##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062052_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1029760
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2021500155376	32.0	32.0	32.0	32.0	32.0
2	30.657990211311372	32.0	32.0	32.0	32.0	32.0
3	30.696595323182102	32.0	32.0	32.0	32.0	32.0
4	30.703390110316967	32.0	32.0	32.0	32.0	32.0
5	30.575917689558732	32.0	32.0	32.0	32.0	32.0
6	34.24048030609074	36.0	36.0	36.0	32.0	36.0
7	34.18580251709136	36.0	36.0	36.0	32.0	36.0
8	34.10866027035426	36.0	36.0	36.0	32.0	36.0
9	34.29535425730267	36.0	36.0	36.0	32.0	36.0
10	33.914945229956494	36.0	36.0	36.0	32.0	36.0
11	34.2691937927284	36.0	36.0	36.0	32.0	36.0
12	34.05952552050963	36.0	36.0	36.0	32.0	36.0
13	34.16179983685519	36.0	36.0	36.0	32.0	36.0
14	34.05327454940957	36.0	36.0	36.0	32.0	36.0
15	33.991140654133005	36.0	36.0	36.0	32.0	36.0
16	33.99855208980733	36.0	36.0	36.0	32.0	36.0
17	33.91655142945929	36.0	36.0	36.0	32.0	36.0
18	33.92640323958981	36.0	36.0	36.0	32.0	36.0
19	33.93235802517091	36.0	36.0	36.0	32.0	36.0
20	33.927197599440646	36.0	36.0	36.0	32.0	36.0
21	33.9153171612803	36.0	36.0	36.0	32.0	36.0
22	33.87625951678061	36.0	36.0	36.0	32.0	36.0
23	33.83674739745183	36.0	36.0	36.0	32.0	36.0
24	33.80414562616532	36.0	36.0	36.0	32.0	36.0
25	33.431898694841514	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	18.0
5	81.0
6	223.0
7	53.0
8	171.0
9	131.0
10	83.0
11	32.0
12	46.0
13	49.0
14	148.0
15	194.0
16	332.0
17	484.0
18	653.0
19	965.0
20	1547.0
21	2249.0
22	3370.0
23	5036.0
24	7194.0
25	9886.0
26	13828.0
27	17715.0
28	23046.0
29	29897.0
30	39520.0
31	52228.0
32	73053.0
33	102396.0
34	215163.0
35	429968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65097480160195	17.149535614538703	11.551175627435061	25.64831395642429
2	17.23228444713763	18.64368264807591	37.47158315683573	26.652449747950733
3	20.055081116460823	21.75053459369908	27.44704074953824	30.74734354030186
4	13.388244823276102	14.956436949391886	33.74900573686189	37.90631249047013
5	15.565662904582696	35.66107826546492	32.703221507022576	16.0700373229298
6	36.09232975802016	33.56505819682167	15.900491111599136	14.442120933559032
7	32.040643324882176	29.32146412406091	18.97417601219455	19.66371653886236
8	28.7101424900815	33.11530934745434	18.028198589778864	20.146349572685303
9	26.655369011367657	15.139052552570071	18.47477764425414	39.73080079180813
10	17.56224024022856	26.298239711227843	29.503458925622887	26.636061122920708
11	37.37419807196906	21.399918117519835	21.056634085478695	20.169249725032408
12	24.90200444784575	23.365679239723715	27.646560601655253	24.08575571077528
13	29.879902802073477	19.184435885443357	24.53061355461841	26.405047757864757
14	23.67419878292202	19.28744838502366	24.42050734592802	32.6178454861263
15	25.53339213182791	26.61695049743721	21.432829202690467	26.41682816804441
16	26.244105436124805	25.685519548075337	22.73459148849284	25.335783527307022
17	24.490595611285265	26.020558973840398	24.27716888228568	25.211676532588655
18	25.091169999601387	24.91305916060406	25.2736558319147	24.72211500787985
19	25.992069856804438	24.739752686071117	24.58315228538406	24.68502517174038
20	26.073927044988633	24.304437041868404	24.473765298594145	25.14787061454882
21	26.685309981560806	23.985431386671877	24.32835951465172	25.000899117115594
22	26.311665688077674	23.893935210928053	24.624474575480317	25.169924525513952
23	24.991009659027956	24.135226391364608	25.04271626872664	25.831047680880804
24	24.97385272767567	24.56453613378072	24.916989700501947	25.54462143804166
25	25.215416900348846	24.327505615662233	24.796198359478197	25.66087912451073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	90.0
1	90.0
2	189.0
3	288.0
4	288.0
5	288.0
6	622.5
7	957.0
8	957.0
9	957.0
10	1181.5
11	1406.0
12	1406.0
13	1406.0
14	1917.5
15	2429.0
16	2429.0
17	2429.0
18	3996.5
19	5564.0
20	5564.0
21	5564.0
22	8586.0
23	11608.0
