##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062054_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2822876
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2926494114513	32.0	32.0	32.0	32.0	32.0
2	31.46653235919679	32.0	32.0	32.0	32.0	32.0
3	31.584581469395044	32.0	32.0	32.0	32.0	32.0
4	31.664525469769128	32.0	32.0	32.0	32.0	32.0
5	31.634366511316827	32.0	32.0	32.0	32.0	32.0
6	35.29335401200761	36.0	36.0	36.0	36.0	36.0
7	35.29606330564998	36.0	36.0	36.0	36.0	36.0
8	35.24766939815989	36.0	36.0	36.0	36.0	36.0
9	35.33395728328131	36.0	36.0	36.0	36.0	36.0
10	35.21383829824619	36.0	36.0	36.0	36.0	36.0
11	35.32769133323603	36.0	36.0	36.0	36.0	36.0
12	35.25375184740669	36.0	36.0	36.0	36.0	36.0
13	35.288207487682776	36.0	36.0	36.0	36.0	36.0
14	35.25346809424148	36.0	36.0	36.0	36.0	36.0
15	35.22998176327972	36.0	36.0	36.0	36.0	36.0
16	35.2403732930529	36.0	36.0	36.0	36.0	36.0
17	35.22708046687138	36.0	36.0	36.0	36.0	36.0
18	35.22211779759366	36.0	36.0	36.0	36.0	36.0
19	35.214753676746696	36.0	36.0	36.0	36.0	36.0
20	35.21730851797953	36.0	36.0	36.0	36.0	36.0
21	35.20702007456225	36.0	36.0	36.0	36.0	36.0
22	35.1912538843364	36.0	36.0	36.0	36.0	36.0
23	35.16209992929197	36.0	36.0	36.0	36.0	36.0
24	35.1440240378961	36.0	36.0	36.0	36.0	36.0
25	35.12692764400562	36.0	36.0	36.0	36.0	36.0
26	35.09140925779241	36.0	36.0	36.0	36.0	36.0
27	35.079399165956985	36.0	36.0	36.0	36.0	36.0
28	35.06035475876376	36.0	36.0	36.0	36.0	36.0
29	35.04185872847408	36.0	36.0	36.0	36.0	36.0
30	35.02029242517206	36.0	36.0	36.0	36.0	36.0
31	35.02561430257652	36.0	36.0	36.0	36.0	36.0
32	35.003860955989566	36.0	36.0	36.0	36.0	36.0
33	34.981999563565665	36.0	36.0	36.0	36.0	36.0
34	34.971211275309294	36.0	36.0	36.0	36.0	36.0
35	34.958780336082775	36.0	36.0	36.0	36.0	36.0
36	34.938470198478434	36.0	36.0	36.0	36.0	36.0
37	34.92421487872652	36.0	36.0	36.0	36.0	36.0
38	34.91372876456494	36.0	36.0	36.0	36.0	36.0
39	34.89928852701996	36.0	36.0	36.0	36.0	36.0
40	34.877701677296486	36.0	36.0	36.0	32.0	36.0
41	34.849074489988226	36.0	36.0	36.0	32.0	36.0
42	34.82769593846843	36.0	36.0	36.0	32.0	36.0
43	34.816767721996996	36.0	36.0	36.0	32.0	36.0
44	34.78290013447278	36.0	36.0	36.0	32.0	36.0
45	34.76164450723305	36.0	36.0	36.0	32.0	36.0
46	34.74284276036213	36.0	36.0	36.0	32.0	36.0
47	34.722571590108814	36.0	36.0	36.0	32.0	36.0
48	34.69865732678304	36.0	36.0	36.0	32.0	36.0
49	34.683000953637354	36.0	36.0	36.0	32.0	36.0
50	34.268370626269096	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	26.0
21	64.0
22	171.0
23	499.0
24	1303.0
25	2995.0
26	6323.0
27	11939.0
28	20344.0
29	32890.0
30	49739.0
31	73268.0
32	112571.0
33	192531.0
34	448423.0
35	1869785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.107123430291416	17.74551629534423	11.81424416740109	26.333116106963256
2	16.45378091124196	19.67705605836075	36.733801699708685	27.1353613306886
3	18.904036603907798	22.845878730425937	28.240569520076747	30.00951514558952
4	12.916259871138514	15.580138837129226	34.855268173309774	36.648333118422485
5	14.970016394627322	36.28721204898834	32.91696128345701	15.825810272927326
6	35.47141511502643	34.36736261248735	16.23941282516075	13.921809447325472
7	31.202539537691347	29.877295354099857	19.792651182694527	19.127513925514265
