FastQCFastQC Report
Thu 2 Feb 2017
SRR4062053_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062053_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297488
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16920.1304058303429396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTGC1450.014.41578513
GGTATCA3600.013.9815671
AATTGCA1650.013.24425314
GTATCAA11500.013.0479351
GAGTAGG905.423972E-712.6625521
ATTGCAA1750.012.48695715
CGCGTAA550.003064799612.0925810
GAGTCGT550.003067458312.0911796
ATTGATC550.003073670212.08791353
CGAACCT1501.4551915E-1112.03453216
GTTTTCG802.862581E-511.87618215
ATAACCC658.0385327E-411.6894113
TTTATAC1157.0927854E-811.5623523
ACCATAC2150.011.484641
GTATTAG1001.9361269E-611.39629651
AAGGGCG855.326111E-511.17542655
CGTCGTA1451.2132659E-911.1394710
CCGTCGT1451.2132659E-911.139049
TATACTG1451.2169039E-911.136895
GCGTAAC600.00586704311.08529211
Produced by FastQC (version 0.11.5)