23386832275233	24.46334704934395	24.767214893424647
22	25.928685282274866	24.242252312471297	24.819798335836257	25.00926406941758
23	24.59795731114984	24.445897250084734	25.280011742199164	25.676133696566257
24	24.7098301582332	24.871619678683103	25.14396359859889	25.274586564484807
25	24.88008946134569	24.498020873368514	25.087929667611675	25.533959997674117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	348.0
1	348.0
2	608.5
3	869.0
4	869.0
5	869.0
6	1974.0
7	3079.0
8	3079.0
9	3079.0
10	3732.5
11	4386.0
12	4386.0
13	4386.0
14	6039.5
15	7693.0
16	7693.0
17	7693.0
18	12939.5
19	18186.0
20	18186.0
21	18186.0
22	27518.0
23	36850.0
24	36850.0
25	36850.0
26	53843.0
27	70836.0
28	70836.0
29	70836.0
30	89032.5
31	107229.0
32	107229.0
33	107229.0
34	134478.5
35	161728.0
36	161728.0
37	161728.0
38	191908.5
39	222089.0
40	222089.0
41	222089.0
42	257246.0
43	292403.0
44	292403.0
45	292403.0
46	326463.5
47	360524.0
48	360524.0
49	360524.0
50	373907.5
51	387291.0
52	387291.0
53	387291.0
54	367380.0
55	347469.0
56	347469.0
57	347469.0
58	320839.5
59	294210.0
60	294210.0
61	294210.0
62	257982.5
63	221755.0
64	221755.0
65	221755.0
66	181180.0
67	140605.0
68	140605.0
69	140605.0
70	106535.5
71	72466.0
72	72466.0
73	72466.0
74	54570.5
75	36675.0
76	36675.0
77	36675.0
78	29089.5
79	21504.0
80	21504.0
81	21504.0
82	14874.0
83	8244.0
84	8244.0
85	8244.0
86	6004.0
87	3764.0
88	3764.0
89	3764.0
90	2527.5
91	1291.0
92	1291.0
93	1291.0
94	793.5
95	296.0
96	296.0
97	296.0
98	691.0
99	1086.0
100	1086.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012752951245467389
2	2.125491874244565E-4
3	7.439221559855976E-4
4	0.009777262621524998
5	0.03106760622854139
6	0.06085991733253604
7	0.10545982182710116
8	0.10847093531561429
9	0.11792937415600259
10	0.13362258916084163
11	0.13960939127329716
12	0.13181592106773374
13	0.12593539354899047
14	0.11523708444862615
15	0.1315325221511678
16	0.11863787144741746
17	0.12221578276906248
18	0.11010047908586845
19	0.0954700100181517
20	0.09040425438453549
21	0.09107732681137959
22	0.10064204024548012
23	0.08076869122129346
24	0.09015628033254029
25	0.0864366695526123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2822876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.9973983801976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6864559728081	37.341284928775764
2	14.407032734419847	14.406283101986734
3	4.683045716363427	7.024203069411004
4	2.027422707875155	4.054634432427724
5	1.0530338836040956	2.63244772932003
6	0.6630003089017485	1.9888974342212877
7	0.4243878784564361	1.4852802878839322
8	0.3112636670457027	1.2449898850052146
9	0.22787955314327152	1.0254046321085002
>10	1.3384210619650383	12.034804797949091
>50	0.09486327225391471	3.2859694496438987
>100	0.07029982554219193	7.130040164445553
>500	0.008975528288815704	3.1112477278272137
>1k	0.003917889332398642	3.2345123589942544
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4804	0.17018104939784817	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3170	0.1122968206892545	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3145	0.11141119907498594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.542486457074275E-5
5	0.0	0.0	0.0	0.0	3.542486457074275E-5
6	0.0	0.0	0.0	0.0	1.0627459371222824E-4
7	0.0	0.0	0.0	3.542486457074275E-5	1.0627459371222824E-4
8	0.0	0.0	0.0	3.542486457074275E-5	1.0627459371222824E-4
9	0.0	0.0	0.0	3.542486457074275E-5	1.41699458282971E-4
10	0.0	0.0	0.0	3.542486457074275E-5	1.41699458282971E-4
11	0.0	0.0	0.0	3.542486457074275E-5	1.41699458282971E-4
12	0.0	0.0	0.0	3.542486457074275E-5	3.896735102781702E-4
13	0.0	0.0	0.0	3.542486457074275E-5	4.605232394196557E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1325	0.0	13.328717	1
CGTCTTA	145	0.0	13.103643	15
CGAACGA	170	1.8189894E-12	11.73547	16
GTCCTAG	260	0.0	11.686055	1
ACGGTAT	465	0.0	11.647195	9
CTAGACA	180	0.0	11.610667	4
CCTATTC	425	0.0	11.397946	3
TCGAACG	125	1.8388164E-8	11.397945	3
GTATCAA	2930	0.0	11.212431	1
GCGTTAT	290	0.0	11.131975	1
GAGTATA	155	3.1650416E-10	11.026359	1
CGGTTCT	490	0.0	10.858846	12
CGTTATT	275	0.0	10.706211	2
GTCCTAT	410	0.0	10.652837	1
AAGACGG	660	0.0	10.652242	5
CAAGACG	680	0.0	10.617241	4
CGGTCCA	430	0.0	10.606879	10
CCGACCA	650	0.0	10.524914	9
GTTATTC	325	0.0	10.52118	3
GACCATA	715	0.0	10.498523	11
>>END_MODULE