FastQCFastQC Report
Thu 2 Feb 2017
SRR4062053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297488
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.028.5790331
GGTATCA5450.026.6557431
ATTATAC1350.024.4436743
TTACACG550.004484604619.9993694
ATATTAT1051.7841958E-618.8667281
GTATTAC1658.185452E-1118.6761531
GTATAGA1658.185452E-1118.6761531
CAACGCA19050.018.4771065
TCAACGC19150.018.3806234
ATCAACG19200.018.3327543
AACGCAG19450.018.2102246
CGAGCCG3450.017.8545115
TATCAAC20200.017.7539672
GTCACGC750.001291212317.59944524
ATACCGC3700.017.24269927
TACCGCA3850.017.14231728
CGCGGAT1206.402144E-616.50138742
ATTACAC1753.6270649E-916.3423423
TATATAG1753.6270649E-916.3423425
ATCACGA2301.8189894E-1216.26035734
Produced by FastQC (version 0.11.5)