##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1297488
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.320806820564044	32.0	32.0	32.0	32.0	32.0
2	31.48295398493088	32.0	32.0	32.0	32.0	32.0
3	31.59049178104152	32.0	32.0	32.0	32.0	32.0
4	31.66662581850468	32.0	32.0	32.0	32.0	32.0
5	31.643549689862258	32.0	32.0	32.0	32.0	32.0
6	35.29235260750003	36.0	36.0	36.0	36.0	36.0
7	35.303824004538	36.0	36.0	36.0	36.0	36.0
8	35.25404242659663	36.0	36.0	36.0	36.0	36.0
9	35.33718076776047	36.0	36.0	36.0	36.0	36.0
10	35.223371622704796	36.0	36.0	36.0	36.0	36.0
11	35.33619887043271	36.0	36.0	36.0	36.0	36.0
12	35.25914228108468	36.0	36.0	36.0	36.0	36.0
13	35.29179845979308	36.0	36.0	36.0	36.0	36.0
14	35.26141436375519	36.0	36.0	36.0	36.0	36.0
15	35.24033054640968	36.0	36.0	36.0	36.0	36.0
16	35.249105194036474	36.0	36.0	36.0	36.0	36.0
17	35.2349671056688	36.0	36.0	36.0	36.0	36.0
18	35.228813676889494	36.0	36.0	36.0	36.0	36.0
19	35.2239226875316	36.0	36.0	36.0	36.0	36.0
20	35.22045213520279	36.0	36.0	36.0	36.0	36.0
21	35.21788717891803	36.0	36.0	36.0	36.0	36.0
22	35.195968671771915	36.0	36.0	36.0	36.0	36.0
23	35.17140274129703	36.0	36.0	36.0	36.0	36.0
24	35.15410855437584	36.0	36.0	36.0	36.0	36.0
25	35.14073887388554	36.0	36.0	36.0	36.0	36.0
26	35.09662979542007	36.0	36.0	36.0	36.0	36.0
27	35.08794686347773	36.0	36.0	36.0	36.0	36.0
28	35.07227350079538	36.0	36.0	36.0	36.0	36.0
29	35.04928215135708	36.0	36.0	36.0	36.0	36.0
30	35.03028313171297	36.0	36.0	36.0	36.0	36.0
31	35.029185626379586	36.0	36.0	36.0	36.0	36.0
32	35.013534614578326	36.0	36.0	36.0	36.0	36.0
33	34.99848322296622	36.0	36.0	36.0	36.0	36.0
34	34.98733090402378	36.0	36.0	36.0	36.0	36.0
35	34.97060165489007	36.0	36.0	36.0	36.0	36.0
36	34.94643804027475	36.0	36.0	36.0	36.0	36.0
37	34.94277866153676	36.0	36.0	36.0	36.0	36.0
38	34.92667909067367	36.0	36.0	36.0	36.0	36.0
39	34.915995369514015	36.0	36.0	36.0	36.0	36.0
40	34.89837902161715	36.0	36.0	36.0	36.0	36.0
41	34.87725435610965	36.0	36.0	36.0	32.0	36.0
42	34.84642940821033	36.0	36.0	36.0	32.0	36.0
43	34.84924022418704	36.0	36.0	36.0	32.0	36.0
44	34.8141138877585	36.0	36.0	36.0	32.0	36.0
45	34.80047368453504	36.0	36.0	36.0	32.0	36.0
46	34.78043496356036	36.0	36.0	36.0	32.0	36.0
47	34.760175045934915	36.0	36.0	36.0	32.0	36.0
48	34.73246688986719	36.0	36.0	36.0	32.0	36.0
49	34.728012898770544	36.0	36.0	36.0	32.0	36.0
50	34.31789966458264	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	14.0
21	23.0
22	75.0
23	208.0
24	612.0
25	1403.0
26	2797.0
27	5536.0
28	9519.0
29	14903.0
30	22503.0
31	33028.0
32	50618.0
33	86633.0
34	201111.0
35	868502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.010784281223955	17.49207639045759	11.863557409644638	26.633581918673816
2	16.463006696345992	19.531394517135492	36.525915968873704	27.479682817644814
3	18.771084058775244	22.782228696285507	28.08063291675819	30.366054328181058
4	12.9579718332438	15.61896188555261	34.475875288153176	36.94719099305041
5	15.020177450581432	36.172357663423476	32.94411971440198	15.86334517159311
6	35.82282002924123	34.35528635057261	15.985797116416938	13.836096503769221