24	11608.0
25	11608.0
26	17105.5
27	22603.0
28	22603.0
29	22603.0
30	28897.5
31	35192.0
32	35192.0
33	35192.0
34	44689.5
35	54187.0
36	54187.0
37	54187.0
38	65458.0
39	76729.0
40	76729.0
41	76729.0
42	89871.0
43	103013.0
44	103013.0
45	103013.0
46	116622.0
47	130231.0
48	130231.0
49	130231.0
50	137127.5
51	144024.0
52	144024.0
53	144024.0
54	138377.0
55	132730.0
56	132730.0
57	132730.0
58	123234.5
59	113739.0
60	113739.0
61	113739.0
62	99679.0
63	85619.0
64	85619.0
65	85619.0
66	70211.5
67	54804.0
68	54804.0
69	54804.0
70	41465.5
71	28127.0
72	28127.0
73	28127.0
74	21119.5
75	14112.0
76	14112.0
77	14112.0
78	10696.5
79	7281.0
80	7281.0
81	7281.0
82	5053.0
83	2825.0
84	2825.0
85	2825.0
86	2043.5
87	1262.0
88	1262.0
89	1262.0
90	841.5
91	421.0
92	421.0
93	421.0
94	273.0
95	125.0
96	125.0
97	125.0
98	259.5
99	394.0
100	394.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011653200745804849
2	9.71100062150404E-5
3	5.826600372902424E-4
4	0.010002330640149161
5	0.035542262274704785
6	0.06467526413921691
7	0.1072094468614046
8	0.10944297700435053
9	0.11925108763206961
10	0.1365366687383468
11	0.14148927905531386
12	0.13566267868241144
13	0.13041873834679923
14	0.11954241765071473
15	0.13488579863269112
16	0.12332970789310131
17	0.12663144810441268
18	0.11526957737725296
19	0.09973197638284649
20	0.09604179614667495
21	0.09448803604723431
22	0.10371348663766314
23	0.08526258545680547
24	0.09439092604101927
25	0.09138051584835302
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1029760.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.915772743318385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5989919385932	46.307203469080704
2	12.705191631218668	14.97072365610379
3	3.8360319634797664	6.780083621894378
4	1.6245234507890443	3.8284021777151476
5	0.8655793129949076	2.5498137047862817
6	0.5117471155645605	1.80899860575902
7	0.35361983237487116	1.4583649977209774
8	0.2527144569936632	1.1911094013751813
9	0.18136535945876323	0.9616752271244469
>10	0.9522591030152837	10.018143690486562
>50	0.0661948249615382	2.7330946663976188
>100	0.04764514771565571	5.563223178311858
>500	0.003474124785650335	1.3439727673646287
>1k	6.617380544095877E-4	0.4851908358794055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1696	0.1646985705407085	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1220	0.11847420758234928	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1055	0.10245105655686762	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.884400248601616E-4
2	0.0	0.0	0.0	0.0	3.884400248601616E-4
3	0.0	0.0	0.0	0.0	3.884400248601616E-4
4	0.0	0.0	0.0	9.71100062150404E-5	3.884400248601616E-4
5	0.0	0.0	0.0	9.71100062150404E-5	3.884400248601616E-4
6	0.0	0.0	0.0	9.71100062150404E-5	3.884400248601616E-4
7	0.0	0.0	0.0	9.71100062150404E-5	3.884400248601616E-4
8	0.0	0.0	0.0	9.71100062150404E-5	3.884400248601616E-4
9	0.0	0.0	0.0	9.71100062150404E-5	4.85550031075202E-4
10	0.0	0.0	0.0	9.71100062150404E-5	5.826600372902424E-4
11	0.0	0.0	0.0	9.71100062150404E-5	5.826600372902424E-4
12	0.0	0.0	0.0	9.71100062150404E-5	0.0011653200745804849
13	0.0	0.0	0.0	9.71100062150404E-5	0.0018450901180857675
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	85	1.022272E-9	15.643016	1
GGTATCA	455	0.0	14.402871	1
CGCAAGA	160	0.0	14.247704	2
GCACCGT	55	1.9569698E-4	13.819984	6
GCGTAAC	70	7.2506937E-6	13.572541	11
ACGACTG	50	0.0014974726	13.301735	5
GTCCTAG	100	1.0091753E-8	13.296563	1
TCGGCGT	65	5.4497763E-5	13.153645	13
CGCGTAA	75	1.4761885E-5	12.667704	10
GCGTTAT	105	1.9976142E-8	12.663393	1
AAGACGG	205	0.0	12.513828	5
CAAGACG	205	0.0	12.512004	4
GTATCAA	1230	0.0	12.200099	1
CGGCGTC	105	2.7184797E-7	11.761153	14
TCGCGTA	90	7.438739E-6	11.613191	9
GCGCAAG	205	0.0	11.582372	1
ACGGACC	225	0.0	11.402043	8
GCGGTCG	100	1.9231502E-6	11.402042	9
CGTCGTA	125	1.8260835E-8	11.400934	10
GTAGGAC	75	2.0737469E-4	11.399271	3
>>END_MODULE