FastQCFastQC Report
Thu 2 Feb 2017
SRR4062052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029760
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16960.1646985705407085No Hit
TATCAACGCAGAGTACTTTTTTTTT12200.11847420758234928No Hit
GCGCAAGACGGACCAGAGCGAAAGC10550.10245105655686762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG851.022272E-915.6430161
GGTATCA4550.014.4028711
CGCAAGA1600.014.2477042
GCACCGT551.9569698E-413.8199846
GCGTAAC707.2506937E-613.57254111
ACGACTG500.001497472613.3017355
GTCCTAG1001.0091753E-813.2965631
TCGGCGT655.4497763E-513.15364513
CGCGTAA751.4761885E-512.66770410
GCGTTAT1051.9976142E-812.6633931
AAGACGG2050.012.5138285
CAAGACG2050.012.5120044
GTATCAA12300.012.2000991
CGGCGTC1052.7184797E-711.76115314
TCGCGTA907.438739E-611.6131919
GCGCAAG2050.011.5823721
ACGGACC2250.011.4020438
GCGGTCG1001.9231502E-611.4020429
CGTCGTA1251.8260835E-811.40093410
GTAGGAC752.0737469E-411.3992713
Produced by FastQC (version 0.11.5)