8	28.575254456802213	33.04973367586816	18.649774201913228	19.725237665416408
9	26.980356204098232	14.512752242748176	18.587886963508137	39.91900458964545
10	16.798240657656223	26.459036591444246	30.586569073422716	26.156153677476812
11	37.4266173930417	21.206705501764866	21.661631612582344	19.70504549261108
12	24.56941077114262	23.506098036187208	28.214381361420056	23.710109831250115
13	29.534524364513352	19.19992234869686	25.034503818091903	26.231049468697883
14	23.640464547504035	19.27998254262674	24.84544131587785	32.23411159399138
15	25.37245702609679	26.85512930784066	21.83595737113497	25.936456294927584
16	26.031543939896334	25.826078269667313	23.187048861153382	24.955328929282967
17	24.331213981768947	26.005782754892525	24.70923271160334	24.95377055173518
18	25.066129436216222	25.093583735435494	25.511137057883936	24.329149770464344
19	25.698436629876763	24.927662426546544	25.114811986073775	24.259088957502918
20	25.85551169163887	24.33135473376051	24.77593741409458	25.037196160506035
21	26.626542089182127	24.18396138688394	24.489890625691892	24.69960589824204
22	26.029127740644647	24.103609226901927	24.923517717391768	24.943745315061662
23	24.695824184855578	24.2270466198633	25.388593327226083	25.68853586805504
24	24.772020861215953	24.78615553239532	25.23853586061726	25.20328774577147
25	25.0592216642005	24.2138051748062	25.22536469102888	25.50160846996442
26	24.70253614768547	25.070708330263162	25.519826672485816	24.70692884956555
27	25.33560761850106	24.70723892215887	24.882521835085985	25.074631624254085
28	24.951210292936622	24.5226331456847	25.401113563733404	25.12504299764527
29	24.798378383357033	24.825088863713493	25.365922953371438	25.010609799558036
30	24.902766328508285	24.77941533328681	25.493341986560335	24.824476351644567
31	25.338317391503036	24.643846132318668	24.72755535418424	25.290281121994056
32	25.26658332303051	24.63658195207611	24.695883700556635	25.40095102433674
33	24.75452253135643	24.477003159200613	25.180685161817813	25.587789147625145
34	25.417635099733992	24.583693340687578	25.273313332334347	24.725358227244083
35	25.75374610280966	24.381311623962446	25.25021547242573	24.614726800802163
36	24.595208211781554	24.934897839823357	25.09810047997234	25.371793468422748
37	25.673648770243823	24.61542222077372	24.910905203136135	24.80002380584632
38	24.905495284063235	24.49265376610725	25.310614178807068	25.29123677102245
39	25.511770744844032	24.576827783024648	24.700957827667537	25.21044364446378
40	25.674915476153366	24.647699222635673	24.961103443585113	24.716281857625848
41	24.704509940829368	25.11972816154437	25.36221333523212	24.813548562394143
42	25.737828155865355	24.95256388818823	24.935452878475697	24.374155077470718
43	25.362542949848184	24.233226310760816	25.109564402896588	25.294666336494416
44	24.88165014923258	24.80204763043459	24.945133777931293	25.371168442401537
45	25.136317583126093	24.94842037395229	24.883095627776477	25.03216641514514
46	24.94693250012399	24.77958212000765	24.99613861315989	25.27734676670847
47	25.124094707323536	24.75535311196793	25.179428973049873	24.94112320765866
48	25.633049285700622	25.226285140178106	24.35554346339725	24.78512211072402
