FastQCFastQC Report
Thu 2 Feb 2017
SRR4062054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822876
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30340.10747903910763348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34800.025.42451
GGTATCA17200.025.208291
CGGTACG901.00292455E-519.55513225
TGCGTTA708.13012E-418.85606412
GTATTAG5750.017.6074141
TCAACGC51600.016.8827554
CGCAATA6000.016.50022536
ATCAACG53000.016.4367963
CAACGCA53200.016.4163555
GTCCTAT5550.016.2590981
AACGCAG53850.016.2590526
GGCGTTA7850.016.2567342
TATCAAC54950.016.0548152
CGCGAAC1004.9487513E-415.39911914
TAGATCG1004.9487513E-415.3991195
ACTCGTA1158.2277504E-515.303472511
GCGTTAT8350.015.28343
ATACGAA6350.015.24592440
CTAGCGG6400.015.12493929
CAATACG6700.015.10602238
Produced by FastQC (version 0.11.5)