7	31.241445007583884	29.82239527456131	19.802726499204617	19.133433218650193
8	28.6331742567176	33.031288150641856	18.462136065998298	19.87340152664225
9	27.022677666383043	14.39689615626503	18.182056404375224	40.39836977297671
10	16.364402880337146	27.00951917051963	30.826436025948624	25.799641923194606
11	37.68512695300458	21.18293194233781	21.369754479424856	19.762186625232758
12	24.684621360659982	23.291467820896994	28.278951327488194	23.744959490954827
13	29.82470743467377	19.358868829615382	24.512982008311447	26.303441727399406
14	23.580256618943682	19.08996460853588	24.790595365814557	32.53918340670588
15	25.31954052754245	26.952542142971648	21.590719914172617	26.137197415313285
16	26.240652093322225	25.697677734505962	22.965772961957885	25.09589721021393
17	24.592520316180188	25.85233928945778	24.615564845301073	24.939575549060955
18	25.129731550296842	25.103218760573327	25.328731617349536	24.438318071780294
19	25.868832698260025	24.818880791190363	24.74281072349031	24.569475787059304
20	25.891648760215922	24.494251952239875	24.424964007263288	25.18913528028091
21	26.550863322715372	24.185290488459614	24.3715736650926	24.892272523732416
22	25.99183961624308	24.201611113166365	24.69502608116607	25.11152318942449
23	24.959768414043136	24.290937565511204	25.045472482211785	25.70382153823388
24	25.1569588292328	24.699531551306617	24.819919320155932	25.323590299304648
25	25.205281598414164	24.339607517923124	24.874025615690652	25.581085267972064
26	25.13740085596629	25.13778621806408	24.944180300135415	24.780632625834212
27	25.49728975254396	24.831076784821075	24.441322159904985	25.23031130272998
28	25.17220038058654	24.63839268854564	24.908687747163523	25.2807191837043
29	25.03317975333686	24.92003717982116	24.927744439597436	25.119038627244546
30	25.228366459186912	24.659261520058944	24.985510329285475	25.126861691468665
31	25.387481290684573	24.690361793073045	24.494675070636816	25.427481845605566
32	25.374476772998673	24.66779757945613	24.241508712678527	25.71621693486667
33	24.977880266731614	24.548587882393925	24.879381942282137	25.59414990859232
34	25.662380662808417	24.60533079918827	24.661131316961583	25.07115722104173
35	25.787370017664955	24.41047442588887	24.969633536906812	24.832522019539354
36	25.03325662088069	24.879728952759415	24.612057471140343	25.474956955219557
37	25.68142388207176	24.685637476714245	24.494187520761606	25.13875112045239
38	25.20108085778057	24.565622186871863	24.733870371055456	25.499426584292106
39	25.616907865175463	24.700746601679104	24.300431837432438	25.381913695712992
40	25.808293885251942	24.5897065556006	24.817377605615786	24.784621953531676
41	25.069962920230328	24.90109620778236	24.967995480445452	25.060945391541868
42	25.91311988407763	24.72306577669529	24.47711612276672	24.88669821646036
43	25.422605339178745	24.34855729951874	24.676051387822675	25.552785973479843
44	25.11152697149164	24.75313000021581	24.68676875456667	25.448574273725878
45	25.321765027326613	24.90217372035827	24.62840657811882	25.14765467419629
46	25.308411704769497	24.792478191905072	24.577056296408195	25.32205380691724