49	25.077017038691316	25.199309043089862	24.56677030075614	25.15690361746267
50	25.173601885761776	25.25125423421394	24.563294103310216	25.011849776714072
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	110.5
2	78.0
3	250.0
4	422.0
5	437.5
6	453.0
7	377.5
8	302.0
9	427.0
10	552.0
11	960.0
12	1368.0
13	2397.0
14	3426.0
15	4357.0
16	5288.0
17	5446.5
18	5605.0
19	5561.5
20	5518.0
21	6570.5
22	7623.0
23	9048.5
24	10474.0
25	13472.0
26	16470.0
27	20568.5
28	24667.0
29	28592.0
30	32517.0
31	36618.0
32	40719.0
33	46983.0
34	53247.0
35	61943.0
36	70639.0
37	83585.0
38	96531.0
39	107225.0
40	117919.0
41	129014.5
42	140110.0
43	147291.0
44	154472.0
45	167253.0
46	180034.0
47	192639.5
48	205245.0
49	214101.5
50	222958.0
51	218868.0
52	214778.0
53	213511.0
54	212244.0
55	213716.5
56	215189.0
57	207996.5
58	200804.0
59	183856.0
60	166908.0
61	146766.5
62	126625.0
63	108968.0
64	91311.0
65	76860.5
66	62410.0
67	54104.5
68	45799.0
69	40325.0
70	34851.0
71	27361.5
72	19872.0
73	17173.5
74	14475.0
75	10821.5
76	7168.0
77	6223.0
78	5278.0
79	4507.5
80	3737.0
81	2916.0
82	2095.0
83	1721.5
84	1348.0
85	1031.5
86	715.0
87	484.0
88	253.0
89	172.5
90	92.0
91	67.0
92	42.0
93	37.5
94	33.0
95	30.0
96	27.0
97	28.0
98	29.0
99	21.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03312224837364447
2	0.005561703737606611
3	2.83398916565942E-4
4	0.0
5	0.0
6	1.0627459371222824E-4
7	0.0
8	0.0
9	0.0
10	1.0627459371222824E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.084972914148549E-4
17	0.0
18	1.0627459371222824E-4
19	0.0
20	1.41699458282971E-4
21	3.542486457074275E-5
22	0.0
23	7.08497291414855E-5
24	0.0010627459371222823
25	2.479740519951992E-4
26	4.25098374848913E-4
27	3.896735102781702E-4
28	0.0016649686348249092
29	4.959481039903984E-4
30	0.0013107199891174816
31	3.188237811366847E-4
32	8.856216142685687E-4
33	3.5424864570742746E-4
34	6.022226977026267E-4
35	3.188237811366847E-4
36	1.7712432285371373E-4
37	0.0014169945828297098
38	3.542486457074275E-5
39	0.0010627459371222823
40	1.41699458282971E-4
41	8.147718851270832E-4
42	0.004676082123338043
43	0.0037904605090694743
44	0.0035779113216450175
45	0.0019837924159615936
46	0.0019837924159615936
47	0.001133595666263768
48	0.0038967351027817017
49	0.004392683206772101
50	0.0013461448536882244
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2822876.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.77076520315113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7925619524635	38.72068163783122
2	14.808146577336117	15.332581590982281
3	4.480336507649791	6.958513481059303
4	1.9121964330194565	3.959834902246135
5	1.0239595476351908	2.6505584659073156
6	0.6327854382663767	1.9655871809077001
7	0.4181847002323634	1.5154819349095878
8	0.3030998697566839	1.2553369752223174
9	0.22446086443100832	1.045845963877866
>10	1.246634094625348	11.68489927526056
>50	0.08454214809606235	3.02137157209618
>100	0.06250311284674236	6.561632772403502
>500	0.007150846614990365	2.5793663550997983
>1k	0.0034379070263972408	2.748307892196266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3034	0.10747903910763348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0627459371222824E-4
2	0.0	0.0	0.0	0.0	1.0627459371222824E-4
3	0.0	0.0	0.0	0.0	1.0627459371222824E-4