47	25.407637597920885	24.738492272689435	24.76724039596879	25.08662973342089
48	25.693402865022097	25.160739362530272	24.149133448739903	24.996724323707724
49	25.235792159144776	24.972734664935047	24.330698647716456	25.46077452820372
50	25.230949463186047	25.12489691476489	24.568352254772748	25.075801367276313
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	21.5
2	36.0
3	96.0
4	156.0
5	169.5
6	183.0
7	153.5
8	124.0
9	170.5
10	217.0
11	374.5
12	532.0
13	912.5
14	1293.0
15	1686.0
16	2079.0
17	2087.5
18	2096.0
19	2162.5
20	2229.0
21	2755.5
22	3282.0
23	4154.5
24	5027.0
25	6383.5
26	7740.0
27	9281.0
28	10822.0
29	12742.5
30	14663.0
31	16509.0
32	18355.0
33	21194.0
34	24033.0
35	28175.0
36	32317.0
37	36690.5
38	41064.0
39	45424.5
40	49785.0
41	55100.0
42	60415.0
43	63279.0
44	66143.0
45	73968.5
46	81794.0
47	88053.0
48	94312.0
49	98453.0
50	102594.0
51	102239.0
52	101884.0
53	102184.0
54	102484.0
55	101325.0
56	100166.0
57	96902.5
58	93639.0
59	85926.0
60	78213.0
61	69784.5
62	61356.0
63	53247.0
64	45138.0
65	38525.0
66	31912.0
67	26995.5
68	22079.0
69	18985.5
70	15892.0
71	12444.5
72	8997.0
73	7644.0
74	6291.0
75	4619.5
76	2948.0
77	2591.5
78	2235.0
79	1808.0
80	1381.0
81	1077.0
82	773.0
83	606.5
84	440.0
85	331.5
86	223.0
87	147.5
88	72.0
89	49.5
90	27.0
91	19.0
92	11.0
93	9.5
94	8.0
95	8.5
96	9.0
97	8.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03198488155574464
2	0.004932608239922065
3	2.3121601124634678E-4
4	7.707200374878226E-5
5	0.0
6	3.853600187439113E-4
7	0.0
8	0.0
9	0.0
10	7.707200374878226E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.853600187439113E-4
17	0.0
18	7.707200374878226E-5
19	0.0
20	3.0828801499512904E-4
21	7.707200374878226E-5
22	0.0
23	0.0
24	0.0010790080524829518
25	1.5414400749756452E-4
26	5.395040262414759E-4
27	3.853600187439113E-4
28	0.0013102240637292983
29	7.707200374878227E-4
30	9.248640449853871E-4
31	4.6243202249269356E-4
32	8.477920412366049E-4
33	3.0828801499512904E-4
34	6.936480337390404E-4
35	3.0828801499512904E-4
36	1.5414400749756452E-4
37	0.0016185120787244277
38	0.0
39	6.936480337390404E-4
40	1.5414400749756452E-4
41	0.001156080056231734
42	0.005240896254917193
43	0.004624320224926936
44	0.0033911681649464198
45	0.002697520131207379
46	0.0026204481274585967
47	9.248640449853871E-4
48	0.003545312172443984
49	0.00408481619868546
50	0.0013872960674780807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1297488.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.543385691895445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24427199206309	41.2377823466915
2	14.947107797831183	16.604259467865507
3	4.909773565626379	8.18116340446376
4	2.0906807358972266	4.644939458902217
5	1.0609610112668482	2.9464683326428984
6	0.63648452634604	2.1211503320256893
7	0.4024942212188235	1.564912423754134
8	0.2813271533170496	1.2500690065833509
9	0.21676641554252504	1.0835946561175334
>10	1.0829043975741544	10.813925634955767
>50	0.07429714367197936	2.8586263170422073
>100	0.04888092395330937	5.155642938326652
>500	0.0040501156911900895	1.5374656806287978