4	0.0	0.0	0.0	0.0	1.0627459371222824E-4
5	0.0	7.08497291414855E-5	0.0	0.0	1.0627459371222824E-4
6	0.0	7.08497291414855E-5	0.0	0.0	1.0627459371222824E-4
7	0.0	7.08497291414855E-5	0.0	3.542486457074275E-5	1.0627459371222824E-4
8	0.0	7.08497291414855E-5	0.0	1.41699458282971E-4	1.7712432285371373E-4
9	0.0	7.08497291414855E-5	0.0	1.41699458282971E-4	2.125491874244565E-4
10	0.0	7.08497291414855E-5	0.0	1.41699458282971E-4	2.125491874244565E-4
11	0.0	7.08497291414855E-5	0.0	1.41699458282971E-4	2.125491874244565E-4
12	0.0	7.08497291414855E-5	0.0	1.41699458282971E-4	3.188237811366847E-4
13	0.0	7.08497291414855E-5	0.0	1.41699458282971E-4	3.896735102781702E-4
14	0.0	7.08497291414855E-5	0.0	1.41699458282971E-4	3.896735102781702E-4
15	0.0	7.08497291414855E-5	0.0	2.479740519951992E-4	5.313729685611412E-4
16	0.0	7.08497291414855E-5	0.0	3.188237811366847E-4	6.730724268441122E-4
17	0.0	7.08497291414855E-5	0.0	3.896735102781702E-4	7.084972914148549E-4
18	0.0	7.08497291414855E-5	0.0	4.959481039903984E-4	8.147718851270831E-4
19	0.0	7.08497291414855E-5	0.0	5.313729685611412E-4	8.147718851270831E-4
20	0.0	1.0627459371222824E-4	0.0	6.376475622733694E-4	8.50196749697826E-4
21	0.0	1.0627459371222824E-4	0.0	8.147718851270831E-4	8.50196749697826E-4
22	0.0	1.0627459371222824E-4	0.0	0.0010273210725515397	8.856216142685687E-4
23	0.0	1.0627459371222824E-4	0.0	0.0014524194474004526	8.856216142685687E-4
24	0.0	1.0627459371222824E-4	0.0	0.0017712432285371374	8.856216142685687E-4
25	0.0	1.0627459371222824E-4	0.0	0.002125491874244565	8.856216142685687E-4
26	0.0	1.0627459371222824E-4	0.0	0.0024443156553812496	9.918962079807968E-4
27	0.0	1.0627459371222824E-4	0.0	0.002798564301088677	9.918962079807968E-4
28	0.0	1.0627459371222824E-4	0.0	0.0035424864570742748	0.0010273210725515397
29	0.0	1.0627459371222824E-4	0.0	0.0051011804981869555	0.0010627459371222825
30	0.0	1.0627459371222824E-4	0.0	0.008360268038695289	0.0010627459371222825
31	0.0	1.0627459371222824E-4	0.0	0.013709422588877442	0.0010981708016930252
32	0.0	1.0627459371222824E-4	0.0	0.01973164956590371	0.0010981708016930252
33	0.0	1.0627459371222824E-4	0.0	0.025505902490934776	0.0010981708016930252
34	0.0	1.0627459371222824E-4	0.0	0.03393702025877155	0.0010981708016930252
35	0.0	1.0627459371222824E-4	0.0	0.04300578558888169	0.0010981708016930252
36	0.0	1.0627459371222824E-4	0.0	0.05458971630351457	0.0010981708016930252
37	0.0	1.0627459371222824E-4	0.0	0.06946815942322652	0.0010981708016930252
38	0.0	1.0627459371222824E-4	0.0	0.09015628033254029	0.0010981708016930252
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3480	0.0	25.4245	1
GGTATCA	1720	0.0	25.20829	1
CGGTACG	90	1.00292455E-5	19.555132	25
TGCGTTA	70	8.13012E-4	18.856064	12
GTATTAG	575	0.0	17.607414	1
TCAACGC	5160	0.0	16.882755	4
CGCAATA	600	0.0	16.500225	36
ATCAACG	5300	0.0	16.436796	3
CAACGCA	5320	0.0	16.416355	5
GTCCTAT	555	0.0	16.259098	1
AACGCAG	5385	0.0	16.259052	6
GGCGTTA	785	0.0	16.25673	42
TATCAAC	5495	0.0	16.054815	2
CGCGAAC	100	4.9487513E-4	15.399119	14
TAGATCG	100	4.9487513E-4	15.399119	5
ACTCGTA	115	8.2277504E-5	15.3034725	11
GCGTTAT	835	0.0	15.283	43
ATACGAA	635	0.0	15.245924	40
CTAGCGG	640	0.0	15.124939	29
CAATACG	670	0.0	15.106022	38
>>END_MODULE