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3121601124634678E-4
2	0.0	0.0	0.0	0.0	2.3121601124634678E-4
3	0.0	0.0	0.0	0.0	2.3121601124634678E-4
4	0.0	0.0	0.0	0.0	3.0828801499512904E-4
5	0.0	0.0	0.0	0.0	3.853600187439113E-4
6	0.0	0.0	0.0	0.0	4.6243202249269356E-4
7	0.0	0.0	0.0	0.0	4.6243202249269356E-4
8	0.0	0.0	0.0	0.0	4.6243202249269356E-4
9	0.0	0.0	0.0	0.0	4.6243202249269356E-4
10	0.0	0.0	0.0	0.0	5.395040262414758E-4
11	0.0	0.0	0.0	0.0	5.395040262414758E-4
12	0.0	0.0	0.0	0.0	0.001156080056231734
13	0.0	0.0	0.0	0.0	0.0013872960674780807
14	0.0	0.0	0.0	0.0	0.0013872960674780807
15	0.0	1.5414400749756452E-4	0.0	0.0	0.001772656086221992
16	0.0	1.5414400749756452E-4	0.0	0.0	0.001772656086221992
17	0.0	1.5414400749756452E-4	0.0	1.5414400749756452E-4	0.0019268000937195566
18	0.0	1.5414400749756452E-4	0.0	1.5414400749756452E-4	0.002003872097468339
19	0.0	2.3121601124634678E-4	0.0	1.5414400749756452E-4	0.002003872097468339
20	0.0	2.3121601124634678E-4	0.0	1.5414400749756452E-4	0.002158016104965903
21	0.0	2.3121601124634678E-4	0.0	1.5414400749756452E-4	0.002312160112463468
22	0.0	2.3121601124634678E-4	0.0	1.5414400749756452E-4	0.002312160112463468
23	0.0	2.3121601124634678E-4	0.0	3.0828801499512904E-4	0.002312160112463468
24	0.0	2.3121601124634678E-4	0.0	5.395040262414758E-4	0.002312160112463468
25	0.0	2.3121601124634678E-4	0.0	7.707200374878227E-4	0.002312160112463468
26	0.0	2.3121601124634678E-4	0.0	9.248640449853871E-4	0.0024663041199610323
27	0.0	2.3121601124634678E-4	0.0	0.0010790080524829516	0.0026975201312073793
28	0.0	2.3121601124634678E-4	0.0	0.0013102240637292986	0.0026975201312073793
29	0.0	2.3121601124634678E-4	0.0	0.001772656086221992	0.0026975201312073793
30	0.0	2.3121601124634678E-4	0.0	0.0026975201312073793	0.0026975201312073793
31	0.0	2.3121601124634678E-4	0.0	0.004701392228675718	0.0028516641387049436
32	0.0	2.3121601124634678E-4	0.0	0.006705264326144057	0.002928736142453726
33	0.0	2.3121601124634678E-4	0.0	0.008709136423612396	0.002928736142453726
34	0.0	2.3121601124634678E-4	0.0	0.010713008521080734	0.003237024157448855
35	0.0	2.3121601124634678E-4	0.0	0.013487600656036896	0.003237024157448855
36	0.0	2.3121601124634678E-4	0.0	0.018574352903456524	0.003237024157448855
37	0.0	2.3121601124634678E-4	0.0	0.02512547322210302	0.003314096161197637
38	0.0	2.3121601124634678E-4	0.0	0.03437411367195689	0.003468240168695202
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1240	0.0	28.579033	1
GGTATCA	545	0.0	26.655743	1
ATTATAC	135	0.0	24.443674	3
TTACACG	55	0.0044846046	19.999369	4
ATATTAT	105	1.7841958E-6	18.866728	1
GTATTAC	165	8.185452E-11	18.676153	1
GTATAGA	165	8.185452E-11	18.676153	1
CAACGCA	1905	0.0	18.477106	5
TCAACGC	1915	0.0	18.380623	4
ATCAACG	1920	0.0	18.332754	3
AACGCAG	1945	0.0	18.210224	6
CGAGCCG	345	0.0	17.85451	15
TATCAAC	2020	0.0	17.753967	2
GTCACGC	75	0.0012912123	17.599445	24
ATACCGC	370	0.0	17.242699	27
TACCGCA	385	0.0	17.142317	28
CGCGGAT	120	6.402144E-6	16.501387	42
ATTACAC	175	3.6270649E-9	16.342342	3
TATATAG	175	3.6270649E-9	16.342342	5
ATCACGA	230	1.8189894E-12	16.260357	34
>>END_MODULE